LOCUS       sequence-426233-       16442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector expressing the Lineage Tracing system. Cas9 expression
            driven by CLV3 promoter. LT1 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16442)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 16442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: pCLV3Cas-LT2
FEATURES             Location/Qualifiers
     source          1..16442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..86)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(240..828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(320..339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1042..1066
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1119..1141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2277..6380
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2277..6380
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6393..6413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        7402..7477
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(9041..9043,9044..9046,9047..9757)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(9086..9107)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     9694..9715
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10942..11652
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11087..11105)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11355..11374
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    11896..11943
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    12055..12079
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      12159..12790
                     /label=oriV
                     /note="incP origin of replication"
     CDS             14201..14995
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             15294..16442
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact gagatcctct
       61 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      901 atgagattat caaaaaggat cttcacctag atccttttgg atctcctgtg gttggcatgc
      961 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
     1021 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
     1081 aaactgaagg cgggaaacga caatctgcta gtggatctcc cagtcacgac gttgtaaaac
     1141 gggcgccccg cggaaagctt gctagccaat tggggcccaa cgttctcgag tttttctaga
     1201 aggccttgga tccacccggg agaattcgtc gactttgcgg ccgcatcgat actgcaggag
     1261 ctcggtacct tttactagtg atatcgatca tgagcgacct aaaaattacg gatatgaata
     1321 taggcatatc cgtatccgaa ttatccgttt gacagctagc aacgattgta caattgcttc
     1381 tttaaaaaag gaagaaagaa agaaagaaaa gaatcaacat cagcgttaac aaacggcccc
     1441 gttacggccc aaacggtcat atagagtaac ggcgttaagc gttgaaagac tcctatcgaa
     1501 atacgtaacc gcaaacgtgt catagtcaga tcccctcttc cttcaccgcc tcaaacacaa
     1561 aaataatctt ctacagccta tatatacaac ccccccttct atctctcctt tctcacaatt
     1621 catcatcttt ctttctctac ccccaatttt aagaaatcct ctcttctcct cttcattttc
     1681 aaggtaaatc tctctctctc tctctctctc tgttattcct tgttttaatt aggtatgtat
     1741 tattgctagt ttgttaatct gcttatctta tgtatgcctt atgtgaatat ctttatcttg
     1801 ttcatctcat ccgtttagaa gctataaatt tgttgatttg actgtgtatc tacacgtggt
     1861 tatgtttata tctaatcaga tatgaatttc ttcatattgt tgcgtttgtg tgtaccaatc
     1921 cgaaatcgtt gatttttttc atttaatcgt gtagctaatt gtacgtatac atatggatct
     1981 acgtatcaat tgttcatctg tttgtgtttg tatgtataca gatctgaaaa catcacttct
     2041 ctcatctgat tgtgttgtta catacataga tatagatctg ttatatcatt ttttttatta
     2101 attgtgtata tatatatgtg catagatctg gattacatga ttgtgattat ttacatgatt
     2161 ttgttattta cgtatgtata tatgtagatc tggacttttt ggagttgttg acttgattgt
     2221 atttgtgtgt gtatatgtgt gttctgatct tgatatgtta tgtatgtgca gcaacaatgg
     2281 ataagaagta ctctatcgga ctcgatatcg gaactaactc tgtgggatgg gctgtgatca
     2341 ccgatgagta caaggtgcca tctaagaagt tcaaggttct cggaaacacc gataggcact
     2401 ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc tggtgaaact gctgaggcta
     2461 ccagactcaa gagaaccgct agaagaaggt acaccagaag aaagaacagg atctgctacc
     2521 tccaagagat cttctctaac gagatggcta aagtggatga ttcattcttc cacaggctcg
     2581 aagagtcatt cctcgtggaa gaagataaga agcacgagag gcaccctatc ttcggaaaca
     2641 tcgttgatga ggtggcatac cacgagaagt accctactat ctaccacctc agaaagaagc
