LOCUS       sequence-426226-        9362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid for expression FAST-PETase in Pseudomonas putida.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Lu H, Diaz DJ, Czarnecki NJ, Zhu C, Kim W, Shroff R, Acosta DJ, 
            Alexander BR, Cole HO, Zhang Y, Lynd NA, Ellington AD, Alper HS
  TITLE     Machine learning-aided engineering of hydrolases for PET 
            depolymerization.
  JOURNAL   Nature. 2022 Apr;604(7907):662-667. doi: 10.1038/s41586-022-04599-z.
            Epub 2022 Apr 27.
  PUBMED    35478237
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04599-z"; journalName: "Nature"; date: 
            "2022-04"; volume: "604"; issue: "7907"; pages: "662-667"
COMMENT     Sequence Label: pBTK522-FAST-PETase
FEATURES             Location/Qualifiers
     source          1..9362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             412..1251
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             1238..2089
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIALAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     complement(2237..2256)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2248..3063
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2248..3063
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2321..2340)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             3099..4181
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3118..3137)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      4201..4249
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(4547..4564)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    5449..5473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      6230..6624
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            6966..7053
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8292..9263
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc aaacaaggcc
       61 gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg ctgcaccttg
      121 tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga ctgatatgaa
      181 agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg tacgccaagc
      241 gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct ggctgaccga
      301 cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc agggcggggg
      361 tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc aatggctacc
      421 cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct ggactacgtt
      481 ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg tgccggtaaa
      541 tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct gctggaggtg
      601 ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc caccgccatt
      661 catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca agccgtggct
      721 gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc cccggagtgg
      781 ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga cacgctgcgc
      841 cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat cggtcgcatg
      901 gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc cagcaagggc
      961 gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc ggtactggtc
     1021 gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc cgaggaatgg
     1081 ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa ggccaactat
     1141 ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct caagcccgcc
     1201 gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta agaacaagca
     1261 cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc tgttccgtgc
     1321 cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact acggcgacgg
     1381 caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc gcatcctgca
     1441 agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac ccaagaccga
     1501 aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca ccgctgatgc
     1561 catggtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag aggtcgatag
     1621 tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg tatccatcat
     1681 cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg ccagcgacga
     1741 ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgctggccg tcatgggtgg
     1801 cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg aaaccgcccg
     1861 cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca aggcttccat
     1921 agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca tgcgcaagcg
     1981 ccgccagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga cggtaaccga
