LOCUS       sequence-426240-        5693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human IFT172 fragment M1 to S320. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_IFT172:1-320
FEATURES             Location/Qualifiers
     source          1..5693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    130..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(167..186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     221..240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  293..458
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      642..1096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(729..748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     938..959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1122..1226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1227..1295,1296..2087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1445..1464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2258..2846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2747..2766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3000..3017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3151..3375
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3442..3975)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4145..4150,4151..4167,4168..4173))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        4525..4616
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4541..4558
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4675..4692
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4705..4725
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
       61 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      121 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      241 aatgtatctt atcatgtctg gatctgatca ctgatatcgc ctaggagatc cgaaccagat
      301 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
      361 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
      421 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
      481 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
      541 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
      601 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      781 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      901 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      961 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     1021 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     1081 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1141 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1201 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1261 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1321 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1381 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1441 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1501 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1561 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1621 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1681 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1741 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1801 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1861 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1921 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1981 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2041 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2101 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2161 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2221 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2281 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2341 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2401 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2461 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2521 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2581 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2641 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2701 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2761 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2821 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2881 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2941 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3001 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     3061 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     3121 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     3181 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     3241 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     3301 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     3361 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     3421 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     3481 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3541 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag
     3601 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3661 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3721 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3781 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3841 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3901 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3961 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     4021 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     4081 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     4141 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     4201 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
     4261 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
     4321 cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg
     4381 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
     4441 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct
     4501 acgtatactc cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa
     4561 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc
     4621 cggattattc ataccgtccc accatcgggc gcggatctcg gtccgaaaac catgcatcat
     4681 catcatcatc acagcagcgg cagagaaaac ttgtatttcc agggcatgca cttgaagcac
     4741 ctgaggaccc tgctgagccc tcaggatgga gctgcaaagg tgacctgcat ggcttggtcc
     4801 cagaacaatg ccaaatttgc tgtctgcaca gtggaccgag tggtcttgct gtatgatgaa
     4861 catggagaac ggagagataa attctccacc aaaccagctg acatgaagta tggcaggaag
     4921 agctatatgg tgaagggcat ggctttttct cctgattcca ctaaaattgc cataggacag
     4981 actgacaaca tcatctatgt ctacaagatt ggagaagatt ggggtgacaa gaaagtcatc
     5041 tgcaacaagt tcatccagac gagtgctgtc acttgtctgc aatggccggc agaatacatc
     5101 attgtctttg gactggctga agggaaggtt cgtttagcaa acaccaaaac taataaatca
     5161 tctaccatct atgggacaga gtcttacgtg gtgtccctga caacaaattg ctctgggaaa
     5221 ggaattctct ctggtcatgc agatggtacc atcgttaggt atttctttga tgatgaaggc
     5281 tctggagagt cacaggggaa gttggttaac cacccgtgtc caccctatgc cttggcatgg
     5341 gcaaccaata gcatcgtggc tgcaggctgt gatcggaaaa ttgtagccta tggaaaagaa
     5401 ggtcacatgc tacaaacttt tgattatagc cgtgaccctc aggagcggga gttcaccaca
     5461 gctgtatcaa gtcctggggg ccagtctgtt gtgctaggaa gttatgacag gcttcgggtg
     5521 ttcaactgga tccctcgaag aagcatctgg gaagaggcaa agcccaagga gattaccaat
     5581 ttatacacca tcactgcctt ggcctggaag cgggatggct cacggctctg tgtgggcaca
     5641 ctatgtggtg gggtggaaca gtttgactgc tgcctccgaa ggagttgatg acg
//
