LOCUS       sequence-426244-        5843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X 
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_UTP15:1-370
FEATURES             Location/Qualifiers
     source          1..5843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(261..794)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(964..969,970..986,987..992))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1344..1435
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1360..1377
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1494..1511
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1524..1544
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2792..2926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2829..2848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2883..2902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2955..3120
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3304..3758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3391..3410)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3600..3621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3784..3888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3889..3957,3958..4749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4107..4126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4920..5508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5409..5428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5662..5679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  join(5813..5843,1..194)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa
       61 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
      121 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg
      181 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
      241 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
      301 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
      361 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgagcag attgatgagc
      421 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
      481 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
      541 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
      601 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
      661 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
      721 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      781 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
      841 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
      901 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
      961 tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac
     1021 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     1081 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     1141 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     1201 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     1261 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
     1321 cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa
     1381 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
     1441 ggattattca taccgtccca ccatcgggcg cggatctcgg tccgaaaacc atgcatcatc
     1501 atcatcatca cagcagcggc agagaaaact tgtatttcca gggcatggct ggttataagc
     1561 ctgtagctat tcagacatat cctatacttg gtgaaaaaat cacccaagat acactgtact
     1621 ggaacaacta taagacccct gttcagatta aggaatttgg tgcagtttca aaagtagact
     1681 tttctcctca gcctccatat aattatgctg tcacagcttc ctcaagaatt cacatttatg
     1741 gccgatactc ccaagaacct ataaaaacct tttctcgatt taaagacaca gcatactgtg
     1801 ctacttttcg acaagatggt agattgcttg tggctggcag tgaagatggt ggagttcaac
     1861 tttttgatat aagtgggagg gctcccctca ggcagtttga aggccataca aaagcagttc
     1921 atacagtaga ttttacagct gacaaatatc acgtggtctc tggggctgat gattatacag
     1981 ttaaattatg ggatattcca aactccaaag aaattttgac atttaaagaa cactctgatt
     2041 atgtgaggtg tggatgtgct agcaaactta atccggatct ctttataaca ggatcatatg
     2101 atcatactgt gaagatgttt gatgcacgaa cgagtgagag tgttctctcc gttgagcatg
     2161 ggcagccagt ggagagtgtc ctacttttcc cctctggagg tcttctggtg tcagcaggag
     2221 gtcgttatgt taaagtctgg gacatgttaa aaggaggaca attgctagta tctttgaaaa
     2281 atcatcacaa aaccgtgaca tgtttatgtc taagcagctc tggacagagg ttactctctg
     2341 gctcactgga taggaaggtg aaagtataca gcacaacttc ctacaaagta gtccacagtt
     2401 ttgattatgc agcttcaatt ttgagtcttg cccttgcaca tgaagatgag acaatagttg
     2461 taggaatgac caatggaata ctgagtgtta aacatcggaa atctgaagca aagaaggaat
     2521 cacttcccag aagaagaagg cctgcatatc gaacctttat taaaggaaaa aattacatga
     2581 agcaacggga tgacattttg attaacaggc cagcaaagaa gcacctagaa ttgtatgaca
     2641 gggatctgaa acattgatga cgaagcttgt cgagaagtac tagaggatca taatcagcca
     2701 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2761 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2821 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2881 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgatatc
     2941 gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa
     3001 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa
     3061 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca
     3121 gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca
     3181 aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt
     3241 cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat
     3301 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3361 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3421 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3481 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3541 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag
     3601 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3661 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3721 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     3781 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3841 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3901 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3961 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4021 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4081 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4141 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4201 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4261 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4321 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4381 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4441 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4501 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4561 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4621 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4681 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4741 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4801 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4861 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4921 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4981 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5041 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5101 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5161 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5221 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5281 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5341 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5401 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5461 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5521 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5581 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5641 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     5701 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     5761 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     5821 gacaataaag tcttaaactg aac
//
