LOCUS       sequence-426231-       10855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector expressing the Lineage Tracing system. No Cas9 control
            expression cassette. LT2 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 10855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: LT2
FEATURES             Location/Qualifiers
     source          1..10855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..86)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(240..828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(320..339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1042..1066
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1119..1141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_RNA        1815..1890
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(3454..3456,3457..3459,3460..4170)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3499..3520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4107..4128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5355..6065
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5500..5518)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5768..5787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    6309..6356
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    6468..6492
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6572..7203
                     /label=oriV
                     /note="incP origin of replication"
     CDS             8614..9408
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             9707..10855
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact gagatcctct
       61 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      901 atgagattat caaaaaggat cttcacctag atccttttgg atctcctgtg gttggcatgc
      961 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
     1021 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
     1081 aaactgaagg cgggaaacga caatctgcta gtggatctcc cagtcacgac gttgtaaaac
     1141 gggcgccccg cggaaagctt gctagccaat tggggcccaa cgttctcgag tttttctaga
     1201 aggccttgga tccacccggg agaattcgtc gactttgcgg ccgcatcgat actgcaggag
     1261 ctcggtacct tttactagtg atatcgatca tgagcgacct gtattcagtc gactggtacc
     1321 aacatcagct ttttttcttc ttcttcgttc atacagtttt tttttgttta tcagcttaca
     1381 ttttcttgaa ccgtagcttt cgttttcttc tttttaactt tccattcgga gtttttgtat
     1441 cttgtttcat agtttgtccc aggattagaa tgattaggca tcgaaccttc aagaatttga
     1501 ttgaataaaa catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat
     1561 ctgaaagaag agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg
     1621 cccatttaag ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc
     1681 tgagtttata tacagctaga gtcgaagtag tgattggaac aaagcaccag tggtctagtg
     1741 gtagaatagt accctgccac ggtacagacc cgggttcgat tcccggctgg tgcagtcccc
     1801 tccaccccac agtggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1861 caacttgaaa aagtggcacc gagtcggtgc tttttttttt tttctgcttt ctgcccttgc
     1921 caatccatat ctgaagaaga aaaaaaaaca caaaattacg atttagatcc gatataacaa
     1981 aatttgaatc gcacagatcg atctctttgg agattctata cctagaaaat ggagacgatt
     2041 ttcaaatctc tgtaaaaatt ctggtttctt cttgacggaa gaagacgacg actccaatat
     2101 ttcggttagt actgaaccgg aaagtttgac tggtgcaacc aatttaatgt accgtacgta
     2161 acgcaccaat cggattttgt attcaatggg ccttatctgt gagcccatta attgatgtga
     2221 cggcctaaac taaatccgaa cggtttattt cagcgatccg cgacggtttg tattcagcca
     2281 atagcaatca attatgtagc agtggtgatc ctcgtcaaac cagtaaagct agatctggac
     2341 cgttgaattg gtgcaagaaa gcacatgttg tgatattttt acccgtacga ttagaaaact
     2401 tgagaaacac attgataatc gataaaaacc gtccgatcat ataaatccgc tttaccatcg
     2461 ttgcctataa attaatatca atagccgtac acgcgtgaag actgacaata ttatcttttt
     2521 cgaattcgga gctcaagttt gaaattcgga gaagctagag agttttctga ggtacgattc
     2581 ttcgatcctc tttgattttc ctggaaatat tttttcggtg atcgtgaaac tactggaatc
     2641 gctcgatagg tggtacgaaa ttaggcgaga ttagtttcta ttcttggcca ttatcttgtt
     2701 tcttcgccga atgatcttcc ggataaagat tttaggttag agatgaatcg tatagctaga
     2761 tttcatcacc agatagtttc tttgtctaga atctctgaaa ttctcgatag ttttcacatg
     2821 tgtaaataga ttgttcttat tcggcgattg ttgattaggg ttttgatttt cttgattatg
     2881 cgattgcaat tagggatttt ctttggtttt gtgttgatct tacgatacat tccggcaatt
     2941 gaatacgtat ggatctaaat cttgttaatt tgttgaacag gacgatgggg tcccctccac
     3001 cccacagtgg gggcaccaag agccgagaag aagcccgccg agaaaaaaac cgccgctgag
     3061 aggccggtgg aagagaacaa ggctgctgaa aaggctccgg cggagaagaa acccaaggcg
     3121 gggaagaaat tgccaccaaa ggaagccggt gacaagaaga agaagcgatc taagaaaaac
