LOCUS       sequence-42622-e        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, 
            Howarth M
  TITLE     Peptide tag forming a rapid covalent bond to a protein, through 
            engineering a bacterial adhesin.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 
            24.
  PUBMED    22366317
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-20"; volume: "109(12)"; pages: 
            "E690-7. Epub 2012 Feb 24"
COMMENT     Sequence Label: pDEST14-SpyCatcher EQ
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             122..460
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVQTAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             525..542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(595..613)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      609..656
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      693..1045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(780..799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             1225..1234
                     /estimated_length=10
ORIGIN
        1 annnnnntnn nnnntttgtt tactttaaga aggagatata catatgtcgt actaccatca
       61 ccatcaccat cacgattacg acatcccaac gaccgaaaac ctgtattttc agggcgccat
      121 ggttgatacc ttatcaggtt tatcaagtga gcaaggtcag tccggtgata tgacaattga
      181 agaagatagt gctacccata ttaaattctc aaaacgtgat gaggacggca aagagttagc
      241 tggtgcaact atggagttgc gtgattcatc tggtaaaact attagtacat ggatttcaga
      301 tggacaagtg aaagatttct acctgtatcc aggaaaatat acatttgtcc aaaccgcagc
      361 accagacggt tatgaggtag caactgctat tacctttaca gttaatgagc aaggtcaggt
      421 tactgtaaat ggcaaagcaa ctaaaggtga cgctcatatt taaatggttg atgcttgagg
      481 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      541 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      601 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      661 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      841 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      901 acttgattag ggtgatggtt cacgtagtgn ncatcgccct gatagacggt tttcgccctt
      961 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaactggaa cacactcaac
     1021 cctatctcgg tctatctttg atttatnagg atttgccgat ttcggcctan tnnnaaaatg
     1081 agctgantna nnaaaanttn nncnnntnnn naanntnngn tnnnnatttn gngnnntttc
     1141 gnnnnnnnnc gnnccnantn nnnnttncna nnnnntcann nnnnnccnnn nnnnnnannn
     1201 nnnnnangcn ncannnnntn aaaannnnnn nnnngnnnt
//
