LOCUS       sequence-426230-       10854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector expressing the Lineage Tracing system. No Cas9 control
            expression cassette. LT0 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10854)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 10854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: LT0
FEATURES             Location/Qualifiers
     source          1..10854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..86)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(240..828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(320..339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1042..1066
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1119..1141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_RNA        1815..1890
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(3453..3455,3456..3458,3459..4169)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3498..3519)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4106..4127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5354..6064
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5499..5517)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5767..5786
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    6308..6355
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    6467..6491
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6571..7202
                     /label=oriV
                     /note="incP origin of replication"
     CDS             8613..9407
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             9706..10854
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact gagatcctct
       61 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      901 atgagattat caaaaaggat cttcacctag atccttttgg atctcctgtg gttggcatgc
      961 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
     1021 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
     1081 aaactgaagg cgggaaacga caatctgcta gtggatctcc cagtcacgac gttgtaaaac
     1141 gggcgccccg cggaaagctt gctagccaat tggggcccaa cgttctcgag tttttctaga
     1201 aggccttgga tccacccggg agaattcgtc gactttgcgg ccgcatcgat actgcaggag
     1261 ctcggtacct tttactagtg atatcgatca tgagcgacct gtattcagtc gactggtacc
     1321 aacatcagct ttttttcttc ttcttcgttc atacagtttt tttttgttta tcagcttaca
     1381 ttttcttgaa ccgtagcttt cgttttcttc tttttaactt tccattcgga gtttttgtat
     1441 cttgtttcat agtttgtccc aggattagaa tgattaggca tcgaaccttc aagaatttga
     1501 ttgaataaaa catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat
     1561 ctgaaagaag agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg
     1621 cccatttaag ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc
     1681 tgagtttata tacagctaga gtcgaagtag tgattggaac aaagcaccag tggtctagtg
     1741 gtagaatagt accctgccac ggtacagacc cgggttcgat tcccggctgg tgcagtcccc
     1801 tccaccccac agtggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1861 caacttgaaa aagtggcacc gagtcggtgc tttttttttt tttctgcttt ctgcccttgc
     1921 caatccatat ctgaagaaga aaaaaaaaca caaaattacg atttagatcc gatataacaa
     1981 aatttgaatc gcacagatcg atctctttgg agattctata cctagaaaat ggagacgatt
     2041 ttcaaatctc tgtaaaaatt ctggtttctt cttgacggaa gaagacgacg actccaatat
     2101 ttcggttagt actgaaccgg aaagtttgac tggtgcaacc aatttaatgt accgtacgta
     2161 acgcaccaat cggattttgt attcaatggg ccttatctgt gagcccatta attgatgtga
     2221 cggcctaaac taaatccgaa cggtttattt cagcgatccg cgacggtttg tattcagcca
     2281 atagcaatca attatgtagc agtggtgatc ctcgtcaaac cagtaaagct agatctggac
     2341 cgttgaattg gtgcaagaaa gcacatgttg tgatattttt acccgtacga ttagaaaact
     2401 tgagaaacac attgataatc gataaaaacc gtccgatcat ataaatccgc tttaccatcg
     2461 ttgcctataa attaatatca atagccgtac acgcgtgaag actgacaata ttatcttttt
     2521 cgaattcgga gctcaagttt gaaattcgga gaagctagag agttttctga ggtacgattc
     2581 ttcgatcctc tttgattttc ctggaaatat tttttcggtg atcgtgaaac tactggaatc
     2641 gctcgatagg tggtacgaaa ttaggcgaga ttagtttcta ttcttggcca ttatcttgtt
     2701 tcttcgccga atgatcttcc ggataaagat tttaggttag agatgaatcg tatagctaga
     2761 tttcatcacc agatagtttc tttgtctaga atctctgaaa ttctcgatag ttttcacatg
     2821 tgtaaataga ttgttcttat tcggcgattg ttgattaggg ttttgatttt cttgattatg
     2881 cgattgcaat tagggatttt ctttggtttt gtgttgatct tacgatacat tccggcaatt
     2941 gaatacgtat ggatctaaat cttgttaatt tgttgaacag gacgatgcgt cccctccacc
     3001 ccacagtggg ggcaccaaga gccgagaaga agcccgccga gaaaaaaacc gccgctgaga
     3061 ggccggtgga agagaacaag gctgctgaaa aggctccggc ggagaagaaa cccaaggcgg
     3121 ggaagaaatt gccaccaaag gaagccggtg acaagaagaa gaagcgatct aagaaaaacg