     2041 gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac tcacgttaac
     2101 tgatccttca actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat
     2161 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     2221 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcc
     2281 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2341 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
     2401 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2461 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2521 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa
     2581 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2641 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg cctcgctcag
     2701 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg atttcgatga cgagcgtaat
     2761 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
     2821 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2881 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2941 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3001 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3061 taatcagaat tggttaattg gttgtaacac aggtactcat gaaaccagta acgttatacg
     3121 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     3181 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     3241 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     3301 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     3361 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     3421 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     3481 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     3541 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     3601 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     3661 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     3721 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     3781 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     3841 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     3901 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     3961 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     4021 ctcagggcca ggcggtgaag ggcaatcagc tgttgccggt gtcactggtg aaaagaaaaa
     4081 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4141 agctggcacg acaggtttcc cgactggaaa gcgggcagtg atgtaaaagc aagctgataa
     4201 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacatgaaga
     4261 aattattatt tgatgatcag atagcggcgg ggaactgcca gacatcaaat aaaacaaaag
     4321 gctcagtcgg aagactgggc cttttgtttt atctgttgtt tgtcggtgaa cactctcccg
     4381 gttgtaccct ccatcgcctc gcattctcgg atgtcctggg ggttcgtaac atctctgtaa
     4441 ctgcttgaga cgcagctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc
     4501 cgaagcgggt ttttacgtta tttgcggatt aacgattacg gattcagtgg tggtggtggt
     4561 ggtgctcgag gctgcaattc gctgtacgaa aatcagacac tgcggtgcta ttcgggttct
     4621 cgcaggcaaa agtagaatac ctagtatcat tgtccataaa acgtttcatc catgcaacgc
     4681 ctttttttcc gattaatgct tggttagaat tgccgctatt cgcacaggaa tgtgagccac
     4741 ccttaatttc gagaaactgc ttagcgttct gtgacataga atcatagata ggaagtgctg
     4801 aagagttgac cggggctata ctatcgtttt cacatgcgaa gatcagcgtg ggcacagtta
     4861 cagaactaaa atttgtcgaa gagtgccatg gtgcttgagg cgccgctgct ttcagcgaag
     4921 ggttgttagc agcagagatc agggagcctc cacctcccat actccacccc ataacaccca
     4981 tacgagcggt atcgaccttt ccgtaaatcg ggctgctact tgtaccgttt aacgacgcca
     5041 cctggcgcag cgcggccatt tgttgcgaac tccgactttc tggctggtct aacgtggaat
     5101 ttgtgtcaat tgtaattact acgaaaccgt gggatgccag acgcggtccc caccatttaa
     5161 ttgatgactg ccgtgccgta tacccgggaa ctatggctat ggcgcccacg gtacccccag
     5221 catttgttgg atagtaaacg gtgccagcgc cataaccgct cggtctactc acggtaaagg
     5281 agcgaacggt gaatggacca gcgctcgctt ccaaactggc ggctgtcgga ttcggaccgc
     5341 gcgcatacgg attggtctgg gccatggacg gcaacggcaa cagcatcgcc gccaatacgg
     5401 cggctcgcag gatgtggctc atatgtaata tctcctctgt ttctgtatgg aattgttatc
     5461 cgctcacaat tccaacagta ctatgtgatt ataccagccc cctcactaca tgtcaagaat
     5521 aaactgccaa aggacagtca ttcatctttc tgcccctcca aaagcaaaaa cccgccgaag
     5581 cgggttttta cgtaaatcag gtgaaactga ccgacgttgt attgcgacga attgccacgt
     5641 tgtcgcagtg tctgtactgt gggttaatgt ctgtgatcgc cgtcgtgagt tcgtgtcatc
     5701 cgcgcatgat tcagatggtc ctggagatcg ttgcttgtac cggctgtttg tggctctacc
     5761 acgtctcatc agtctagatg cgaattcagg ggcctgtcag accaagttta ctcatatata
     5821 ctttagattg atttctgaaa gcgaccaggt gctcggcgtg gcaagactcg cagcgaaccc
