LOCUS       sequence-426227-       24970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector expressing the estradiol-inducible Lineage Tracing 
            system. XVE expression driven by M. polymorpha YUC2 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24970)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 24970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: ipYUC2Cas-LT1
FEATURES             Location/Qualifiers
     source          1..24970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    214..238
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     291..313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5986..6237
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             6253..6486
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             6496..7431
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6544..7437
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        8235..8281
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             8303..12406
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8303..12406
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12419..12439
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        13406..13481
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(15704..15706,15707..15709,15710..16420)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(15749..15770)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     16357..16378
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             17789..18817
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      18825..19532
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(19565..19583)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(19566..19583)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    19596..19643
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    19755..19779
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      19859..20490
                     /label=oriV
                     /note="incP origin of replication"
     CDS             21901..22695
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             22994..24142
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(24382..24970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(24462..24481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt ggatctcctg
      121 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
      181 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
      241 cactgatagt ttaaactgaa ggcgggaaac gacaatctgc tagtggatct cccagtcacg
      301 acgttgtaaa acgggcgccc cgcggaaagc ttgctagcca attggggccc aacgttctcg
      361 agtttttcta gaaggccttg gatccacccg ggagaattcg tcgactttgc ggccgcatcg
      421 atactgcagg agctcggtac cttttactag tgatatcgat catgagcgac ctcgtcacca
      481 catgaacctc acctcctcat ttaaaatagt ataatgctgg attgattgaa gaattagaca
      541 taattctttt gcgcatccct ttatgttttg ttttcatttt ttgggcgatg tcagttaacg
      601 cggtggtatt gcatggcggc tacatgcaag tacattcata gtttaactgg agcgaggcct
      661 ttcacacgtg tcctcccttt aaaacaacgg ctttgaaagt aagtccgtgt cgccacacaa
      721 ttcggctttc ccagcccaag cctcgaatca tgaggactac gaacagacac acacagtcca
      781 gcgagcgcga tcgagccttc tcgctctctc tctctcccct ctcgctggct cttctgccct
      841 ggggccgatg gaacgaacga acgagcccac ctttctcctc tctctctctc tctctctctc
      901 tcctcgctca ctggccacgt cagtggatgc agaggctggg ggcggctgga atcgctcgat
      961 cgaccaccgg ccgtcaccct cgctacgaac cccgccggcc cgaccctggc cccgtggttc
     1021 cgctctctcg tcgacccggt ccacggcccc ggtcccgttg tgaaatgaat caatccgctc
     1081 gcccgctgcc ccgaggtcag tcaggtctgg tacgggcctg gtattctcgg cgatgtactc
     1141 tgactggcag tcgttcgttt gtgggccgcg gggggatata ctggagagag aaagagatgg
     1201 agagagggag agagagggag ggatgggaga gagagagaga ggtcaaagag agccttttgg
     1261 ttggagtaaa tgagattgtc cgttttccgt tacaacagca ggcggagagg gatcgagaga
     1321 gcatcattga gatgaggtgc cgggacggag tgtagtgaat tgaatcgact cgagtcgaat
     1381 tgaatcgatg gcattgattc gagggcgctt gggatgattc gctgccgttg ggggttgggg
     1441 ttcacgtagt gctctcgaac tggaaaccag tccgcaacag tgtctcacat attccccact
     1501 aggcgcgacg atggatggag aaaaatgcga ggggcccggc cccaggcgca ggactacaca
     1561 cgtccagttg tcttgtctcg gcagacattt gctcactcag tgatggagag cctgtaacgt
     1621 cagggctcct gatgtcacgc ctgcccgcat cgcatgagac aaaggcggga gtcctcacgc
     1681 gcatcttttc gcccctccga tctcagtcgc ctttggctca gctccccgcc cctaatttcc
     1741 attaccaact aaccacgaag tcgggcccgg gccgcgggcc cagatccata gatcctccct
     1801 cccttccatg tggattctcc gagccaatat acagtttggc aatatatatg tctgtatttc
     1861 aaagagggcc gcacatgaga ggccaaaatt ggttaaaata cagccttttg attccccagc
     1921 tccagagcca tcgagtcagg ttgcacatgg gtctttcgag gtccgtgcac ggaggtgatc
