LOCUS       sequence-426239-        6641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human WDR19 fragment M1 to G636. N-terminal 6X 
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR19:1-636
FEATURES             Location/Qualifiers
     source          1..6641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1188..1322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1225..1244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1279..1298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1351..1516
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1700..2154
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1787..1806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1996..2017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2180..2284
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2285..2353,2354..3145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2503..2522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3316..3904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3805..3824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4058..4075
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4209..4433
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4500..5033)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5203..5208,5209..5225,5226..5231))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5583..5674
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5599..5616
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5733..5750
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5763..5783
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cttaacaaag ttgctacatg tggagataac tgcattaaaa tccaagactt ggttgactta
       61 aaagacatgt atgttatact caacctggat gaggaaaata aaggattggg taccttgtcc
      121 tggactgatg atggccagtt gctagcactc tctacccaaa ggggctcact tcatgttttc
      181 ctgaccaagc ttcccatact tggggatgcc tgcagcacaa ggattgccta tctcacctcc
      241 ctccttgaag tcaccgtagc caaccctgtt gaaggagagc taccaatcac agtttctgtt
      301 gatgtggaac ccaactttgt ggcagtaggt ctttatcatc tggctgtagg aatgaataat
      361 cgagcttggt tttatgtcct tggagaaaat gctgtgaaaa aattgaaaga tatggagtat
      421 ctgggaacag tagccagtat ttgccttcat tctgactatg ctgctgcact ttttgaaggc
      481 aaagtccagt tacatttgat agaaagcgaa atcttggatg ctcaagaaga acgtgagact
      541 cggcttttcc cagcagtgga tgataagtgc cgtatcttat gccatgcctt aactagtgat
      601 ttcctcatct atggtacaga tactggtgtc gttcagtatt tctacattga agactggcaa
      661 ttcgttaatg attatcgaca tcctgtcagt gtgaaaaaga tttttcccga cccaaatggg
      721 accagattag ttttcattga tgaaaaaagt gatggatttg tttactgtcc agtcaatgac
      781 gctacctatg agattccaga tttttcacca accattaaag gtgttctttg ggaaaactgg
      841 ccaatggata aaggtgtatt tattgcttat gatgatgata aggtgtacac ttatgtcttt
      901 cacaaggaca ctatacaagg agccaaggtt attttggctg gtagcaccaa agttcctttt
      961 gctcataaac ctttgctgct atataatgga gagctgacct gccaaacaca gagtggaaaa
     1021 gtaaacaaca tctaccttag cacccatggc tgatgacgaa gcttgtcgag aagtactaga
     1081 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1141 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1321 tctgatcact gatatcgcct aggagatccg aaccagataa gtgaaatcta gttccaaact
     1381 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     1441 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     1501 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
     1561 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
     1621 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
     1681 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1741 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1801 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1861 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1921 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1981 tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2041 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2101 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     2161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2281 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2341 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2401 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2461 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2521 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2581 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2641 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2701 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2761 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2821 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2881 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2941 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3001 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3061 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3121 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3181 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3241 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3301 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3361 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3421 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3481 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3541 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3601 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3661 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3721 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3781 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3841 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3901 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3961 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4021 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4081 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4141 agaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
     4201 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     4261 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     4321 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
     4381 tgcccgtcgt attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt
     4441 tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt
     4501 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt
     4561 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca
     4621 ccaagcgcgt tggcctcatg cttgagcaga ttgatgagcg cggtggcaat gccctgcctc
     4681 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac
     4741 ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc
     4801 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg
     4861 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat
     4921 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac
     4981 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5041 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     5101 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     5161 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     5221 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     5281 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     5341 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     5401 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     5461 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     5521 ttcgcccagg actctagcta tagttctagt ggttggctac gtatactccg gaatattaat
     5581 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
     5641 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
     5701 catcgggcgc ggatctcggt ccgaaaacca tgcatcatca tcatcatcac agcagcggca
     5761 gagaaaactt gtatttccag ggcatgaagc gtattttctc actgctagaa aagacttggc
     5821 ttggcgcacc aatacagttt gcctggcaaa aaacatcagg aaactacctt gcagtaacag
     5881 gagctgatta tattgtgaaa atctttgatc gccatggtca aaaaagaagt gaaattaact
     5941 tacctggtaa ctgtgttgcc atggattggg ataaagatgg agatgtccta gcagtgattg
     6001 ctgagaaatc tagctgcatt tatctttggg atgccaacac aaataagacc agccagttag
     6061 acaatggcat gagggatcaa atgtctttcc ttctttggtc aaaagttgga agtttcctgg
     6121 ctgttggaac tgttaaagga aatttgctta tttataatca tcagacatct cgaaagattc
     6181 ctgtccttgg aaaacatact aagagaatca cttgtggatg ttggaatgca gaaaatctgc
     6241 ttgctttagg tggtgaagat aaaatgatta cagttagtaa tcaggaaggt gacacgataa
     6301 gacagacaca agtgagatca gagcctagca acatgcagtt tttcttgatg aagatggatg
     6361 accgaacctc tgctgctgaa agcatgataa gtgtggtgct tggcaagaaa actttgtttt
     6421 ttttaaatct gaatgaacca gataacccag ctgatcttga atttcagcag gactttggca
     6481 acattgtctg ctataattgg tatggtgatg gccgcatcat gattggtttt tcatgtggac
     6541 attttgtggt catttctact catactggag agcttggtca agagatattt caggctcgta
     6601 accataaaga taatctaacc agcattgcag tatcacagac t
//
