LOCUS       sequence-426218-        5636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human TLE3 fragment G472 to Y772. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_TLE3:472-772
FEATURES             Location/Qualifiers
     source          1..5636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  73..297
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(364..897)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1067..1072,1073..1089,1090..1095))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1447..1538
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1463..1480
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1597..1614
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1627..1647
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2688..2822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2725..2744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2779..2798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2851..3016
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3200..3654
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3287..3306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3496..3517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3680..3784
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3785..3853,3854..4645)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4003..4022)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4816..5404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5305..5324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5558..5575
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcatagacca gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc
       61 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      121 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
      181 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
      241 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg
      301 gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa
      361 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
      421 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
      481 agcaccaagc gcgttggcct catgcttgag cagattgatg agcgcggtgg caatgccctg
      541 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
      601 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
      661 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
      721 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
      781 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
      841 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
      901 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
      961 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     1021 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
     1081 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     1141 aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt
     1201 ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct
     1261 gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc
     1321 ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg
     1381 tttgttcgcc caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat
     1441 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     1501 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     1561 ccaccatcgg gcgcggatct cggtccgaaa accatgcatc atcatcatca tcacagcagc
     1621 ggcagagaaa acttgtattt ccagggcggc atcccgaggc acgcccggca gatcaacaca
     1681 ctcagccacg gggaggtggt gtgtgccgtg accatcagca accccacgag gcacgtctac
     1741 acaggtggca agggctgcgt gaagatctgg gacatcagcc agccaggcag caagagcccc
     1801 atctcccagc tggactgcct gaacagggac aattacatcc gctcctgcaa gctgctccct
     1861 gatgggcgca cgctcatcgt gggcggcgag gccagcacgc tcaccatctg ggacctggcc
     1921 tcacccacgc cccgcatcaa ggccgagctg acgtcctcgg ctcccgcctg ttatgccctg
     1981 gccattagcc ctgacgccaa agtctgcttc tcctgctgca gcgatgggaa cattgctgtc
     2041 tgggacctgc acaaccagac cctggtcagg cagttccagg gccacacaga tggggccagc
     2101 tgcatagaca tctcccatga tggcaccaaa ctgtggacag ggggcctgga caacacagtg
     2161 cgctcctggg acctgcggga gggccgacag ctacagcagc atgacttcac ttcccagatc
     2221 ttctcgctgg gctactgccc cactggggag tggctggctg tgggcatgga gagcagcaac
     2281 gtggaggtgc tgcaccacac caagcctgac aagtaccagc tgcacctaca cgagagctgc
     2341 gtgctctccc tcaagttcgc ctactgcggc aagtggttcg tgagcactgg gaaagataac
     2401 cttctcaacg cctggaggac gccttatgga gccagcatat tccagtctaa agaatcctcg
     2461 tctgtcttga gttgtgacat ttcagcggat gacaaataca ttgtaacagg ctctggtgac
     2521 aagaaggcca cagtttatga ggtcatctac tgatgacgaa gcttgtcgag aagtactaga
     2581 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2641 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2821 tctgatcact gatatcgcct aggagatccg aaccagataa gtgaaatcta gttccaaact
     2881 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     2941 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     3001 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
     3061 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
     3121 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
     3181 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3241 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3301 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3361 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3421 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3481 tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3541 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3601 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3781 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3841 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3901 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3961 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4021 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4081 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4141 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4201 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4261 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4321 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4381 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4441 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4501 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4561 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4621 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4681 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4921 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5461 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5521 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5581 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacacc
//