     3181 tcgaaaccta caagatctac atcttcaagg tgctgaagca ggttcatcca gatatcggaa
     3241 tttcaagcaa agccatgggg attatgaaca gtttcatcaa cgacatattc gagaagctag
     3301 ctcaggaatc ttcgaaactc gctaggtaca acaagaagcc gaccattaca tctcgcgaga
     3361 tccagacggc tgtgagactc gtcctccccg gtgaactcgc taaacacgcc gtctctgaag
     3421 gtaccaaggc ggttaccaag tttactagct ccatggtgag caagggcgag gagctgttca
     3481 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3541 tccgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca
     3601 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgg
     3661 cctgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3721 ccgaaggcta cgtccaggag cgcaccatct ctttcaagga cgacggtacc tacaagaccc
     3781 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3841 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca
     3901 acgtctatat cacggccgac aagcagaaga acggcatcaa ggctaacttc aagatccgcc
     3961 acaacgttga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4021 gcgacggccc cgtgctgctg cccgacaacc actacctgag ccatcagtcc gccctgagca
     4081 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4141 ttacacatgg catggacgag ctgtacaagt aatcaggtgg atcctagata acctttacct
     4201 tcattccctg taaattggta ccctgcatct cgtctctgtt atgcttaaga agttcaatgt
     4261 ttcgtttcat gtaaaacttt ggtggtttgt gttttggggc cttgtataat ccctgatgaa
     4321 taagtgttct actatgtttc cgttcctgtt atctctttct ttctaatgac aagtcgaact
     4381 tcttctttat catcgcttcg tttttattat ctgtgcttct tttgtttaat acgcctgcaa
     4441 agtgactcga ctctgtttag tgcagttctg cgaaacttgt aaatagtcca attgttggcc
     4501 tctagtaata gatgtagcga aagtgttgag ctgttgggtt ctaaggatgg cttgaacatg
     4561 ttaatctttt aggttctgag tatgatgaac attcgttgtt gctaagaaat gcctgtaatg
     4621 tcccacaaat gtagaaaatg gttcgtacct ttgtccaagc attgatatgt ctgatgagag
     4681 gaaactgcaa gatactgagc ttggtttaac gaaggagagg cagtttcttc cttccaaagc
     4741 atttcatttg acaatgcctt gatcatctta agtagagttt ctgttgtgga aagtttgaaa
     4801 ctttgaagaa acgactctca agtaaattga tgatcacaag tgaaagtgta tgttactaac
     4861 tattgaaacc aaattaacat agggttttta tttaaataaa agtttaacct tctttttaaa
     4921 aaattgttca tagtgtcatg tcagaacaag agctacaaat cacacatagc atgcataagc
     4981 ggagctatga tgagtggtat tgttttgttc gtcacttgtc actcttttcc aacacataat
     5041 cccgacaaca acgtaagagc atctctctct ctccacacac actcatgcat gcatgcattc
     5101 ttacacgtga ttgccatgca aatctccttt ctcacctata aatacaaacc aacccttcac
     5161 tacactcttc actcaaacca aaacaagaaa acatacacaa atagcaaaac aagggcgaat
     5221 tctcaacaca acatatacaa aacaaacgaa tctcaagcaa tcaagcattc tacttctatt
     5281 gcagcaattt aaatcatttc ttttaaagca aaagcaattt tctgaaaatt ttcaccattt
     5341 acgaacgata gccatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     5401 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     5461 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     5521 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     5581 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     5641 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     5701 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     5761 cgacggcccc gtaatgcaga agaagacgat gggctgggag gcctcctccg agcggatgta
     5821 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     5881 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     5941 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     6001 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     6061 gtagcttggc tgcaggtcga cctgcaggca tgcccgctga aatcaccagt ctctctctac
     6121 aaatctatct ctctctataa taatgtgtga gtagttccca gataagggaa ttagggttct
     6181 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     6241 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccaggg gtaccgtatg
     6301 atctagggaa gttcctattc cgaagttcct attctctgaa aagtatagga acttctttgc
     6361 gtattgggcg ctcttggcct ttttggccac cggtcgtacg gttaaaacca ccccagtaca
     6421 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     6481 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     6541 tcgatacagg cagcccatca gtccactaga gacgctcacc gggctggttg ccctcgccgc
     6601 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc
     6661 gagacaccgc agccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag
     6721 atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga
     6781 ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa
     6841 cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct
     6901 gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact
     6961 tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac
     7021 aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt
