LOCUS       sequence-426236-       16440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector expressing the Lineage Tracing system. Cas9 expression
            driven by CLV3 promoter. LT1 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16440)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 16440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: pCLV3Cas-LT1
FEATURES             Location/Qualifiers
     source          1..16440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..86)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(240..828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(320..339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1042..1066
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1119..1141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2277..6380
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2277..6380
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6393..6413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        7402..7477
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(9039..9041,9042..9044,9045..9755)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(9084..9105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     9692..9713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10940..11650
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11085..11103)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11353..11372
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    11894..11941
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    12053..12077
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      12157..12788
                     /label=oriV
                     /note="incP origin of replication"
     CDS             14199..14993
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             15292..16440
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact gagatcctct
       61 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      901 atgagattat caaaaaggat cttcacctag atccttttgg atctcctgtg gttggcatgc
      961 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
     1021 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
     1081 aaactgaagg cgggaaacga caatctgcta gtggatctcc cagtcacgac gttgtaaaac
     1141 gggcgccccg cggaaagctt gctagccaat tggggcccaa cgttctcgag tttttctaga
     1201 aggccttgga tccacccggg agaattcgtc gactttgcgg ccgcatcgat actgcaggag
     1261 ctcggtacct tttactagtg atatcgatca tgagcgacct aaaaattacg gatatgaata
     1321 taggcatatc cgtatccgaa ttatccgttt gacagctagc aacgattgta caattgcttc
     1381 tttaaaaaag gaagaaagaa agaaagaaaa gaatcaacat cagcgttaac aaacggcccc
     1441 gttacggccc aaacggtcat atagagtaac ggcgttaagc gttgaaagac tcctatcgaa
     1501 atacgtaacc gcaaacgtgt catagtcaga tcccctcttc cttcaccgcc tcaaacacaa
     1561 aaataatctt ctacagccta tatatacaac ccccccttct atctctcctt tctcacaatt
     1621 catcatcttt ctttctctac ccccaatttt aagaaatcct ctcttctcct cttcattttc
     1681 aaggtaaatc tctctctctc tctctctctc tgttattcct tgttttaatt aggtatgtat
     1741 tattgctagt ttgttaatct gcttatctta tgtatgcctt atgtgaatat ctttatcttg
     1801 ttcatctcat ccgtttagaa gctataaatt tgttgatttg actgtgtatc tacacgtggt
     1861 tatgtttata tctaatcaga tatgaatttc ttcatattgt tgcgtttgtg tgtaccaatc
     1921 cgaaatcgtt gatttttttc atttaatcgt gtagctaatt gtacgtatac atatggatct
     1981 acgtatcaat tgttcatctg tttgtgtttg tatgtataca gatctgaaaa catcacttct
     2041 ctcatctgat tgtgttgtta catacataga tatagatctg ttatatcatt ttttttatta
     2101 attgtgtata tatatatgtg catagatctg gattacatga ttgtgattat ttacatgatt
     2161 ttgttattta cgtatgtata tatgtagatc tggacttttt ggagttgttg acttgattgt
     2221 atttgtgtgt gtatatgtgt gttctgatct tgatatgtta tgtatgtgca gcaacaatgg
     2281 ataagaagta ctctatcgga ctcgatatcg gaactaactc tgtgggatgg gctgtgatca
     2341 ccgatgagta caaggtgcca tctaagaagt tcaaggttct cggaaacacc gataggcact
     2401 ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc tggtgaaact gctgaggcta
     2461 ccagactcaa gagaaccgct agaagaaggt acaccagaag aaagaacagg atctgctacc
     2521 tccaagagat cttctctaac gagatggcta aagtggatga ttcattcttc cacaggctcg
     2581 aagagtcatt cctcgtggaa gaagataaga agcacgagag gcaccctatc ttcggaaaca
