LOCUS       sequence-426243-        5741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X 
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_TBL2:70-405
FEATURES             Location/Qualifiers
     source          1..5741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  21..245
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(312..845)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1015..1020,1021..1037,1038..1043))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1395..1486
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1411..1428
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1545..1562
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1575..1595
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2741..2875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2778..2797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2832..2851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2904..3069
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3253..3707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3340..3359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3549..3570
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3733..3837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3838..3906,3907..4698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4056..4075)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4869..5457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5358..5377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5611..5628
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
       61 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
      121 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
      181 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta
      241 tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
      301 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
      361 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
      421 atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag cgcggtggca
      481 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
      541 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
      601 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
      661 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
      721 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
      781 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat
      841 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
      901 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
      961 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     1021 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     1081 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
     1141 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
     1201 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
     1261 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
     1321 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc
     1381 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     1441 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     1501 ataccgtccc accatcgggc gcggatctcg gtccgaaaac catgcatcat catcatcatc
     1561 acagcagcgg cagagaaaac ttgtatttcc agggcgagaa gcctcaacaa cacaacttca
     1621 cccaccgcct cctggctgca gctctgaaga gccacagcgg gaacatatct tgcatggact
     1681 ttagcagcaa tggcaaatac ctggctacct gtgcagatga tcgcaccatc cgcatctgga
     1741 gcaccaagga cttcctgcag cgagagcacc gcagcatgag agccaacgtg gagctggacc
     1801 acgccaccct ggtgcgcttc agccctgact gcagagcctt catcgtctgg ctggccaacg
     1861 gggacaccct ccgtgtcttc aagatgacca agcgggagga tgggggctac accttcacag
     1921 ccaccccaga ggacttccct aaaaagcaca aggcgcctgt catcgacatt ggcattgcta
     1981 acacagggaa gtttatcatg actgcctcca gtgacaccac tgtcctcatc tggagcctga
     2041 agggtcaagt gctgtctacc atcaacacca accagatgaa caacacacac gctgctgtat
     2101 ctccctgtgg cagatttgta gcctcgtgtg gcttcacccc agatgtgaag gtttgggaag
     2161 tctgctttgg aaagaagggg gagttccagg aggtggtgcg agccttcgaa ctaaagggcc
     2221 actccgcggc tgtgcactcg tttgctttct ccaacgactc acggaggatg gcttctgtct
     2281 ccaaggatgg tacatggaaa ctgtgggaca cagatgtgga atacaagaag aagcaggacc
     2341 cctacttgct gaagacaggc cgctttgaag aggcggcggg tgccgcgccg tgccgcctgg
     2401 ccctctcccc caacgcccag gtcttggcct tggccagtgg cagtagtatt catctctaca
     2461 atacccggcg gggcgagaag gaggagtgct ttgagcgggt ccatggcgag tgtatcgcca
     2521 acttgtcctt tgacatcact ggccgctttc tggcctcctg tggggaccgg gcggtgcggc
     2581 tgtttcacaa cactcctggc cactgatgac gaagcttgtc gagaagtact agaggatcat
     2641 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2701 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc
     2881 actgatatcg cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt
     2941 catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact
     3001 cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca actattttgt
     3061 ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc
     3121 catgtgacaa accgtcatct tcggctactt tttctctgtc acagaatgaa aatttttctg
     3181 tcatctcttc gttattaatg tttgtaattg actgaatatc aacgcttatt tgcagcctga
     3241 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3301 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3541 tcttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3601 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3661 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     3721 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3781 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3841 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3901 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3961 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4021 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4081 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4141 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4201 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4261 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4321 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4381 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4441 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4501 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4561 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4621 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4681 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4741 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4801 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4861 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4921 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4981 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5041 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5101 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5161 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5221 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5281 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5341 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5401 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5461 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5521 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5581 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     5641 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagaccag
     5701 ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa t
//