     1981 cgcctctgag atcagtcatt gtacagcctc ggacctccag cgcgagagag tgcgaagtgg
     2041 ccgcgagcag tagcagtagc agcagcagca gcggcgtagg agtacgagga tagggatgac
     2101 attcgatcgg tttcgctaac ggaacgggac gaaaagaagg gacgagatcg acaccaaaag
     2161 caatccaaag caaaggaatg gaaagcgaga agaagaaggc cgcccacttc tttcgctttc
     2221 gaggcctcct cgtcctctcg tctgcaccac caccgcagcg agcgaggtcc ttcctccctt
     2281 ggccctcctc gagcctacgt ttggcccagc cccgtaggcc aaacgaaaga actatccatc
     2341 catccttcct tccttcccat ccatccccgt tggcactcag gcaggcaggc aggcaggcaa
     2401 aagcggcagc tacggcctct tgcatgaaag aatgtatcat cttttgccag tcgcaggtcg
     2461 tcggcctcca ccgccggccc tccttcctct ccgggcagcg ccggaaagct agagtttgac
     2521 gcgcccgtgg cccgcctcgc cggtgatgcc caaggcgccg cgcatatccg aacgtttcgg
     2581 ttgcgacgga ccgtccatcg ggatggggct caataaagaa ggcagcaaag gaaggaaagg
     2641 aatggaaagg aaaggaaagg aagggaaggt gcggtggtac agcgtaatgt gctcggccga
     2701 gaggtatcgg caacagtaaa agagaatgtt tcagaacact gtgggaaaag gcacgaagga
     2761 aggaaggaag gaggcatggg atgggatggc atgaggcatg gggcatgggg catggctggg
     2821 ctctcatgcg ttcgtcgcgc ctgcagcaga tagagctcgc agagcgtcgc caccgggaca
     2881 cacgaacgag cgcacatccg gaaaagcctt ctccagggat tcatgatcgg ggctcttgga
     2941 cgaccttcgc agcaatgctc ggacccaatc acccggagct ctgatcctcc ccgcccctcg
     3001 cctcgccggg atgcgtcgcc tctcgctttc tgtcacggct ggcactgcag ggcttgcttt
     3061 ttcgcagtgt gcttttctgg cgggcctcct ttttctgtaa gcctcttccg acagagggag
     3121 ggagcggaga cgaaaagaag aagcaacgag cggaagatac tgtacgtgtc ctcgttctcc
     3181 ctcggacgtc ccgtcaccat cgagcgagga ggacaaacgg gcgaaccggt tgacatccgc
     3241 aaccttgtcc gggccgcggg cgagctcgca gcgatgcggg cgtttggtgc cgacattcat
     3301 ccttattaat cctcgacgca gccgcgaatg ccatcgacca caagacgtta agcgaaaatg
     3361 atgcgaacgc aattcttgca agatatcgtt ttgcaggccg aattaaagcc gttggaatga
     3421 tgacttaaat gccccctttt attagtagaa agtcaggttg gcggaattat gaggtaggtg
     3481 gtgactgggc gatcggcgaa ggattgcaag ttgatttcgt ttgatgcgag cggattcatt
     3541 cggtgagaat aagctgcgca tttggaagtg gctgcacatg ataccgacga tttgaggcag
     3601 acgggcgaac ggggatcagg cgaatgagca tgagtgcagc agccctcgcc ctccactcca
     3661 ctccactcca cttcactccc gctttggaac ggtcatcaat taagcgatac tgctcgagcc
     3721 caaatctgcc gacgagccgg atttctaaat catgatttcc cgagatgagt gagaaaatta
     3781 tcaacctcta tgggaatgat cacagctaag ctaatcgcta gactagacgt ccgagggaat
     3841 tgatcattta tttcgcgtgc aattttataa gtttacttga gtggtgcctc tggttcgaaa
     3901 accataaaaa gaaaggaggg aaacatttgc cagcttaaaa ggtaccacat ttggccccgg
     3961 tgtacgattc ttgttgaaga atacctcgga cgggttctgt tcggttctgt tccaagatac
     4021 gatatggagc cgctccaaaa gcctgccatg attgttcaag ttggagctag gagaaaagcg
     4081 ttgctactag tctaaaggcc tcaaccaact atcacccaaa ccccgattca ttgcgtttct
     4141 aaaggattac tttcccaaaa gaaaaccccg gaaatcacat ccttcctttc cggattgaaa
     4201 tatacaaact cccccctccc cctccccacc cccacatgtc ctgcccatcg gactccagcg
     4261 ggcaagcacg cttgagcatc ctcgaggccc tatcccatcg cgacgacgcc cctcctctcc
     4321 cctgctcatc gagagctcgg agcccaaggg cgtgtcggag agaggcccgc aaaattcgcc
     4381 ctcaaaattc gcccgaaaac gaagctctgc tctcttccct gctgtctgtg gtctgctctc
     4441 ttctctgctt tctatggcgc cccgataagg agcgagggcg gcccgctcga tcggtgaccg
     4501 cccttcggtc gcaacgcaaa gtccagaaat gccccaaata cgtggacctt gggggcgagc
     4561 cgctcaggta gagttaaaat ataatttttc aggggaatga aatgaggacc aggtgtcgac
     4621 atatcagtgg gcttatattc aagatgttct ggcctaaaac cttgaggtat aaaggcgagg
     4681 acaagagttt ttgtccacga taagatttct ctgacattcc gatatccctt ttcggcccct
     4741 aataacctgc gctgtcaccg ggaaagcggg ccacaatttc ccggccggaa acatgggcca
     4801 aacacctccc tccctccctc cctccctccc gtcgccctgc gcgaccatcc ccggcaggca
     4861 agccgcgggc ctaggcctct agcggtactg taccccccag gcctagaacc gtaccctgag
     4921 tgcccagaga gaacagcaga ggcctgcagg tcaggtcagg tcaggtcagg gcaggcaatg
     4981 gcagcgaagg gcaggagcgg accctttcgg gtttggaccg ctggatgcga aactgccggg
     5041 aacgtccgct gccgagggag aaagtgaaaa tccggcgccg ggctcgctcg ggctgaaata
     5101 cgtgtgccgt ggtaatcatc aagaccatca gcacgcagca gtgtcgagcc aaacgctctg
     5161 cgcttccccc acccccgccc attcaagtag acgccgctct cgtcccgttt aggcctattc
     5221 caccggactg ggctcgcatc ctctccttcg ctcgatcccc tcccctcccc acctctgggc
     5281 caaaaccaca aagccttcag accctccgcc ctcagacttc gctgctccac ctccactact
     5341 acatcgctct cgtcgacctc gtcctcgcat tccgctcgct tccaatcctt tagggctcga
     5401 gccctcccat cctggaggga cttcctgtac tctcctctct cttctttgcc aagcgccacc
     5461 tttttctccc gtgtacttgc gacctcctcc tcgcattcag cctccggacg ttctgttctc
     5521 ttcttcacgc cttgcgcctc gtgtctttcg taaattctct ccgaccgacc agcgcggtgt
     5581 ttgcttatcc gtctttcccg actctctctc gcaggctctt gtttagaatc tgactgctcg