     2641 tcgttgatga ggtggcatac cacgagaagt accctactat ctaccacctc agaaagaagc
     2701 tcgttgattc tactgataag gctgatctca ggctcatcta cctcgctctc gctcacatga
     2761 tcaagttcag aggacacttc ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg
     2821 ataagttgtt catccagctc gtgcagacct acaaccagct tttcgaagag aaccctatca
     2881 acgcttcagg tgtggatgct aaggctatcc tctctgctag gctctctaag tcaagaaggc
     2941 ttgagaacct cattgctcag ctccctggtg agaagaagaa cggacttttc ggaaacttga
     3001 tcgctctctc tctcggactc acccctaact tcaagtctaa cttcgatctc gctgaggatg
     3061 caaagctcca gctctcaaag gatacctacg atgatgatct cgataacctc ctcgctcaga
     3121 tcggagatca gtacgctgat ttgttcctcg ctgctaagaa cctctctgat gctatcctcc
     3181 tcagtgatat cctcagagtg aacaccgaga tcaccaaggc tccactctca gcttctatga
     3241 tcaagagata cgatgagcac caccaggatc tcacacttct caaggctctt gttagacagc
     3301 agctcccaga gaagtacaaa gagattttct tcgatcagtc taagaacgga tacgctggtt
     3361 acatcgatgg tggtgcatct caagaagagt tctacaagtt catcaagcct atcctcgaga
     3421 agatggatgg aaccgaggaa ctcctcgtga agctcaatag agaggatctt ctcagaaagc
     3481 agaggacctt cgataacgga tctatccctc atcagatcca cctcggagag ttgcacgcta
     3541 tccttagaag gcaagaggat ttctacccat tcctcaagga taacagggaa aagattgaga
     3601 agattctcac cttcagaatc ccttactacg tgggacctct cgctagagga aactcaagat
     3661 tcgcttggat gaccagaaag tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg
     3721 tggataaggg tgctagtgct cagtctttca tcgagaggat gaccaacttc gataagaacc
     3781 ttccaaacga gaaggtgctc cctaagcact ctttgctcta cgagtacttc accgtgtaca
     3841 acgagttgac caaggttaag tacgtgaccg agggaatgag gaagcctgct tttttgtcag
     3901 gtgagcaaaa gaaggctatc gttgatctct tgttcaagac caacagaaag gtgaccgtga
     3961 agcagctcaa agaggattac ttcaagaaaa tcgagtgctt cgattcagtt gagatttctg
     4021 gtgttgagga taggttcaac gcatctctcg gaacctacca cgatctcctc aagatcatta
     4081 aggataagga tttcttggat aacgaggaaa acgaggatat cttggaggat atcgttctta
     4141 ccctcaccct ctttgaagat agagagatga ttgaagaaag gctcaagacc tacgctcatc
     4201 tcttcgatga taaggtgatg aagcagttga agagaagaag atacactggt tggggaaggc
     4261 tctcaagaaa gctcattaac ggaatcaggg ataagcagtc tggaaagaca atccttgatt
     4321 tcctcaagtc tgatggattc gctaacagaa acttcatgca gctcatccac gatgattctc
     4381 tcacctttaa agaggatatc cagaaggctc aggtttcagg acagggtgat agtctccatg
     4441 agcatatcgc taacctcgct ggatctcctg caatcaagaa gggaatcctc cagactgtga
     4501 aggttgtgga tgagttggtg aaggtgatgg gaaggcataa gcctgagaac atcgtgatcg
     4561 aaatggctag agagaaccag accactcaga agggacagaa gaactctagg gaaaggatga
     4621 agaggatcga ggaaggtatc aaagagcttg gatctcagat cctcaaagag caccctgttg
     4681 agaacactca gctccagaat gagaagctct acctctacta cctccagaac ggaagggata
     4741 tgtatgtgga tcaagagttg gatatcaaca ggctctctga ttacgatgtt gatcatatcg
     4801 tgccacagtc attcttgaag gatgattcta tcgataacaa ggtgctcacc aggtctgata
     4861 agaacagggg taagagtgat aacgtgccaa gtgaagaggt tgtgaagaaa atgaagaact
     4921 attggaggca gctcctcaac gctaagctca tcactcagag aaagttcgat aacttgacta
     4981 aggctgagag gggaggactc tctgaattgg ataaggcagg attcatcaag aggcagcttg
     5041 tggaaaccag gcagatcact aagcacgttg cacagatcct cgattctagg atgaacacca
     5101 agtacgatga gaacgataag ttgatcaggg aagtgaaggt tatcaccctc aagtcaaagc
     5161 tcgtgtctga tttcagaaag gatttccaat tctacaaggt gagggaaatc aacaactacc
     5221 accacgctca cgatgcttac cttaacgctg ttgttggaac cgctctcatc aagaagtatc
     5281 ctaagctcga gtcagagttc gtgtacggtg attacaaggt gtacgatgtg aggaagatga
     5341 tcgctaagtc tgagcaagag atcggaaagg ctaccgctaa gtatttcttc tactctaaca
     5401 tcatgaattt cttcaagacc gagattaccc tcgctaacgg tgagatcaga aagaggccac
     5461 tcatcgagac aaacggtgaa acaggtgaga tcgtgtggga taagggaagg gatttcgcta
     5521 ccgttagaaa ggtgctctct atgccacagg tgaacatcgt taagaaaacc gaggtgcaga
     5581 ccggtggatt ctctaaagag tctatcctcc ctaagaggaa ctctgataag ctcattgcta
     5641 ggaagaagga ttgggaccct aagaaatacg gtggtttcga ttctcctacc gtggcttact
     5701 ctgttctcgt tgtggctaag gttgagaagg gaaagagtaa gaagctcaag tctgttaagg
     5761 aacttctcgg aatcactatc atggaaaggt catctttcga gaagaaccca atcgatttcc
     5821 tcgaggctaa gggatacaaa gaggttaaga aggatctcat catcaagctc ccaaagtact
     5881 cactcttcga actcgagaac ggtagaaaga ggatgctcgc ttctgctggt gagcttcaaa
     5941 agggaaacga gcttgctctc ccatctaagt acgttaactt tctttacctc gcttctcact
