LOCUS       sequence-42625-e        1256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, 
            Howarth M
  TITLE     Peptide tag forming a rapid covalent bond to a protein, through 
            engineering a bacterial adhesin.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 
            24.
  PUBMED    22366317
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-20"; volume: "109(12)"; pages: 
            "E690-7. Epub 2012 Feb 24"
COMMENT     Sequence Label: pET28a-SpyTagMBP
FEATURES             Location/Qualifiers
     source          1..1256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(88..111)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     gap             1239..1255
                     /estimated_length=17
ORIGIN
        1 cnttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttt tacgagctcg aattagtctg cgcgtctttc agggcttcat cgacagtctg
      121 acgaccgctg gcggcgttga tcaccgcagt acgcacggca taccagaaag cggacatctg
      181 cgggatgttc ggcatgattt cacctttctg ggcgttttcc atagtggcgg caatacgtgg
      241 atctttcgcc aactcttcct cgtaagactt cagcgctacg gcacccagcg gtttgtcttt
      301 attaaccgct tccagacctt catcagtcag cagatagttt tcgaggaact cttttgccag
      361 ctctttgttc ggactggcgg cgttaatacc tgcgctcagc acgccaacga acggtttgga
      421 tggttgaccc ttgaaggtcg gcagtaccgt tacaccataa ttcactttgc tggtgtcgat
      481 gttggaccat gcccacgggc cgttgatggt catcgctgtt tcgcctttat taaaggcagc
      541 ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg tttttaatca ggtcaaccag
      601 gaaggtcaga cccgctttcg cgccagcgtt atccacgccc acgtctttaa tgtcgtactt
      661 gccgttttca tacttgaacg cataaccccc gtcagcagca atcagcggcc aggtgaagta
      721 cggttcttgc aggttgaaca tcagcgcgct cttacctttc gctttcagtt ctttatccag
      781 cgccgggatc tcttcccagg tttttggcgg gttcggcagc agatctttgt tataaatcag
      841 cgataacgct tcaacagcga tcgggtaagc aatcagcttg ccgttgtaac gtacggcatc
      901 ccaggtaaac ggatacagct tgtcctnnac gctttgtccg gggtgatttc agccaacagg
      961 ccagattgag cgtagccacc aaagcggtcg tgtgcccaga anatnatgtc nggccatcgc
     1021 cagttgccgc aacctgngga atttctcttc ngttatccgg atgctcaacg ngactttaat
     1081 ncnntatctt tctcgaattt cntaccgact nancnnnacg tnnnnnnntt nnngncntnn
     1141 ancnnantnn nnttnactnn nncgatttac nnttnncnnn naccnnnnng nnnnnagnnn
     1201 ncnccatnnc nannnnngnn ncnnnnngnc nnncnnngnn nnnnnnnnnn nnnnnc
//