     5641 tcatttgcgg tttgcgcttc tggccccgga gcgtcgtcgt acacagctta tgctccgtgc
     5701 aaatctgtgg tgaaatctgg agaagatctg cacccgaatt ttctcgctta attgtccgtc
     5761 gggctgctgt gggtcgagcg cagcgagtag tgcgtcctct gtagagctgt accgggcgga
     5821 aaatgtgctc cacgattcac attgatttgt gatatataaa gtcagcgttg tgcgggcttg
     5881 cggctgtaat tgtgtgattg gagcctctga gattggcaac gttgagagtc ttcaggagaa
     5941 gaggcggacc cgaagacatc aatttgctga tttcgtcgac aaacaatgaa agcgttaacg
     6001 gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg
     6061 ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa
     6121 catctgaagg cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg
     6181 attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt
     6241 gaaccgtcga gcgccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc
     6301 gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg
     6361 gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta cggcgctctg
     6421 gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac
     6481 ggtggggatc cgtctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
     6541 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
     6601 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
     6661 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
     6721 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
     6781 cttctagaat gtgcctggct agagatcctg atgattggac ttgtctggcg ctccatggag
     6841 cacccaggga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
     6901 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
     6961 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
     7021 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
     7081 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
     7141 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
     7201 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
     7261 gacctgctgc tggagatgct ggacgcccac cgcctacatg cgcccactag ccgtggaggg
     7321 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
     7381 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agtctgaggc
     7441 tgagctccct ggcggaattc ccagagatgt tagctgaaat catcactaat cagataccaa
     7501 aatattcaaa tggaaatatc aaaaagcttc tgtttcatca aaaatgactc gacctaactg
     7561 agtaagctag cttgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
     7621 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
     7681 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
     7741 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
     7801 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
     7861 gtaaaacatc ccaaacaggc tcctagcaaa gtcgacgaat tctcccgggt ggatccaagg
     7921 ccttctagaa aaacgaattc catgccagct tgggctgcag gtcgaggcta aaaaactaat
     7981 cgcattatca tcccctcgac gtactgtaca tataaccact ggttttatat acagcagtac
     8041 tgtacatata accactggtt ttatatacag cagtcgacgt actgtacata taaccactgg
     8101 ttttatatac agcagtactg tacatataac cactggtttt atatacagca gtcgaggtaa
     8161 gattagatat ggatatgtat atggatatgt atatggtggt aatgccatgt aatatgctcg
     8221 actctaggat cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     8281 acacgctgaa gctagtcgct gcatggataa gaagtactct atcggactcg atatcggaac
     8341 taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta agaagttcaa
     8401 ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg ctctcctctt
     8461 cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa gaaggtacac
     8521 cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga tggctaaagt
     8581 ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag ataagaagca
     8641 cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg agaagtaccc
     8701 tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg atctcaggct
     8761 catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca tcgagggtga
     8821 tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc agacctacaa
     8881 ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc
     8941 tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc ctggtgagaa
     9001 gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc ctaacttcaa
     9061 gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata cctacgatga
     9121 tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt tcctcgctgc
     9181 taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca ccgagatcac
     9241 caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc aggatctcac
     9301 acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga ttttcttcga
     9361 tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag aagagttcta
     9421 caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc tcgtgaagct