     3181 gtcgaaacct acaagatcta catcttcaag gtgctgaagc aggttcatcc agatatcgga
     3241 atttcaagca aagccatggg gattatgaac agtttcatca acgacatatt cgagaagcta
     3301 gctcaggaat cttcgaaact cgctaggtac aacaagaagc cgaccattac atctcgcgag
     3361 atccagacgg ctgtgagact cgtcctcccc ggtgaactcg ctaaacacgc cgtctctgaa
     3421 ggtaccaagg cggttaccaa gtttactagc tccatggtga gcaagggcga ggagctgttc
     3481 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     3541 gtccgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
     3601 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg
     3661 gcctgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3721 cccgaaggct acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc
     3781 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3841 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
     3901 aacgtctata tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc
     3961 cacaacgttg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     4021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc cgccctgagc
     4081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4141 attacacatg gcatggacga gctgtacaag taatcaggtg gatcctagat aacctttacc
     4201 ttcattccct gtaaattggt accctgcatc tcgtctctgt tatgcttaag aagttcaatg
     4261 tttcgtttca tgtaaaactt tggtggtttg tgttttgggg ccttgtataa tccctgatga
     4321 ataagtgttc tactatgttt ccgttcctgt tatctctttc tttctaatga caagtcgaac
     4381 ttcttcttta tcatcgcttc gtttttatta tctgtgcttc ttttgtttaa tacgcctgca
     4441 aagtgactcg actctgttta gtgcagttct gcgaaacttg taaatagtcc aattgttggc
     4501 ctctagtaat agatgtagcg aaagtgttga gctgttgggt tctaaggatg gcttgaacat
     4561 gttaatcttt taggttctga gtatgatgaa cattcgttgt tgctaagaaa tgcctgtaat
     4621 gtcccacaaa tgtagaaaat ggttcgtacc tttgtccaag cattgatatg tctgatgaga
     4681 ggaaactgca agatactgag cttggtttaa cgaaggagag gcagtttctt ccttccaaag
     4741 catttcattt gacaatgcct tgatcatctt aagtagagtt tctgttgtgg aaagtttgaa
     4801 actttgaaga aacgactctc aagtaaattg atgatcacaa gtgaaagtgt atgttactaa
     4861 ctattgaaac caaattaaca tagggttttt atttaaataa aagtttaacc ttctttttaa
     4921 aaaattgttc atagtgtcat gtcagaacaa gagctacaaa tcacacatag catgcataag
     4981 cggagctatg atgagtggta ttgttttgtt cgtcacttgt cactcttttc caacacataa
     5041 tcccgacaac aacgtaagag catctctctc tctccacaca cactcatgca tgcatgcatt
     5101 cttacacgtg attgccatgc aaatctcctt tctcacctat aaatacaaac caacccttca
     5161 ctacactctt cactcaaacc aaaacaagaa aacatacaca aatagcaaaa caagggcgaa
     5221 ttctcaacac aacatataca aaacaaacga atctcaagca atcaagcatt ctacttctat
     5281 tgcagcaatt taaatcattt cttttaaagc aaaagcaatt ttctgaaaat tttcaccatt
     5341 tacgaacgat agccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     5401 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     5461 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     5521 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     5581 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     5641 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     5701 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     5761 ccgacggccc cgtaatgcag aagaagacga tgggctggga ggcctcctcc gagcggatgt
     5821 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     5881 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     5941 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     6001 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     6061 agtagcttgg ctgcaggtcg acctgcaggc atgcccgctg aaatcaccag tctctctcta
     6121 caaatctatc tctctctata ataatgtgtg agtagttccc agataaggga attagggttc
     6181 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     6241 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagg ggtaccgtat
     6301 gatctaggga agttcctatt ccgaagttcc tattctctga aaagtatagg aacttctttg
     6361 cgtattgggc gctcttggcc tttttggcca ccggtcgtac ggttaaaacc accccagtac
     6421 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     6481 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     6541 ctcgatacag gcagcccatc agtccactag agacgctcac cgggctggtt gccctcgccg
     6601 ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg
     6661 cgagacaccg cagccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
     6721 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
     6781 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa
     6841 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
     6901 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
     6961 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
     7021 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