     7081 cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct
     7141 gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg
     7201 ctctcgcgtt ggcagcatca cccataattg tggtttcaaa atcggctccg tcgatactat
     7261 gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt taaagtttta
     7321 gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagggaagg tgcgaataag
     7381 cggggaaatt cttctcggct gactcagtca tttcatttct tcatgtttga gccgattttt
     7441 tctcccgtaa atgccttgaa tcagcctatt tagaccgttt cttcgccatt taaggcgtta
     7501 tccccagttt ttagtgagat ctctcccact gacgtatcat ttggtccgcc cgaaacaggt
     7561 tggccagcgt gaataacatc gccagttggt tatcgttttt cagcaacccc ttgtatctgg
     7621 ctttcacgaa gccgaactgt cgcttgatga tgcgaaatgg gtgctccacc ctggcccgga
     7681 tgctggcttt catgtattcg atgttgatgg ccgttttgtt cttgcgtgga tgctgtttca
     7741 aggttcttac cttgccgggg cgctcggcga tcagccagtc cacatccacc tcggccagct
     7801 cctcgcgctg tggcgcccct tggtagccgg catcggctga gacaaattgc tcctctccat
     7861 gcagcagatt acccagctga ttgaggtcat gctcgttggc cgcggtggtg accaggctgt
     7921 gggtcaggcc actcttggca tcgacaccaa tgtgggcctt catgccaaag tgccactgat
     7981 tgcctttctt ggtctgatgc atctccggat cgcgttgctg ctctttgttc ttggtcgagc
     8041 tgggtgcctc aatgatggtg gcatcgacca aggtgccttg agtcatcatg acgcctgctt
     8101 cggccagcca gcgattgatg gtcttgaaca attggcgggc cagttgatgc tgctccagca
     8161 ggtggcggaa attcatgatg gtggtgcggt ccggcaaggc gctatccagg gataaccggg
     8221 caaacagacg catggaggcg atttcgtaca gagcatcttc catcgcgcca tcgctcaggt
     8281 tgtaccaatg ctgcatgcag tgaatgcgta gcatggtttc cagcggataa ggtcgccggc
     8341 cattaccagc cttggggtaa aacggctcga tgacttccac catgttttgc catggcagaa
     8401 tctgctccat gcgggacaag aaaatctctt ttctggtctg acggcgctta ctgctgaatt
     8461 cactgtcggc gaaggtaagt tgatgactca tgatgaaccc tgttctatgg ctccagatga
     8521 caaacatgat ctcatatcag ggacttgttc gcaccttcct tagcttcttg gggtatcttt
     8581 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
     8641 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
     8701 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
     8761 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
     8821 agctgcctgt tccaaaggtc ctgcaccttg aacggcatga tggctggagc aatctgctca
     8881 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
     8941 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
     9001 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
     9061 atctggccga tgtggattgc gaaaactggg aagaagacac cccatttaaa gatccgcgcg
     9121 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
     9181 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
     9241 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
     9301 aggatattgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     9361 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagatgtgg
     9421 cgcaacgatg ctggcgacaa gcaggagcgc accgacttct tccgcatcaa gtgttttggc
     9481 tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt cgtgcagggc
     9541 aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac cgacttcatt
     9601 gccgataagg tggattatct ggacaccaag gcaccaggcg gatcaaatca ggaataaggg
     9661 cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt
     9721 gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga ggatgccgaa
     9781 accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg
     9841 atggcccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc tccccctgcc
     9901 ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt
     9961 ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc
    10021 gccggcgagg acctggcaaa acaggtcagc gaggccaagc aagccgcgtt gctgaaacac
    10081 acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc gtggccggac
    10141 acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac gcgcaacaag
    10201 aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag
    10261 atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg
    10321 ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt ctacgagctt
    10381 tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga ggaatgcctg
    10441 tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca cctggaatcg
    10501 gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag
    10561 gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac gaaattcata
    10621 tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta tttcagctcg
    10681 caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg atcggattcc
    10741 acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc
    10801 ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg ctag
//