     9481 caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta tccctcatca
     9541 gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct acccattcct
     9601 caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt actacgtggg
     9661 acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg aggaaaccat
     9721 caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt ctttcatcga
     9781 gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta agcactcttt
     9841 gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg tgaccgaggg
     9901 aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt
     9961 caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca agaaaatcga
    10021 gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat ctctcggaac
    10081 ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg aggaaaacga
    10141 ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag agatgattga
    10201 agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc agttgaagag
    10261 aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa tcagggataa
    10321 gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta acagaaactt
    10381 catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga aggctcaggt
    10441 ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat ctcctgcaat
    10501 caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag
    10561 gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca ctcagaaggg
    10621 acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag agcttggatc
    10681 tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga agctctacct
    10741 ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata tcaacaggct
    10801 ctctgattac gatgttgatc atatcgtgcc acagtcattc ttgaaggatg attctatcga
    10861 taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg tgccaagtga
    10921 agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta agctcatcac
    10981 tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg aattggataa
    11041 ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc acgttgcaca
    11101 gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga tcagggaagt
    11161 gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt tccaattcta
    11221 caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt
    11281 tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt acggtgatta
    11341 caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg gaaaggctac
    11401 cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga ttaccctcgc
    11461 taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag gtgagatcgt
    11521 gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa
    11581 catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta tcctccctaa
    11641 gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga aatacggtgg
    11701 tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg agaagggaaa
    11761 gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg aaaggtcatc
    11821 tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg ttaagaagga
    11881 tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta gaaagaggat
    11941 gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt
    12001 taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag aagataacga
    12061 gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca tcgagcagat
    12121 ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc
    12181 ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca tccatctctt
    12241 cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag
    12301 gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc agtctatcac
    12361 tggactctac gagactagga tcgatctctc acagctcggt ggtgattcaa gggctgatcc
    12421 taagaagaag aggaaggttt gagacgcagg cctcccagct ttcgtccgta tcatcggttt
    12481 cgacaacgtt cgtcaagttc aatgcatcag tttcattgcc cacacaccag aatcctacta
    12541 agtttgagta ttatggcatt ggaaaagctg ttttcttcta tcatttgttc tgcttgtaat
    12601 ttactgtgtt ctttcagttt ttgttttcgg acatcaaaat gcaaatggat ggataagagt
    12661 taataaatga tatggtcctt ttgttcattc tcaaattatt attatctgtt gtttttactt
    12721 taatgggttg aatttaagta agaaaggaac taacagtgtg atattaaggt gcaatgttag
    12781 acatataaaa cagtctttca cctctctttg gttatgtctt gaattggttt gtttcttcac
    12841 ttatctgtgt aatcaagttt actatgagtc tatgatcaag taattatgca atcaagttaa
    12901 gtacagtata ggctttcagc tttttttctt cttcttcgtt catacagttt ttttttgttt
    12961 atcagcttac attttcttga accgtagctt tcgttttctt ctttttaact ttccattcgg
    13021 agtttttgta tcttgtttca tagtttgtcc caggattaga atgattaggc atcgaacctt
    13081 caagaatttg attgaataaa acatcttcat tcttaagata tgaagataat cttcaaaagg
    13141 cccctgggaa tctgaaagaa gagaagcagg cccatttata tgggaaagaa caatagtatt