     7081 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
     7141 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
     7201 gctctcgcgt tggcagcatc acccataatt gtggtttcaa aatcggctcc gtcgatacta
     7261 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaagtttt
     7321 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagggaag gtgcgaataa
     7381 gcggggaaat tcttctcggc tgactcagtc atttcatttc ttcatgtttg agccgatttt
     7441 ttctcccgta aatgccttga atcagcctat ttagaccgtt tcttcgccat ttaaggcgtt
     7501 atccccagtt tttagtgaga tctctcccac tgacgtatca tttggtccgc ccgaaacagg
     7561 ttggccagcg tgaataacat cgccagttgg ttatcgtttt tcagcaaccc cttgtatctg
     7621 gctttcacga agccgaactg tcgcttgatg atgcgaaatg ggtgctccac cctggcccgg
     7681 atgctggctt tcatgtattc gatgttgatg gccgttttgt tcttgcgtgg atgctgtttc
     7741 aaggttctta ccttgccggg gcgctcggcg atcagccagt ccacatccac ctcggccagc
     7801 tcctcgcgct gtggcgcccc ttggtagccg gcatcggctg agacaaattg ctcctctcca
     7861 tgcagcagat tacccagctg attgaggtca tgctcgttgg ccgcggtggt gaccaggctg
     7921 tgggtcaggc cactcttggc atcgacacca atgtgggcct tcatgccaaa gtgccactga
     7981 ttgcctttct tggtctgatg catctccgga tcgcgttgct gctctttgtt cttggtcgag
     8041 ctgggtgcct caatgatggt ggcatcgacc aaggtgcctt gagtcatcat gacgcctgct
     8101 tcggccagcc agcgattgat ggtcttgaac aattggcggg ccagttgatg ctgctccagc
     8161 aggtggcgga aattcatgat ggtggtgcgg tccggcaagg cgctatccag ggataaccgg
     8221 gcaaacagac gcatggaggc gatttcgtac agagcatctt ccatcgcgcc atcgctcagg
     8281 ttgtaccaat gctgcatgca gtgaatgcgt agcatggttt ccagcggata aggtcgccgg
     8341 ccattaccag ccttggggta aaacggctcg atgacttcca ccatgttttg ccatggcaga
     8401 atctgctcca tgcgggacaa gaaaatctct tttctggtct gacggcgctt actgctgaat
     8461 tcactgtcgg cgaaggtaag ttgatgactc atgatgaacc ctgttctatg gctccagatg
     8521 acaaacatga tctcatatca gggacttgtt cgcaccttcc ttagcttctt ggggtatctt
     8581 taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa
     8641 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
     8701 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
     8761 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
     8821 aagctgcctg ttccaaaggt cctgcacctt gaacggcatg atggctggag caatctgctc
     8881 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
     8941 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
     9001 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
     9061 gatctggccg atgtggattg cgaaaactgg gaagaagaca ccccatttaa agatccgcgc
     9121 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
     9181 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
     9241 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg
     9301 gaggatattg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     9361 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta cctagatgtg
     9421 gcgcaacgat gctggcgaca agcaggagcg caccgacttc ttccgcatca agtgttttgg
     9481 ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg
     9541 caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga ccgacttcat
     9601 tgccgataag gtggattatc tggacaccaa ggcaccaggc ggatcaaatc aggaataagg
     9661 gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga atcggacgtt
     9721 tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg aggatgccga
     9781 aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc
     9841 gatggcccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc
     9901 cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg
     9961 tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga agcgaaaaac
    10021 cgccggcgag gacctggcaa aacaggtcag cgaggccaag caagccgcgt tgctgaaaca
    10081 cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc cgtggccgga
    10141 cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa
    10201 gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa
    10261 gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt ggcagcaggt
    10321 gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt tctacgagct
    10381 ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg aggaatgcct
    10441 gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc acctggaatc
    10501 ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca
    10561 ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca cgaaattcat
    10621 atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact atttcagctc
    10681 gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg gatcggattc
    10741 cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag
    10801 cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac gctag
//