     6001 acgagaagtt gaagggatct ccagaagata acgagcagaa gcaacttttc gttgagcagc
     6061 acaagcacta cttggatgag atcatcgagc agatctctga gttctctaaa agggtgatcc
     6121 tcgctgatgc aaacctcgat aaggtgttgt ctgcttacaa caagcacaga gataagccta
     6181 tcagggaaca ggcagagaac atcatccatc tcttcaccct taccaacctc ggtgctcctg
     6241 ctgctttcaa gtacttcgat acaaccatcg ataggaagag atacacctct accaaagaag
     6301 tgctcgatgc taccctcatc catcagtcta tcactggact ctacgagact aggatcgatc
     6361 tctcacagct cggtggtgat tcaagggctg atcctaagaa gaagaggaag gtttgagggg
     6421 ctcacgacga actctaagag ctcaggcctc ccagctttcg tccgtatcat cggtttcgac
     6481 aacgttcgtc aagttcaatg catcagtttc attgcccaca caccagaatc ctactaagtt
     6541 tgagtattat ggcattggaa aagctgtttt cttctatcat ttgttctgct tgtaatttac
     6601 tgtgttcttt cagtttttgt tttcggacat caaaatgcaa atggatggat aagagttaat
     6661 aaatgatatg gtccttttgt tcattctcaa attattatta tctgttgttt ttactttaat
     6721 gggttgaatt taagtaagaa aggaactaac agtgtgatat taaggtgcaa tgttagacat
     6781 ataaaacagt ctttcacctc tctttggtta tgtcttgaat tggtttgttt cttcacttat
     6841 ctgtgtaatc aagtttacta tgagtctatg atcaagtaat tatgcaatca agttaagtac
     6901 agtataggct ttcagctttt tttcttcttc ttcgttcata cagttttttt ttgtttatca
     6961 gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc attcggagtt
     7021 tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg aaccttcaag
     7081 aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc aaaaggcccc
     7141 tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat agtatttctt
     7201 atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt agataagaaa
     7261 acgaagctga gtttatatac agctagagtc gaagtagtga ttggaacaaa gcaccagtgg
     7321 tctagtggta gaatagtacc ctgccacggt acagacccgg gttcgattcc cggctggtgc
     7381 agtcccctcc accccacagt ggttttagag ctagaaatag caagttaaaa taaggctagt
     7441 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttttttttt ctgctttctg
     7501 cccttgccaa tccatatctg aagaagaaaa aaaaacacaa aattacgatt tagatccgat
     7561 ataacaaaat ttgaatcgca cagatcgatc tctttggaga ttctatacct agaaaatgga
     7621 gacgattttc aaatctctgt aaaaattctg gtttcttctt gacggaagaa gacgacgact
     7681 ccaatatttc ggttagtact gaaccggaaa gtttgactgg tgcaaccaat ttaatgtacc
     7741 gtacgtaacg caccaatcgg attttgtatt caatgggcct tatctgtgag cccattaatt
     7801 gatgtgacgg cctaaactaa atccgaacgg tttatttcag cgatccgcga cggtttgtat
     7861 tcagccaata gcaatcaatt atgtagcagt ggtgatcctc gtcaaaccag taaagctaga
     7921 tctggaccgt tgaattggtg caagaaagca catgttgtga tatttttacc cgtacgatta
     7981 gaaaacttga gaaacacatt gataatcgat aaaaaccgtc cgatcatata aatccgcttt
     8041 accatcgttg cctataaatt aatatcaata gccgtacacg cgtgaagact gacaatatta
     8101 tctttttcga attcggagct caagtttgaa attcggagaa gctagagagt tttctgaggt
     8161 acgattcttc gatcctcttt gattttcctg gaaatatttt ttcggtgatc gtgaaactac
     8221 tggaatcgct cgataggtgg tacgaaatta ggcgagatta gtttctattc ttggccatta
     8281 tcttgtttct tcgccgaatg atcttccgga taaagatttt aggttagaga tgaatcgtat
     8341 agctagattt catcaccaga tagtttcttt gtctagaatc tctgaaattc tcgatagttt
     8401 tcacatgtgt aaatagattg ttcttattcg gcgattgttg attagggttt tgattttctt
     8461 gattatgcga ttgcaattag ggattttctt tggttttgtg ttgatcttac gatacattcc
     8521 ggcaattgaa tacgtatgga tctaaatctt gttaatttgt tgaacaggac gatggtcccc
     8581 tccaccccac agtgggggca ccaagagccg agaagaagcc cgccgagaaa aaaaccgccg
     8641 ctgagaggcc ggtggaagag aacaaggctg ctgaaaaggc tccggcggag aagaaaccca
     8701 aggcggggaa gaaattgcca ccaaaggaag ccggtgacaa gaagaagaag cgatctaaga
     8761 aaaacgtcga aacctacaag atctacatct tcaaggtgct gaagcaggtt catccagata
     8821 tcggaatttc aagcaaagcc atggggatta tgaacagttt catcaacgac atattcgaga
     8881 agctagctca ggaatcttcg aaactcgcta ggtacaacaa gaagccgacc attacatctc
     8941 gcgagatcca gacggctgtg agactcgtcc tccccggtga actcgctaaa cacgccgtct
     9001 ctgaaggtac caaggcggtt accaagttta ctagctccat ggtgagcaag ggcgaggagc
     9061 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     9121 tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
     9181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
     9241 gcgtggcctg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     9301 ccatgcccga aggctacgtc caggagcgca ccatctcttt caaggacgac ggtacctaca