    13201 tcttatatag gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc gcttagataa
    13261 gaaaacgaag ctgagtttat atacagctag agtcgaagta gtgattggaa caaagcacca
    13321 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
    13381 gtgcagtccc ctccacccca cagtggtttt agagctagaa atagcaagtt aaaataaggc
    13441 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttttt ttttctgcca
    13501 aatgagtcac acacattgtt gagagacata tcaaaataac tcaattgtaa ggtaagttga
    13561 tagatttccc agttaaatgc attattttaa caatatttgt atctcaaatt ttcaaaatca
    13621 tatcaaattt gcagatcttg atgatgattc acaactctgt cttcgatttt tgattgttaa
    13681 cgggtatcga tatttgggta acttaaaaag aatttgctaa agatcttaaa aaacagatta
    13741 aaaaaacctt tcaaaatagc taatccttta aatccgtttc acaatataat gctcattgat
    13801 gataatcgaa aagattccat tttaaagtgc atataaattg cttaattatc tctttctgtc
    13861 tcgtaatatt ttgttctctc ctattggatc ggacccgcat cttctcgatc agttcactat
    13921 actaatattt ggtcaacttc aagagagatt agaacttcgt tctccaaagt gtaaaggccg
    13981 acaagcagaa aaggaaactt caatcagaag ccttctaggt tttcccgcgt cccaaaaagc
    14041 gaagggagga cggggaggac ggtgaggcca gaagaagaaa gcaacagtaa aatctcatgt
    14101 gattgcgtgc atgtcatgga tctatgggat gctagtccac cgtttggaag tatgtgcaga
    14161 aggagaaagg cgcgggaggc caaaagcgac ttcggccacc tctctacttt ctagaaagtt
    14221 tgctcgcagc ttgtaataag cgcggaaaag gtactcgaaa aaccggagca atcggtgtgg
    14281 tatcagcagt cgggttcgct aaacctacgt cttcttattt tggaatgtta aattgaaagg
    14341 gcgtgccggc cctaagcacg gacgatgtga cagacggagg agtgtgagca attcggatga
    14401 cgatgctggc cggcagtcac gacaaagagt ccgaaggggc atggacaggg caatatataa
    14461 atggaaaaga ggctttgaat attcctaacc cttcgtcgtc ggcctcccaa cttgccctcg
    14521 catgctgaat tggccgccta tctgcgaatg gccggccgcc gaccctaagt cgcaataact
    14581 tgaacggagc cgatgcaaag tccttatcga acgctcgtta ttataaacgg ggcagaccta
    14641 ggtatttaat tatatcaaaa ccctccgcaa tttcgctaat cattccctaa tttcttctac
    14701 tttggcgtct agtgctgtgg tcgcgcttgc tctcaaagct cgctgggagg cagtggagcg
    14761 tctggcttaa aatcgatcgc cgcaacccta gagttccttt cacttctttc tctcttgcgt
    14821 ggagagcatc gacatcgagg agctgctact tgtcttctgg tatgcgcctt gtgccctcgt
    14881 ctctgtacgt cgttacgatt gctcgatatt gtgattgtct agacctgaaa cccgactgga
    14941 gcttttacgt ggtgtcgatt ggggcctcga gcgagtgatt ttttaggttc gttttagcca
    15001 agtacatccc actttggtca gtcctgttat ctcggtaagg tgttcagttc ggtcactggt
    15061 gcggaggtgc ctcgtaagtt tcctgcatga tagatttgtc tatccttgac ttgaagagga
    15121 gtcgaaggtc agcgaggttg gctgtgtgct atggacgtcg gtgctccttg gttccgtgct
    15181 tctatgggtg ttgcctcaac agtattgatg tgcctgcaga aaggttgtca ccgacgatgg
    15241 tcccctccac cccacagtgg gggcaccaag agccgagaag aagcccgccg agaaaaaaac
    15301 cgccgctgag aggccggtgg aagagaacaa ggctgctgaa aaggctccgg cggagaagaa
    15361 acccaaggcg gggaagaaat tgccaccaaa ggaagccggt gacaagaaga agaagcgatc
    15421 taagaaaaac gtcgaaacct acaagatcta catcttcaag gtgctgaagc aggttcatcc
    15481 agatatcgga atttcaagca aagccatggg gattatgaac agtttcatca acgacatatt
    15541 cgagaagcta gctcaggaat cttcgaaact cgctaggtac aacaagaagc cgaccattac
    15601 atctcgcgag atccagacgg ctgtgagact cgtcctcccc ggtgaactcg ctaaacacgc
    15661 cgtctctgaa ggtaccaagg cggttaccaa gtttactagc tccatggtga gcaagggcga
    15721 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
    15781 caagttcagc gtccgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa
    15841 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
    15901 ctacggcgtg gcctgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
    15961 gtccgccatg cccgaaggct acgtccagga gcgcaccatc tctttcaagg acgacggtac
    16021 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
    16081 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt
    16141 caacagccac aacgtctata tcacggccga caagcagaag aacggcatca aggctaactt
    16201 caagatccgc cacaacgttg aggacggcag cgtgcagctc gccgaccact accagcagaa
    16261 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc
    16321 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    16381 cgccgccggg attacacatg gcatggacga gctgtacaag taatcaggtg gatcctagat
    16441 aacctttacc ttcattccct gtaaattggt accctgcatc tcgtctctgt tatgcttaag
    16501 aagttcaatg tttcgtttca tgtaaaactt tggtggtttg tgttttgggg ccttgtataa
    16561 tccctgatga ataagtgttc tactatgttt ccgttcctgt tatctctttc tttctaatga
    16621 caagtcgaac ttcttcttta tcatcgcttc gtttttatta tctgtgcttc ttttgtttaa
    16681 tacgcctgca aagtgactcg actctgttta gtgcagttct gcgaaacttg taaatagtcc
    16741 aattgttggc ctctagtaat agatgtagcg aaagtgttga gctgttgggt tctaaggatg
    16801 gcttgaacat gttaatcttt taggttctga gtatgatgaa cattcgttgt tgctaagaaa
    16861 tgcctgtaat gtcccacaaa tgtagaaaat ggttcgtacc tttgtccaag cattgatatg