     2701 tcgttgattc tactgataag gctgatctca ggctcatcta cctcgctctc gctcacatga
     2761 tcaagttcag aggacacttc ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg
     2821 ataagttgtt catccagctc gtgcagacct acaaccagct tttcgaagag aaccctatca
     2881 acgcttcagg tgtggatgct aaggctatcc tctctgctag gctctctaag tcaagaaggc
     2941 ttgagaacct cattgctcag ctccctggtg agaagaagaa cggacttttc ggaaacttga
     3001 tcgctctctc tctcggactc acccctaact tcaagtctaa cttcgatctc gctgaggatg
     3061 caaagctcca gctctcaaag gatacctacg atgatgatct cgataacctc ctcgctcaga
     3121 tcggagatca gtacgctgat ttgttcctcg ctgctaagaa cctctctgat gctatcctcc
     3181 tcagtgatat cctcagagtg aacaccgaga tcaccaaggc tccactctca gcttctatga
     3241 tcaagagata cgatgagcac caccaggatc tcacacttct caaggctctt gttagacagc
     3301 agctcccaga gaagtacaaa gagattttct tcgatcagtc taagaacgga tacgctggtt
     3361 acatcgatgg tggtgcatct caagaagagt tctacaagtt catcaagcct atcctcgaga
     3421 agatggatgg aaccgaggaa ctcctcgtga agctcaatag agaggatctt ctcagaaagc
     3481 agaggacctt cgataacgga tctatccctc atcagatcca cctcggagag ttgcacgcta
     3541 tccttagaag gcaagaggat ttctacccat tcctcaagga taacagggaa aagattgaga
     3601 agattctcac cttcagaatc ccttactacg tgggacctct cgctagagga aactcaagat
     3661 tcgcttggat gaccagaaag tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg
     3721 tggataaggg tgctagtgct cagtctttca tcgagaggat gaccaacttc gataagaacc
     3781 ttccaaacga gaaggtgctc cctaagcact ctttgctcta cgagtacttc accgtgtaca
     3841 acgagttgac caaggttaag tacgtgaccg agggaatgag gaagcctgct tttttgtcag
     3901 gtgagcaaaa gaaggctatc gttgatctct tgttcaagac caacagaaag gtgaccgtga
     3961 agcagctcaa agaggattac ttcaagaaaa tcgagtgctt cgattcagtt gagatttctg
     4021 gtgttgagga taggttcaac gcatctctcg gaacctacca cgatctcctc aagatcatta
     4081 aggataagga tttcttggat aacgaggaaa acgaggatat cttggaggat atcgttctta
     4141 ccctcaccct ctttgaagat agagagatga ttgaagaaag gctcaagacc tacgctcatc
     4201 tcttcgatga taaggtgatg aagcagttga agagaagaag atacactggt tggggaaggc
     4261 tctcaagaaa gctcattaac ggaatcaggg ataagcagtc tggaaagaca atccttgatt
     4321 tcctcaagtc tgatggattc gctaacagaa acttcatgca gctcatccac gatgattctc
     4381 tcacctttaa agaggatatc cagaaggctc aggtttcagg acagggtgat agtctccatg
     4441 agcatatcgc taacctcgct ggatctcctg caatcaagaa gggaatcctc cagactgtga
     4501 aggttgtgga tgagttggtg aaggtgatgg gaaggcataa gcctgagaac atcgtgatcg
     4561 aaatggctag agagaaccag accactcaga agggacagaa gaactctagg gaaaggatga
     4621 agaggatcga ggaaggtatc aaagagcttg gatctcagat cctcaaagag caccctgttg
     4681 agaacactca gctccagaat gagaagctct acctctacta cctccagaac ggaagggata
     4741 tgtatgtgga tcaagagttg gatatcaaca ggctctctga ttacgatgtt gatcatatcg
     4801 tgccacagtc attcttgaag gatgattcta tcgataacaa ggtgctcacc aggtctgata
     4861 agaacagggg taagagtgat aacgtgccaa gtgaagaggt tgtgaagaaa atgaagaact
     4921 attggaggca gctcctcaac gctaagctca tcactcagag aaagttcgat aacttgacta
     4981 aggctgagag gggaggactc tctgaattgg ataaggcagg attcatcaag aggcagcttg
     5041 tggaaaccag gcagatcact aagcacgttg cacagatcct cgattctagg atgaacacca
     5101 agtacgatga gaacgataag ttgatcaggg aagtgaaggt tatcaccctc aagtcaaagc
     5161 tcgtgtctga tttcagaaag gatttccaat tctacaaggt gagggaaatc aacaactacc
     5221 accacgctca cgatgcttac cttaacgctg ttgttggaac cgctctcatc aagaagtatc
     5281 ctaagctcga gtcagagttc gtgtacggtg attacaaggt gtacgatgtg aggaagatga
     5341 tcgctaagtc tgagcaagag atcggaaagg ctaccgctaa gtatttcttc tactctaaca
     5401 tcatgaattt cttcaagacc gagattaccc tcgctaacgg tgagatcaga aagaggccac
     5461 tcatcgagac aaacggtgaa acaggtgaga tcgtgtggga taagggaagg gatttcgcta
     5521 ccgttagaaa ggtgctctct atgccacagg tgaacatcgt taagaaaacc gaggtgcaga
     5581 ccggtggatt ctctaaagag tctatcctcc ctaagaggaa ctctgataag ctcattgcta
     5641 ggaagaagga ttgggaccct aagaaatacg gtggtttcga ttctcctacc gtggcttact
     5701 ctgttctcgt tgtggctaag gttgagaagg gaaagagtaa gaagctcaag tctgttaagg
     5761 aacttctcgg aatcactatc atggaaaggt catctttcga gaagaaccca atcgatttcc
     5821 tcgaggctaa gggatacaaa gaggttaaga aggatctcat catcaagctc ccaaagtact
     5881 cactcttcga actcgagaac ggtagaaaga ggatgctcgc ttctgctggt gagcttcaaa
     5941 agggaaacga gcttgctctc ccatctaagt acgttaactt tctttacctc gcttctcact
