LOCUS       sequence-426211-        5756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full length human THOC6. N-terminal 6X His tag 
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_THOC6:1-341
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(416..436)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(449..466)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(525..616)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(583..600)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(968..973,974..990,991..996)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1166..1699
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  1766..1990
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2124..2141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2295..2883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2375..2394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3054..3845,3846..3914))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3677..3696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3915..4019)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4045..4499)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4182..4203)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4393..4412
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  4683..4848
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    4877..5011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4901..4920)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4955..4974
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tctccttggg gaccagcagc aaagcgttga tctcaggcac ttccaggctg gtcctgtatg
       61 gaggctgacg acgccacagc tccttacagc ccttcttgag catctccgcc caaagccagg
      121 ccttcacctc cccatcccca gcactaagca gatgtcgatc ggtggaaacc atgctataga
      181 cgggcccatc atgggcttgg aaagtcacca ccggcttctt actttcctct ttggcttctg
      241 agctcaaagc agaggacaag ctgaagatgg caatctgccc gtaattgttg ccagccgcca
      301 gaaacttccc acatggtgag acgctctggg agaagatggt catatggagc cgctgcaagg
      361 cctggaacac ctctgtctga cccagaggca ccgcgagcgg cacagctcgc tccatgccct
      421 ggaaatacaa gttttctctg ccgctgctgt gatgatgatg atgatgcatg gttttcggac
      481 cgagatccgc gcccgatggt gggacggtat gaataatccg gaatatttat aggttttttt
      541 attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg gttatcattt
      601 taattatctc catgatctat taatattccg gagtatacgt agccaaccac tagaactata
      661 gctagagtcc tgggcgaaca aacgatgctc gccttccaga aaaccgagga tgcgaaccac
      721 ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg
      781 aagccagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg ccgccaatgc
      841 ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc
      901 gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
      961 aacccagttg acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc
     1021 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     1081 catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc
     1141 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     1201 cagtcgccct aaaacaaagt taggtggctc aagtatgggc atcattcgca catgtaggct
     1261 cggccctgac caagtcaaat ccatgcgggc tgctcttgat cttttcggtc gtgagttcgg
     1321 agacgtagcc acctactccc aacatcagcc ggactccgat tacctcggga acttgctccg
     1381 tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa gcggttgttg gcgctctcgc
     1441 ggcttacgtt ctgcccaagt ttgagcagcc gcgtagtgag atctatatct atgatctcgc
     1501 agtctccggc gagcaccgga ggcagggcat tgccaccgcg ctcatcaatc tgctcaagca
     1561 tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa gcagattacg gtgacgatcc
     1621 cgcagtggct ctctatacaa agttgggcat acgggaagaa gtgatgcact ttgatatcga
     1681 cccaagtacc gccacctaac aattcgttca agccgagatc ggcttcccgg ccgcggagtt
     1741 gttcggtaaa ttgtcacaac gccgcgaata tagtctttac catgcccttg gccacgcccc
     1801 tctttaatac gacgggcaat ttgcacttca gaaaatgaag agtttgcttt agccataaca
     1861 aaagtccagt atgctttttc acagcataac tggactgatt tcagtttaca actattctgt
     1921 ctagtttaag actttattgt catagtttag atctattttg ttcagtttaa gactttattg
     1981 tccgcccaca cccgcttacg cagggcatcc atttattact caaccgtaac cgattttgcc
     2041 aggttacgcg gctggtctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2101 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2161 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2221 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2281 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2341 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2401 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2461 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2521 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2581 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2641 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2701 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2761 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2821 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2881 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2941 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3001 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3061 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3121 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3181 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3241 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3301 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3361 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3421 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3481 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3541 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3601 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3661 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3721 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3781 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3841 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3901 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3961 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4021 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     4081 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4141 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4201 cactattaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4261 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4321 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4381 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4441 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4501 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa
     4561 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg
     4621 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc
     4681 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt
     4741 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
     4801 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag
     4861 gcgatatcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4921 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4981 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     5041 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     5101 tatggctgat tatgatcctc tagtacttct cgacaagctt cgtcatcaga aggacaggga
     5161 gaaggctcgg taacccaggt tggtgaagac atccacccgg cagctgttgc ctgcagctgt
     5221 caggaccttg cactcaggcg cggcaggctg ctggttgagg ctgaggctga gcagccctgg
     5281 ggaggagcca ggcacctggg ccttcagctc cccgctcagc tgccactggt tgacgcagcg
     5341 gccctggcca gctgacagaa tcaggtcctg gtagaaggtg acgtgcttct gtggcgcccg
     5401 gatggggaag atggtggtgg gtgtggagga tcggaggtgc cagagggtga gggctgggcc
     5461 ccctccacag accatccagt cggaatcagt tgccaaacat ccaatccagc gcccattgtg
     5521 gggcctcgag cactcctcgt gcttatagac ctcgatcgtc tggacctcct tggctgtgcg
     5581 caggtcccaa agtcgaacag ctccatcctc gccacctgac agcacctctg ggctcctttc
     5641 ccgcagtgcc aggcagtgga tgtagtctgt gtggccccgg aggaccctcg tgaaagtccc
     5701 agtttcaagg tccatagtgt gcaactgaca gtctccccca gccaggatga gggaat
//