    16921 tctgatgaga ggaaactgca agatactgag cttggtttaa cgaaggagag gcagtttctt
    16981 ccttccaaag catttcattt gacaatgcct tgatcatctt aagtagagtt tctgttgtgg
    17041 aaagtttgaa actttgaaga aacgactctc aagtaaattg atgatcacaa gtgaaagtgt
    17101 atgttactaa ctagtcgacg agtcagtaat aaacggcgtc aaagtggttg cagccggcac
    17161 acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc
    17221 aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt
    17281 agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc
    17341 ttcttctata aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct
    17401 caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt
    17461 ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct
    17521 ttggtttaga ttctgttaat cttagatcga agacgatttt ctgggtttga tcgttagata
    17581 tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag
    17641 ttttgtcgaa taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta
    17701 gtttgtgcga tcgaatttgt agattaatct gagtttttct gattaacacc acaccaatct
    17761 ggtaccagaa aaggggagaa gcaaaaccat gggtaaaaag cctgaactca ccgcgacgtc
    17821 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
    17881 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
    17941 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
    18001 cgcgctcccg attccggaag tgcttgacat tggggcattc agcgagagcc tgacctattg
    18061 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
    18121 tgttctgcaa ccggtcgcgg aggcgatgga tgccatcgct gcggccgatc ttagccagac
    18181 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
    18241 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
    18301 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
    18361 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
    18421 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
    18481 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
    18541 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
    18601 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
    18661 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
    18721 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
    18781 ccgacgcccc agcactcgtc cgagggcaaa ggaatagatg catcctgctt taatgagata
    18841 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
    18901 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
    18961 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc
    19021 tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag
    19081 cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa
    19141 ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca
    19201 aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
    19261 ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
    19321 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
    19381 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
    19441 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
    19501 gaattttgcg acaacatgtc gaggctcagc aggaccggca tgcaagctag cttactagtg
    19561 atattctata gtgtcaccta aatgtatgat ctagggaagt tcctattccg aagttcctat
    19621 tctctgaaaa gtataggaac ttctttgcgt attgggcgct cttggccttt ttggccaccg
    19681 gtcgtacggt taaaaccacc ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
    19741 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
    19801 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccactagaga
    19861 cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg
    19921 cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcag ccgccggcgt tgtggatacc
    19981 tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg
    20041 actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga
    20101 gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga
    20161 tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg
    20221 acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc
    20281 acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc
    20341 gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa
    20401 accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg
    20461 cccccccttc tcgaaccctc ccggcccgct ctcgcgttgg cagcatcacc cataattgtg
    20521 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
    20581 aagctgtttt ctggtattta aagttttaga atgcaaggaa cagtgaattg gagttcgtct
    20641 tgttataatt agggaaggtg cgaataagcg gggaaattct tctcggctga ctcagtcatt
    20701 tcatttcttc atgtttgagc cgattttttc tcccgtaaat gccttgaatc agcctattta
    20761 gaccgtttct tcgccattta aggcgttatc cccagttttt agtgagatct ctcccactga
    20821 cgtatcattt ggtccgcccg aaacaggttg gccagcgtga ataacatcgc cagttggtta
    20881 tcgtttttca gcaacccctt gtatctggct ttcacgaagc cgaactgtcg cttgatgatg