     6001 acgagaagtt gaagggatct ccagaagata acgagcagaa gcaacttttc gttgagcagc
     6061 acaagcacta cttggatgag atcatcgagc agatctctga gttctctaaa agggtgatcc
     6121 tcgctgatgc aaacctcgat aaggtgttgt ctgcttacaa caagcacaga gataagccta
     6181 tcagggaaca ggcagagaac atcatccatc tcttcaccct taccaacctc ggtgctcctg
     6241 ctgctttcaa gtacttcgat acaaccatcg ataggaagag atacacctct accaaagaag
     6301 tgctcgatgc taccctcatc catcagtcta tcactggact ctacgagact aggatcgatc
     6361 tctcacagct cggtggtgat tcaagggctg atcctaagaa gaagaggaag gtttgagggg
     6421 ctcacgacga actctaagag ctcaggcctc ccagctttcg tccgtatcat cggtttcgac
     6481 aacgttcgtc aagttcaatg catcagtttc attgcccaca caccagaatc ctactaagtt
     6541 tgagtattat ggcattggaa aagctgtttt cttctatcat ttgttctgct tgtaatttac
     6601 tgtgttcttt cagtttttgt tttcggacat caaaatgcaa atggatggat aagagttaat
     6661 aaatgatatg gtccttttgt tcattctcaa attattatta tctgttgttt ttactttaat
     6721 gggttgaatt taagtaagaa aggaactaac agtgtgatat taaggtgcaa tgttagacat
     6781 ataaaacagt ctttcacctc tctttggtta tgtcttgaat tggtttgttt cttcacttat
     6841 ctgtgtaatc aagtttacta tgagtctatg atcaagtaat tatgcaatca agttaagtac
     6901 agtataggct ttcagctttt tttcttcttc ttcgttcata cagttttttt ttgtttatca
     6961 gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc attcggagtt
     7021 tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg aaccttcaag
     7081 aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc aaaaggcccc
     7141 tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat agtatttctt
     7201 atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt agataagaaa
     7261 acgaagctga gtttatatac agctagagtc gaagtagtga ttggaacaaa gcaccagtgg
     7321 tctagtggta gaatagtacc ctgccacggt acagacccgg gttcgattcc cggctggtgc
     7381 agtcccctcc accccacagt ggttttagag ctagaaatag caagttaaaa taaggctagt
     7441 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttttttttt ctgctttctg
     7501 cccttgccaa tccatatctg aagaagaaaa aaaaacacaa aattacgatt tagatccgat
     7561 ataacaaaat ttgaatcgca cagatcgatc tctttggaga ttctatacct agaaaatgga
     7621 gacgattttc aaatctctgt aaaaattctg gtttcttctt gacggaagaa gacgacgact
     7681 ccaatatttc ggttagtact gaaccggaaa gtttgactgg tgcaaccaat ttaatgtacc
     7741 gtacgtaacg caccaatcgg attttgtatt caatgggcct tatctgtgag cccattaatt
     7801 gatgtgacgg cctaaactaa atccgaacgg tttatttcag cgatccgcga cggtttgtat
     7861 tcagccaata gcaatcaatt atgtagcagt ggtgatcctc gtcaaaccag taaagctaga
     7921 tctggaccgt tgaattggtg caagaaagca catgttgtga tatttttacc cgtacgatta
     7981 gaaaacttga gaaacacatt gataatcgat aaaaaccgtc cgatcatata aatccgcttt
     8041 accatcgttg cctataaatt aatatcaata gccgtacacg cgtgaagact gacaatatta
     8101 tctttttcga attcggagct caagtttgaa attcggagaa gctagagagt tttctgaggt
     8161 acgattcttc gatcctcttt gattttcctg gaaatatttt ttcggtgatc gtgaaactac
     8221 tggaatcgct cgataggtgg tacgaaatta ggcgagatta gtttctattc ttggccatta
     8281 tcttgtttct tcgccgaatg atcttccgga taaagatttt aggttagaga tgaatcgtat
     8341 agctagattt catcaccaga tagtttcttt gtctagaatc tctgaaattc tcgatagttt
     8401 tcacatgtgt aaatagattg ttcttattcg gcgattgttg attagggttt tgattttctt
     8461 gattatgcga ttgcaattag ggattttctt tggttttgtg ttgatcttac gatacattcc
     8521 ggcaattgaa tacgtatgga tctaaatctt gttaatttgt tgaacaggac gatggggtcc
     8581 cctccacccc acagtggggg caccaagagc cgagaagaag cccgccgaga aaaaaaccgc
     8641 cgctgagagg ccggtggaag agaacaaggc tgctgaaaag gctccggcgg agaagaaacc
     8701 caaggcgggg aagaaattgc caccaaagga agccggtgac aagaagaaga agcgatctaa
     8761 gaaaaacgtc gaaacctaca agatctacat cttcaaggtg ctgaagcagg ttcatccaga
     8821 tatcggaatt tcaagcaaag ccatggggat tatgaacagt ttcatcaacg acatattcga
     8881 gaagctagct caggaatctt cgaaactcgc taggtacaac aagaagccga ccattacatc
     8941 tcgcgagatc cagacggctg tgagactcgt cctccccggt gaactcgcta aacacgccgt
     9001 ctctgaaggt accaaggcgg ttaccaagtt tactagctcc atggtgagca agggcgagga
     9061 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     9121 gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
     9181 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     9241 cggcgtggcc tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     9301 cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta
     9361 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     9421 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa
     9481 cagccacaac gtctatatca cggccgacaa gcagaagaac ggcatcaagg ctaacttcaa
     9541 gatccgccac aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     9601 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccgc
     9661 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     9721 cgccgggatt acacatggca tggacgagct gtacaagtaa tcaggtggat cctagataac
     9781 ctttaccttc attccctgta aattggtacc ctgcatctcg tctctgttat gcttaagaag
     9841 ttcaatgttt cgtttcatgt aaaactttgg tggtttgtgt tttggggcct tgtataatcc
     9901 ctgatgaata agtgttctac tatgtttccg ttcctgttat ctctttcttt ctaatgacaa
     9961 gtcgaacttc ttctttatca tcgcttcgtt tttattatct gtgcttcttt tgtttaatac
    10021 gcctgcaaag tgactcgact ctgtttagtg cagttctgcg aaacttgtaa atagtccaat
    10081 tgttggcctc tagtaataga tgtagcgaaa gtgttgagct gttgggttct aaggatggct
    10141 tgaacatgtt aatcttttag gttctgagta tgatgaacat tcgttgttgc taagaaatgc
    10201 ctgtaatgtc ccacaaatgt agaaaatggt tcgtaccttt gtccaagcat tgatatgtct
    10261 gatgagagga aactgcaaga tactgagctt ggtttaacga aggagaggca gtttcttcct
    10321 tccaaagcat ttcatttgac aatgccttga tcatcttaag tagagtttct gttgtggaaa
    10381 gtttgaaact ttgaagaaac gactctcaag taaattgatg atcacaagtg aaagtgtatg
    10441 ttactaacta ttgaaaccaa attaacatag ggtttttatt taaataaaag tttaaccttc
    10501 tttttaaaaa attgttcata gtgtcatgtc agaacaagag ctacaaatca cacatagcat
    10561 gcataagcgg agctatgatg agtggtattg ttttgttcgt cacttgtcac tcttttccaa
    10621 cacataatcc cgacaacaac gtaagagcat ctctctctct ccacacacac tcatgcatgc
    10681 atgcattctt acacgtgatt gccatgcaaa tctcctttct cacctataaa tacaaaccaa
    10741 cccttcacta cactcttcac tcaaaccaaa acaagaaaac atacacaaat agcaaaacaa
    10801 gggcgaattc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc aagcattcta
    10861 cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc tgaaaatttt
    10921 caccatttac gaacgatagc catggtgagc aagggcgagg aggataacat ggccatcatc
    10981 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
    11041 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
    11101 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
    11161 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
    11221 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
    11281 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
    11341 ttcccctccg acggccccgt aatgcagaag aagacgatgg gctgggaggc ctcctccgag
    11401 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
    11461 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
    11521 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
    11581 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
    11641 ctgtacaagt agcttggctg caggtcgacc tgcaggcatg cccgctgaaa tcaccagtct
    11701 ctctctacaa atctatctct ctctataata atgtgtgagt agttcccaga taagggaatt
    11761 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
    11821 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccaggggt
    11881 accgtatgat ctagggaagt tcctattccg aagttcctat tctctgaaaa gtataggaac
    11941 ttctttgcgt attgggcgct cttggccttt ttggccaccg gtcgtacggt taaaaccacc
    12001 ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
    12061 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
    12121 atcaccactc gatacaggca gcccatcagt ccactagaga cgctcaccgg gctggttgcc
    12181 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
    12241 ccgtgtgcga gacaccgcag ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
    12301 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
    12361 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
    12421 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
    12481 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
    12541 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
    12601 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
    12661 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
    12721 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
    12781 ccggcccgct ctcgcgttgg cagcatcacc cataattgtg gtttcaaaat cggctccgtc
    12841 gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta
    12901 aagttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt agggaaggtg