     5881 gtagaaagcc atgctccagc cgcccgcatt ggagaaattc ttcaaattcc cgttgcacat
     5941 agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt
     6001 caacgatctg atagagaagg gtttgctcgg gtcggtggct ctggtaacga ccagtatccc
     6061 gatcccggct ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt
     6121 gtttacatac agtctatcgc ttagcggaaa gttcttttac cctcagccga aatgcctgcc
     6181 gttgctagac attgccagcc agtgcccgtc actcccgtac taactgtcac gaacccctgc
     6241 aataactgtc acgcccccct gcaataactg tcacgaaccc ctgcaataac tgtcacgccc
     6301 ccaaacctgc aaacccagca ggggcggggg ctggcggggt gttggaaaaa tccatccatg
     6361 attatctaag aataatccac taggcgcggt tatcagcgcc cttgtggggc gctgctgccc
     6421 ttgcccaata tgcccggcca gaggccggat agctggtcta ttcgctgcgc taggctacac
     6481 accgccccac cgctgcgcgg cagggggaaa ggcgggcaaa gcccgctaaa ccccacacca
     6541 aaccccgcag aaatacgctg gagcgctttt agccgcttta gcggcctttc cccctacccg
     6601 aagggtgggg gcgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat tcagcccggc
     6661 tcatccttct ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac
     6721 gcatccattg ccgccatgag ccgatcctcc ggccactcgc tgctgttcac cttggccaaa
     6781 atcatggccc ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc
     6841 cttgcccgca cccgctgaat ttcggcattg attcgcgctc gttgttcttc gagcttggcc
     6901 agccgatccg ccgccttgtt gctcccctta accatcttga caccccattg ttaatgtgct
     6961 gtctcgtagg ctatcatgga ggcacagcgg cggcaatccc gaccctactt tgtaggggag
     7021 ggcgcactta ccggtttctc ttcgagaaac tggcctaacg gccacccttc gggcggtgcg
     7081 ctctccgagg gccattgcat ggagccgaaa agcaaaagca acagcgaggc agcatggcga
     7141 tttatcacct tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc agggccaagg
     7201 ccgactacat ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg
     7261 aatccgggca catgccggag ttcgtcgagc ggcccgccga ctactgggat gctgccgacc
     7321 tgtatgaacg cgccaatggg cggctgttca aggaggtcga atttgccctg ccggtcgagc
     7381 tgaccctcga ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg accggtgccg
     7441 agcgcctgcc gtatacgctg gccatccatg ccggtggcgg cgagaacccg cactgccacc
     7501 tgatgatctc cgagcggatc aatgacggca tcgagcggcc cgccgctcag tggttcaagc
     7561 ggtacaacgg caagaccccg gagaagggcg gggcacagaa gaccgaagcg ctcaagccca
     7621 aggcatggct tgagcagacc cgcgaggcat gggccgacca tgccaaccgg gcattagagc
     7681 gggctggcca cgacgcccgc attgaccaca gaacacttga ggcgcagggc atcgagcgcc
     7741 tgcccggtgt tcacctgggg ccgaacgtgg tggagatgga aggccggggc atccgcaccg
     7801 accgggcaga cgtggccctg aacatcgaca ccgccaacgc ccagatcatc gacttacagg
     7861 aataccggga ggcaatagac catgaacgca atcgacagag tgaagaaatc cagaggcatc
     7921 aacgagttag cggagcagat cgaaccgctg gcccagagca tggcgacact ggccgacgaa
     7981 gcccggcagg tcatgagcca gacccagcag gccagcgagg cgcaggcggc ggagtggctg
     8041 aaagcccagc gccagacagg ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta
     8101 gccgccgagg tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg
     8161 aagctatggc taaccgtgat gctggcttcc atgatgccta cggtggtgct gctgatcgca
     8221 tcgttgctct tgctcgacct gacgccactg acaaccgagg acggctcgat ctggctgcgc
     8281 ttggtggccc gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca
     8341 tgggctgtga gcgcttcgat atcggcgtca gggacgccac caccggccag atgatgaacc
     8401 gggaatggtc agccgccgaa gtgctccaga acacgccatg gctcaagcgg atgaatgccc
     8461 agggcaatga cgtgtatatc aggcccgccg agcaggagcg gcatggtctg gtgctggtgg
     8521 acgacctcag cgagtttgac ctggatgaca tgaaagccga gggccgggag cctgccctgg
     8581 tagtggaaac cagcccgaag aactatcagg catgggtcaa ggtggccgac gccgcaggcg
     8641 gtgaacttcg ggggcagatt gcccggacgc tggccagcga gtacgacgcc gacccggcca
     8701 gcgccgacag ccgccactat ggccgcttgg cgggcttcac caaccgcaag gacaagcaca
     8761 ccacccgcgc cggttatcag ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca
     8821 ccgctggccc ggcgctggtg cagcaggctg gccagcagat cgagcaggcc cagcggcagc
     8881 aggagaaggc ccgcaggctg gccagcctcg aactgcccga gcggcagctt agccgccacc
     8941 ggcgcacggc gctggacgag taccgcagcg agatggccgg gctggtcaag cgcttcggtg
     9001 atgacctcag caagtgcgac tttatcgccg cgcagaagct ggccagccgg ggccgcagtg
     9061 ccgaggaaat cggcaaggcc atggccgagg ccagcccagc gctggcagag cgcaagcccg
     9121 gccacgaagc ggattacatc gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc
     9181 agcttgcgcg ggccgagctg gcacgggcac cggcaccccg ccagcgaggc atggacaggg
     9241 gcgggccaga tttcagcatg tagtgcttgc gttggtactc acgcctgtta tactatgagt
     9301 actcacgcac agaagggggt tttatggaat acgaaaaaag cgcttcaggg tcggtctacc
     9361 tg
//