    20941 cgaaatgggt gctccaccct ggcccggatg ctggctttca tgtattcgat gttgatggcc
    21001 gttttgttct tgcgtggatg ctgtttcaag gttcttacct tgccggggcg ctcggcgatc
    21061 agccagtcca catccacctc ggccagctcc tcgcgctgtg gcgccccttg gtagccggca
    21121 tcggctgaga caaattgctc ctctccatgc agcagattac ccagctgatt gaggtcatgc
    21181 tcgttggccg cggtggtgac caggctgtgg gtcaggccac tcttggcatc gacaccaatg
    21241 tgggccttca tgccaaagtg ccactgattg cctttcttgg tctgatgcat ctccggatcg
    21301 cgttgctgct ctttgttctt ggtcgagctg ggtgcctcaa tgatggtggc atcgaccaag
    21361 gtgccttgag tcatcatgac gcctgcttcg gccagccagc gattgatggt cttgaacaat
    21421 tggcgggcca gttgatgctg ctccagcagg tggcggaaat tcatgatggt ggtgcggtcc
    21481 ggcaaggcgc tatccaggga taaccgggca aacagacgca tggaggcgat ttcgtacaga
    21541 gcatcttcca tcgcgccatc gctcaggttg taccaatgct gcatgcagtg aatgcgtagc
    21601 atggtttcca gcggataagg tcgccggcca ttaccagcct tggggtaaaa cggctcgatg
    21661 acttccacca tgttttgcca tggcagaatc tgctccatgc gggacaagaa aatctctttt
    21721 ctggtctgac ggcgcttact gctgaattca ctgtcggcga aggtaagttg atgactcatg
    21781 atgaaccctg ttctatggct ccagatgaca aacatgatct catatcaggg acttgttcgc
    21841 accttcctta gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa
    21901 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
    21961 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
    22021 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
    22081 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcaccttgaa
    22141 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
    22201 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
    22261 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
    22321 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
    22381 gaagacaccc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
    22441 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
    22501 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
    22561 attgccttct gcgtccggtc gatcagggag gatattgggg aagaacagta tgtcgagcta
    22621 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
    22681 gatgaattgt tttagtacct agatgtggcg caacgatgct ggcgacaagc aggagcgcac
    22741 cgacttcttc cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg
    22801 caaggggtcg ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg
    22861 ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc
    22921 accaggcgga tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc
    22981 gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga
    23041 cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc
    23101 ccgcgaaacc ttccagtccg tcggctcgat ggcccagcaa gctacggcca agatcgagcg
    23161 cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc
    23221 gcgtcgtctc gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg
    23281 aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga
    23341 ggccaagcaa gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc
    23401 cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg
    23461 ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt
    23521 cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga
    23581 cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga
    23641 gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg
    23701 gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac
    23761 gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga
    23821 ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt
    23881 tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc
    23941 ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac
    24001 cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg
    24061 cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga
    24121 cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc
    24181 cagcgcttta ctgagatcct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    24241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    24301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    24361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    24421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    24481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    24541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    24601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    24661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    24721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    24781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    24841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    24901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    24961 aggatctcaa
//