    12961 cgaataagcg gggaaattct tctcggctga ctcagtcatt tcatttcttc atgtttgagc
    13021 cgattttttc tcccgtaaat gccttgaatc agcctattta gaccgtttct tcgccattta
    13081 aggcgttatc cccagttttt agtgagatct ctcccactga cgtatcattt ggtccgcccg
    13141 aaacaggttg gccagcgtga ataacatcgc cagttggtta tcgtttttca gcaacccctt
    13201 gtatctggct ttcacgaagc cgaactgtcg cttgatgatg cgaaatgggt gctccaccct
    13261 ggcccggatg ctggctttca tgtattcgat gttgatggcc gttttgttct tgcgtggatg
    13321 ctgtttcaag gttcttacct tgccggggcg ctcggcgatc agccagtcca catccacctc
    13381 ggccagctcc tcgcgctgtg gcgccccttg gtagccggca tcggctgaga caaattgctc
    13441 ctctccatgc agcagattac ccagctgatt gaggtcatgc tcgttggccg cggtggtgac
    13501 caggctgtgg gtcaggccac tcttggcatc gacaccaatg tgggccttca tgccaaagtg
    13561 ccactgattg cctttcttgg tctgatgcat ctccggatcg cgttgctgct ctttgttctt
    13621 ggtcgagctg ggtgcctcaa tgatggtggc atcgaccaag gtgccttgag tcatcatgac
    13681 gcctgcttcg gccagccagc gattgatggt cttgaacaat tggcgggcca gttgatgctg
    13741 ctccagcagg tggcggaaat tcatgatggt ggtgcggtcc ggcaaggcgc tatccaggga
    13801 taaccgggca aacagacgca tggaggcgat ttcgtacaga gcatcttcca tcgcgccatc
    13861 gctcaggttg taccaatgct gcatgcagtg aatgcgtagc atggtttcca gcggataagg
    13921 tcgccggcca ttaccagcct tggggtaaaa cggctcgatg acttccacca tgttttgcca
    13981 tggcagaatc tgctccatgc gggacaagaa aatctctttt ctggtctgac ggcgcttact
    14041 gctgaattca ctgtcggcga aggtaagttg atgactcatg atgaaccctg ttctatggct
    14101 ccagatgaca aacatgatct catatcaggg acttgttcgc accttcctta gcttcttggg
    14161 gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc
    14221 accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc
    14281 tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga
    14341 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
    14401 ggaaggaaag ctgcctgttc caaaggtcct gcaccttgaa cggcatgatg gctggagcaa
    14461 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag
    14521 ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat
    14581 atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg attacttact
    14641 gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacaccc catttaaaga
    14701 tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc
    14761 ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat
    14821 tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc
    14881 gatcagggag gatattgggg aagaacagta tgtcgagcta ttttttgact tactggggat
    14941 caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct
    15001 agatgtggcg caacgatgct ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt
    15061 gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg ctggtattcg
    15121 tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc tacgggaccg
    15181 acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcgga tcaaatcagg
    15241 aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc
    15301 ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg
    15361 atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg
    15421 tcggctcgat ggcccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc
    15481 cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc gaacaggagg
    15541 cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc
    15601 gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcaa gccgcgttgc
    15661 tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt
    15721 ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc
    15781 gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg
    15841 acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc
    15901 agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct
    15961 acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg
    16021 aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc
    16081 tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc
    16141 gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga
    16201 aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt
    16261 tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat
    16321 cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc
    16381 gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct
    16441 ag
//
