LOCUS       sequence-42624-e        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, 
            Howarth M
  TITLE     Peptide tag forming a rapid covalent bond to a protein, through 
            engineering a bacterial adhesin.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 
            24.
  PUBMED    22366317
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-20"; volume: "109(12)"; pages: 
            "E690-7. Epub 2012 Feb 24"
COMMENT     Sequence Label: pET28a-SpyTagMBP
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             111..149
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     gap             1167..1178
                     /estimated_length=12
     gap             1211..1223
                     /estimated_length=13
     gap             1235..1246
                     /estimated_length=12
     gap             1250..1259
                     /estimated_length=10
ORIGIN
        1 tnnnnctnnn ntaattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggga gcccacatcg
      121 tgatggtgga cgcctacaag ccgacgaagg gtagtggtga aagtggtaaa atcgaagaag
      181 gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta
      241 agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag
      301 agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg
      361 accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt
      421 tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg
      481 cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc
      541 cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga
      601 gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg
      661 ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata
      721 acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga
      781 atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga
      841 ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa
      901 cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcnn
      961 nattaacgcc gccagtccga acaaagagct ggcaaagagt tcctcgaaac tatctgctga
     1021 ctgatgaagg tctggancgg tnanaagacn aacnctgggn gccgtancnn tgagtntacn
     1081 aggananttg gcnaagatcc acgtntgcnc nctatnnaaa nncccnnaan gtgaaatcat
     1141 ncnnncatcc gcnnanntcn nnttctnnnn nnnnnnnncn nngnnatnan nnnnccnnng
     1201 nnnnnnanng nnnnnnnnnn nnnannnncn nactnnnnnn nnnnnnaaan nnnnnnnnng
//
