LOCUS       sequence-426207-        5747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human SEC31A fragment M1 to T338. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_SEC31A:1-338
FEATURES             Location/Qualifiers
     source          1..5747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  18..242
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(309..842)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1012..1017,1018..1034,1035..1040))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1392..1483
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1408..1425
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1542..1559
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1572..1592
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2744..2878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2781..2800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2835..2854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2907..3072
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3256..3710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3343..3362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3552..3573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3736..3840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3841..3909,3910..4701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4059..4078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4872..5460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5361..5380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5614..5631
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgccctgcgt aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta
       61 aactatgaca ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt
      121 atgctgtgaa aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga
      181 agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta aagactatat
      241 tcgcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga
      301 acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg
      361 cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc
      421 acataagcac caagcgcgtt ggcctcatgc ttgagcagat tgatgagcgc ggtggcaatg
      481 ccctgcctcc ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc
      541 tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag
      601 gcagcaagcg cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc
      661 tgatgttggg agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc
      721 cgcatggatt tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag
      781 ccacctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
      841 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
      901 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
      961 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     1021 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     1081 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     1141 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     1201 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     1261 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     1321 catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctacg tatactccgg
     1381 aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt
     1441 ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata
     1501 ccgtcccacc atcgggcgcg gatctcggtc cgaaaaccat gcatcatcat catcatcaca
     1561 gcagcggcag agaaaacttg tatttccagg gcatgaagtt aaaggaagta gatcgtacag
     1621 ccatgcaggc atggagccct gcccagaatc accccattta cctagcaaca ggaacatctg
     1681 ctcagcaatt ggatgcaaca tttagtacga atgcttccct tgagatattt gaattagacc
     1741 tctctgatcc atccttggat atgaaatctt gtgccacatt ctcctcttct cacaggtacc
     1801 acaagttgat ttgggggcct tataaaatgg attccaaagg agatgtctct ggagttctga
     1861 ttgcaggtgg tgaaaatgga aatattattc tctatgatcc ttctaaaatt atagctggag
     1921 acaaggaagt tgtgattgcc cagaatgaca agcatactgg cccagtgaga gccttggatg
     1981 tgaacatttt ccagactaat ctggtagctt ctggtgctaa tgaatctgaa atctacatat
     2041 gggatctaaa taattttgca accccaatga caccaggagc caaaacacag ccgccagaag
     2101 atatcagctg cattgcatgg aacagacaag ttcagcatat tttagcatca gccagtccca
     2161 gtggccgggc cactgtatgg gatcttagga aaaatgagcc aatcatcaaa gtcagtgacc
     2221 atagtaacag aatgcattgt tctgggttgg catggcatcc tgatgttgct actcagatgg
     2281 tccttgcctc cgaggatgac cggttaccag tgatccagat gtgggatctt cgatttgctt
     2341 cctctccact tcgtgtcctg gaaaaccatg ccagggggat tttggcaatt gcttggagca
     2401 tggcagatcc tgaattgtta ctgagctgtg gaaaagatgc taagattctc tgctccaatc
     2461 caaacacagg agaggtgtta tatgaacttc ccaccaacac acagtggtgc ttcgatattc
     2521 agtggtgtcc ccgaaatcct gctgtcttat cagctgcttc gtttgatggg cgtatcagtg
     2581 tttattctat catgggagga agcacatgat gacgaagctt gtcgagaagt actagaggat
     2641 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2701 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2761 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2821 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     2881 atcactgata tcgcctagga gatccgaacc agataagtga aatctagttc caaactattt
     2941 tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc
     3001 actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt
     3061 tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa
     3121 ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt
     3181 ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc
     3241 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3301 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3541 cgttcttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3601 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3661 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     3721 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3781 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3841 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3901 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3961 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4021 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4081 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4141 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4201 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4261 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4321 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4381 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4441 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4501 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4561 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4621 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4681 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4741 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4801 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4861 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4921 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4981 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5041 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5101 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5161 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5221 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5281 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5341 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5401 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5461 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5521 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5581 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5641 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac
     5701 cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatgga
//
