LOCUS       sequence-426210-        5789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human STXBP5 fragment G31 to G382. N-terminal 
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_STXBP5:31-382
FEATURES             Location/Qualifiers
     source          1..5789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..485
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(118..137)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     327..348
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        511..615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(616..684,685..1476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(834..853)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1647..2235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2136..2155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2389..2406
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2540..2764
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2831..3364)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3534..3539,3540..3556,3557..3562))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3914..4005
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3930..3947
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4064..4081
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4094..4114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5308..5442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5345..5364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5399..5418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5471..5636
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 cgcttatttg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
       61 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      121 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      181 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      241 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      301 tgacgttgga gtccacgttc ttaatagtgg actcttgttc caaactggaa caacactcaa
      361 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
      421 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
      481 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      541 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      601 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      661 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      721 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      781 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      841 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      901 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      961 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1021 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1081 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1141 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1201 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1261 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1321 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1381 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1441 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2161 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2221 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2281 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2341 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2401 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2461 tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg
     2521 gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac aaaatagatc
     2581 taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa atcagtccag
     2641 ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc ttcattttct
     2701 gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg taaagactat
     2761 attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt
     2821 gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta
     2881 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
     2941 tcacataagc accaagcgcg ttggcctcat gcttgagcag attgatgagc gcggtggcaa
     3001 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
     3061 gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
     3121 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
     3181 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
     3241 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
     3301 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     3361 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     3421 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     3481 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     3541 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     3601 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     3661 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     3721 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     3781 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     3841 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc
     3901 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     3961 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     4021 taccgtccca ccatcgggcg cggatctcgg tccgaaaacc atgcatcatc atcatcatca
     4081 cagcagcggc agagaaaact tgtatttcca gggcgggaac cgggagccgg agatccagga
     4141 aacgctccag tccgagcact ttcagctctg caagactgtt cgccatggat ttccctatca
     4201 accctcagcc ctggcctttg atcctgtaca gaagatcctg gcagtgggaa ctcagactgg
     4261 tgctttaagg ctctttggtc gtccaggagt agaatgttat tgccagcatg acagtggagc
     4321 tgcagtaatc cagctccagt tcctgattaa tgagggagcg cttgtgagtg ccttggctga
     4381 tgacacctta cacttatgga atttacgtca gaagaggcct gccatactac attcgcttaa
     4441 attttgcaga gaaagggtta cattttgcca tctgcctttc cagagtaagt ggctctatgt
     4501 gggcactgaa cgaggtaata tacatattgt caatgtggag tccttcacac tctcaggcta
     4561 cgtcattatg tggaataaag ccattgaact gtcatctaaa tctcacccag gacctgtggt
     4621 ccatataagt gataatccaa tggacgaggg aaagcttttg attggctttg aatctggaac
     4681 agtagtttta tgggacctca aatcaaagaa agccgactac agatacacat atgatgaggc
     4741 tatccactct gttgcttggc atcatgaagg aaaacaattt atttgcagtc attcagatgg
     4801 caccttgact atatggaatg taaggtcccc tgctaaacca gtacagacaa tcactccaca
     4861 tggaaaacag ttaaaggatg ggaagaagcc agaaccatgc aaacctatcc tcaaggtgga
     4921 attcaaaacg actagatctg gggagccttt tattatttta tcaggaggtt tgtcatatga
     4981 tactgtagga agaagacctt gcttaacagt gatgcatggg aaaagcactg ctgtgctaga
     5041 aatggactat tcaattgttg attttctaac gctgtgtgaa acaccatacc caaatgattt
     5101 tcaagaacca tatgctgtgg ttgttcttct agaaaaggat ttagtactta tagaccttgc
     5161 acaaaatgga tgatgacgaa gcttgtcgag aagtactaga ggatcataat cagccatacc
     5221 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5281 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5401 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gatatcgcct
     5461 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     5521 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     5581 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
     5641 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
     5701 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt
     5761 attaatgttt gtaattgact gaatatcaa
//
