LOCUS       sequence-426247-        6157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human DCAF12. N-terminal 6X His 
            tag, biotin acceptor peptide tag and TEV cleavage site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-avi-TEV-LIC_DCAF12:1-453
FEATURES             Location/Qualifiers
     source          1..6157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    946..1080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(983..1002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1037..1056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1109..1274
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1458..1912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1545..1564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1754..1775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1938..2042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2043..2111,2112..2903)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2261..2280)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3074..3662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3563..3582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3816..3833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3967..4191
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4258..4791)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4961..4966,4967..4983,4984..4989))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5341..5432
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5357..5374
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5490..5507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5520..5564
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5586..5606
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggactggatc ttttccatcg catggatcag cgacactatg gcagtgtctg gctcacgtga
       61 tggttctatg ggactctggg aggtgacaga tgatgttttg accaaaagtg atgcgagaca
      121 caatgtgtca cgggtccctg tgtatgcaca catcactcac aaggccttaa aggacatccc
      181 caaagaagac acaaaccctg acaactgcaa ggttcgggct ctggccttca acaacaagaa
      241 caaggaactg ggagcagtgt ctctggatgg ctactttcat ctctggaagg ctgaaaatac
      301 actatctaag ctcctctcca ccaaactgcc atattgccgt gagaatgtgt gtctggctta
      361 tggtagtgaa tggtcagttt atgcagtggg ctcccaagct catgtctcct tcttggatcc
      421 acggcagcca tcatacaacg tcaagtctgt ctgttccagg gagcgaggca gtggaatccg
      481 gtcagtgagt ttctacgagc acatcatcac tgtgggaaca gggcagggct ccctgctgtt
      541 ctatgacatc cgagctcaga gatttctgga agagaggctc tcagcttgtt atgggtccaa
      601 gcccagacta gcaggggaga atctgaaact aaccactggc aaaggctggc tgaatcatga
      661 tgaaacctgg aggaattact tttcagacat tgacttcttc cccaatgctg tttacaccca
      721 ctgctacgac tcgtctggaa cgaaactctt tgtggcagga ggtcccctcc cttcagggct
      781 ccatggaaac tatgctgggc tctggagttg atgacgaagc ttgtcgagaa gtactagagg
      841 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      901 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      961 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1021 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     1081 tgatcactga tatcgcctag gagatccgaa ccagataagt gaaatctagt tccaaactat
     1141 tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg
     1201 tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat
     1261 tttgtccgcc cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc
     1321 aactccatgt gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt
     1381 ttctgtcatc tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag
     1441 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1501 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1561 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1621 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1681 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1741 cacgttctta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     1801 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     1861 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     1921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2281 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3781 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3841 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag
     3901 accagccgcg taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta
     3961 agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa
     4021 taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa
     4081 aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg
     4141 cccgtcgtat taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg
     4201 tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta
     4261 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     4321 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     4381 aagcgcgttg gcctcatgct tgagcagatt gatgagcgcg gtggcaatgc cctgcctccg
     4441 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt
     4501 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     4561 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     4621 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     4681 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     4741 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     4801 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
     4861 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     4921 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
     4981 ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
     5041 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt
     5101 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac
     5161 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt
     5221 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt
     5281 cgcccaggac tctagctata gttctagtgg ttggctacgt atactccgga atattaatag
     5341 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
     5401 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
     5461 tcgggcgcgg atctcggtcc gaaaccatgc atcatcacca tcaccatgaa ttcatgagcg
     5521 gcctgaacga tatttttgaa gcgcagaaaa ttgaatggca tgaaggcagc gctggaggtt
     5581 caggtgaaaa cttgtatttc cagggcatgg cccggaaagt agttagcagg aagcggaaag
     5641 cgcccgcctc gccgggagct gggagcgacg ctcagggccc gcagtttggc tgggatcact
     5701 cgcttcacaa aaggaaaaga cttcctcctg tgaagagatc cttagtatac tacttgaaga
     5761 accgggaagt caggctacag aatgaaacca gctactctcg agtgttgcat ggttatgcag
     5821 cacagcaact tcccagtctc ctgaaggaga gagagtttca ccttgggacc cttaataaag
     5881 tgtttgcatc tcagtggttg aatcataggc aagtggtgtg tggcacaaaa tgcaacacgc
     5941 tatttgtcgt agatgtccag acaagccaga tcaccaagat ccccattctg aaagaccagg
     6001 agcctggagg tgtgacccag cagggctgtg gtatccatgc catcgagctg aatccttcta
     6061 gaacactgct agccactgga ggagacaacc ccaacagtct tgccatctat cgactaccta
     6121 cgctggatcc tgtgtgtgta ggagatgatg gacacaa
//