     9361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     9421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
     9481 gccacaacgt ctatatcacg gccgacaagc agaagaacgg catcaaggct aacttcaaga
     9541 tccgccacaa cgttgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     9601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat cagtccgccc
     9661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     9721 ccgggattac acatggcatg gacgagctgt acaagtaatc aggtggatcc tagataacct
     9781 ttaccttcat tccctgtaaa ttggtaccct gcatctcgtc tctgttatgc ttaagaagtt
     9841 caatgtttcg tttcatgtaa aactttggtg gtttgtgttt tggggccttg tataatccct
     9901 gatgaataag tgttctacta tgtttccgtt cctgttatct ctttctttct aatgacaagt
     9961 cgaacttctt ctttatcatc gcttcgtttt tattatctgt gcttcttttg tttaatacgc
    10021 ctgcaaagtg actcgactct gtttagtgca gttctgcgaa acttgtaaat agtccaattg
    10081 ttggcctcta gtaatagatg tagcgaaagt gttgagctgt tgggttctaa ggatggcttg
    10141 aacatgttaa tcttttaggt tctgagtatg atgaacattc gttgttgcta agaaatgcct
    10201 gtaatgtccc acaaatgtag aaaatggttc gtacctttgt ccaagcattg atatgtctga
    10261 tgagaggaaa ctgcaagata ctgagcttgg tttaacgaag gagaggcagt ttcttccttc
    10321 caaagcattt catttgacaa tgccttgatc atcttaagta gagtttctgt tgtggaaagt
    10381 ttgaaacttt gaagaaacga ctctcaagta aattgatgat cacaagtgaa agtgtatgtt
    10441 actaactatt gaaaccaaat taacataggg tttttattta aataaaagtt taaccttctt
    10501 tttaaaaaat tgttcatagt gtcatgtcag aacaagagct acaaatcaca catagcatgc
    10561 ataagcggag ctatgatgag tggtattgtt ttgttcgtca cttgtcactc ttttccaaca
    10621 cataatcccg acaacaacgt aagagcatct ctctctctcc acacacactc atgcatgcat
    10681 gcattcttac acgtgattgc catgcaaatc tcctttctca cctataaata caaaccaacc
    10741 cttcactaca ctcttcactc aaaccaaaac aagaaaacat acacaaatag caaaacaagg
    10801 gcgaattctc aacacaacat atacaaaaca aacgaatctc aagcaatcaa gcattctact
    10861 tctattgcag caatttaaat catttctttt aaagcaaaag caattttctg aaaattttca
    10921 ccatttacga acgatagcca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
    10981 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
    11041 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
    11101 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
    11161 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
    11221 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
    11281 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
    11341 cccctccgac ggccccgtaa tgcagaagaa gacgatgggc tgggaggcct cctccgagcg
    11401 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
    11461 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
    11521 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
    11581 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
    11641 gtacaagtag cttggctgca ggtcgacctg caggcatgcc cgctgaaatc accagtctct
    11701 ctctacaaat ctatctctct ctataataat gtgtgagtag ttcccagata agggaattag
    11761 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
    11821 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccaggggtac
    11881 cgtatgatct agggaagttc ctattccgaa gttcctattc tctgaaaagt ataggaactt
    11941 ctttgcgtat tgggcgctct tggccttttt ggccaccggt cgtacggtta aaaccacccc
    12001 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
    12061 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
    12121 caccactcga tacaggcagc ccatcagtcc actagagacg ctcaccgggc tggttgccct
    12181 cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc
    12241 gtgtgcgaga caccgcagcc gccggcgttg tggatacctc gcggaaaact tggccctcac
    12301 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
    12361 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
    12421 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
    12481 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
    12541 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
    12601 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
    12661 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
    12721 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
    12781 ggcccgctct cgcgttggca gcatcaccca taattgtggt ttcaaaatcg gctccgtcga
    12841 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaaa
    12901 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag ggaaggtgcg
    12961 aataagcggg gaaattcttc tcggctgact cagtcatttc atttcttcat gtttgagccg
    13021 attttttctc ccgtaaatgc cttgaatcag cctatttaga ccgtttcttc gccatttaag
    13081 gcgttatccc cagtttttag tgagatctct cccactgacg tatcatttgg tccgcccgaa
    13141 acaggttggc cagcgtgaat aacatcgcca gttggttatc gtttttcagc aaccccttgt
    13201 atctggcttt cacgaagccg aactgtcgct tgatgatgcg aaatgggtgc tccaccctgg
    13261 cccggatgct ggctttcatg tattcgatgt tgatggccgt tttgttcttg cgtggatgct
    13321 gtttcaaggt tcttaccttg ccggggcgct cggcgatcag ccagtccaca tccacctcgg
    13381 ccagctcctc gcgctgtggc gccccttggt agccggcatc ggctgagaca aattgctcct
    13441 ctccatgcag cagattaccc agctgattga ggtcatgctc gttggccgcg gtggtgacca
    13501 ggctgtgggt caggccactc ttggcatcga caccaatgtg ggccttcatg ccaaagtgcc
    13561 actgattgcc tttcttggtc tgatgcatct ccggatcgcg ttgctgctct ttgttcttgg
    13621 tcgagctggg tgcctcaatg atggtggcat cgaccaaggt gccttgagtc atcatgacgc
    13681 ctgcttcggc cagccagcga ttgatggtct tgaacaattg gcgggccagt tgatgctgct
    13741 ccagcaggtg gcggaaattc atgatggtgg tgcggtccgg caaggcgcta tccagggata
    13801 accgggcaaa cagacgcatg gaggcgattt cgtacagagc atcttccatc gcgccatcgc
    13861 tcaggttgta ccaatgctgc atgcagtgaa tgcgtagcat ggtttccagc ggataaggtc
    13921 gccggccatt accagccttg gggtaaaacg gctcgatgac ttccaccatg ttttgccatg
    13981 gcagaatctg ctccatgcgg gacaagaaaa tctcttttct ggtctgacgg cgcttactgc
    14041 tgaattcact gtcggcgaag gtaagttgat gactcatgat gaaccctgtt ctatggctcc
    14101 agatgacaaa catgatctca tatcagggac ttgttcgcac cttccttagc ttcttggggt
    14161 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
    14221 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
    14281 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
    14341 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
    14401 aaggaaagct gcctgttcca aaggtcctgc accttgaacg gcatgatggc tggagcaatc
    14461 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
    14521 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
    14581 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
    14641 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacacccca tttaaagatc
    14701 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
    14761 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
    14821 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
    14881 tcagggagga tattggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
    14941 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
    15001 atgtggcgca acgatgctgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt
    15061 tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg
    15121 cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac
    15181 ttcattgccg ataaggtgga ttatctggac accaaggcac caggcggatc aaatcaggaa
    15241 taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg
    15301 acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat
    15361 gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc
    15421 ggctcgatgg cccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc
    15481 cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg
    15541 gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga
    15601 aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaagc cgcgttgctg
    15661 aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg
    15721 ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc
    15781 aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac
    15841 gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag
    15901 caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac
    15961 gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa
    16021 tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg
    16081 gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt
    16141 tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa
    16201 ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc
    16261 agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg
    16321 gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga
    16381 ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag
//
