LOCUS       sequence-426252-       17991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector expressing the estradiol-inducible Lineage Tracing 
            system. XVE expression driven by GL2 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 17991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: ipGL2Cas
FEATURES             Location/Qualifiers
     source          1..17991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    214..238
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     291..313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             4230..4481
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             4497..4730
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             4740..5675
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4788..5681
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        6478..6524
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             6546..10649
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6546..10649
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10662..10682
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(11654..11656,11657..11659,11660..12373)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11699..11720)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11960..11979)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12307..12328
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    12617..12664
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     misc_feature    12776..12800
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      12880..13511
                     /label=oriV
                     /note="incP origin of replication"
     CDS             14922..15716
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             16015..17163
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(17232..17249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17403..17991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17483..17502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt ggatctcctg
      121 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
      181 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
      241 cactgatagt ttaaactgaa ggcgggaaac gacaatctgc tagtggatct cccagtcacg
      301 acgttgtaaa acgggcgccc cgcggaaagc ttgctagcca attggggccc aacgttctcg
      361 agtttttcta gaaggccttg gatccacccg ggagaattcg tcgactttgc ggccgcatcg
      421 atactgcagg agctcggtac cttttactag tgatatcgat catgagcgac ctcaccccta
      481 tgtgttttat gtttttttag atccaacaaa atccaaatcc ttttttttcc ctcggccacc
      541 ggcggtacaa acggcgtcgt ttcgcggatt agaaaaacgt tctgtcttga tatcatttac
      601 ttcattctaa gcggctttgg tctgaatttt ttatatacaa ggcctgtctc cgtttttgta
      661 aagggaaaac agtaggatcc attttagcct ctgtaagtaa caatattggg cccctaaaag
      721 cccacccatt ttggggccag caaaccaagg caccctcggt tccgcacgct cgctaagacg
      781 ctaacctatg catatgttga tatgtttttt ctcttccttt tggtatgaat cttgatttgt
      841 tttgatactc atgatgtaca ttcgtattct cttacgtatt gtaaaccatc ctatttcaga
      901 tcacgattat atctttacat ttacattttt catttttatt tctgtttgaa tgttacaatt
      961 tactagtaga gttattcatt aaaatactac aactggtata cagaaatgta atttgagtga
     1021 taaattatat gaaataatta agtaatatat gtgatattta tggatccaaa caaaaactaa
     1081 ttactggttc attttctatt ttagatgtaa gcaaaatgtg taagattcaa ggtatatata
     1141 tatcccaata tacgtatata tgtggtactc actagctagt agctctctca caactgtgtc
     1201 ttttggtttt catcagctga tcctctccaa ctaactatcc atcttttgtt ttgcggttgg
     1261 acttggaggt accaagaata ttagcaacgt acgactcgta tggtatcatt tcctttgtac
     1321 aaaaagtgaa tatcaaaatg cattgtatta attatatata agttagtata tggagttagt
     1381 tgtcctcact gtctttatct ggcgcaatct cctatgccat cattccctct tcacacgtac
     1441 gtgtgcacac tcgatgtcac atttgtataa acacgtttgc ttttagcgtg agatcatcac
     1501 catattccat ttttggtggg tcagttctct ttctagatag ttatttgtaa ggacgtgaat
     1561 taaaagggat cgtcgtcact tgttgagata aaagaaaaga tatatggtca gtttctgcat
     1621 cttggaatca acttaagggt tgtcttaatt aattttgata gaccctactt taaaaattaa
     1681 ttagttgctt tcattggccc tcaataagaa aagccaaaaa agaaagaaga ctggtcttgg
     1741 aagtttgcca acacgggtaa tagattaatg gtgaaaaggg cgaatttttt tacccaaaac
     1801 cctaattaag tagaagtatt aatcgagagc aaaaaagaga gagagagtca gtagccaaaa
     1861 ggaatgaatg gaagaaagaa aaaggaatct ctataggcag catatattca agtaattaat
     1921 taaagtagat agatagagca aaaggagagg ttaggaggca ttaattaatt atttaagagc
     1981 atgtggtgaa tgtaaatgtt tatggttgct tccctctcta tacattatgt atctaccttt
     2041 cctaactaac aattccctag gccgtacgac gactaacaaa gaaaaaaaca aaagaaactg
     2101 ataaagcttt tgaattgtag ataaatcatc tgctacagtt ataccattat atatcttatt
     2161 aaagacctaa gtttccttca ctatacgtct tcgtccattt acgtacgtat tatacggacg
     2221 gtttaagcta ctatatctat attgttaaca atgtaactgt tgagatatat cttgcaataa
     2281 tatgtcatgg tgtatgcata cgataatatg aatcaatgtt tgaaatcttg acgtgcccgt
     2341 gatacaataa gatgatcaaa atttcaaatt ttgtcaaata ttaaaacaac atacacatac
     2401 acatgtgtcc aggtggcatt ataaaatgta tatatggtgg atatagagag agagggagat
     2461 gcgtatagtg aataggaaag taagtaataa agagagggtg gaggaattgg aaaggggttg
     2521 gaggcaaacc cataaagagc attcatttcc ttttaaggtc gctgaaatta atgagtaacg
     2581 atcggtcaat gcctctcgct gacctttttc tttttttaca acaacaaata aaaataaaat
     2641 aaatttcgac gtctctttcc gctgctgaat tacatttgtt gaattaattt tctctgctta
     2701 cgtacgtctt ctaaactttc tctatccgaa ttctttttta actttctaac ttatattcaa
     2761 caactcttct ttcctgcctt taccgttagt ctaattgttt tcctaatact gctacgtaca
     2821 tacccctact atactagtca gtgtattaga ttcgattggg attaatccag gaatatagat
     2881 atcccattag tttttataaa aatattggaa gaggacaagt ctcaagcaat ttagggttcc
     2941 atgtagcgct gcaatatact gttagtaact ctctcttacc catatattgt atatgctaat
     3001 tcttatcaaa tatatatata tgcttctccc agagtcccag tttcctataa tcctgacgca
     3061 attatactaa tagagccaag tttacataat aaagtatata tgattaatag atagggtttc
     3121 ttattaagcc atatcttaaa ttaagatgtg atgatagcgt tttgtataag ttaccaattg
     3181 tttgaaagaa gagatcatca caataataaa tcataagtag tagtatatag taataaataa
     3241 atacacaagt cataataaga gtaatgagag gataattaag gagggaagaa gaaagcagaa
     3301 aatgcggttg gagaattagg tgctaaaagt tagttgagtc catctcagta tctaacggtc
     3361 aactctctct ctctctagag aaaacaatta agaaatctga catacacata tgtctctctc
     3421 tctctctctc tctagtctat acacacaatt caattaaaga agagacagag aagttcgtct
     3481 tttttgtttt tataccctta aatcaatcat gcaattgtaa cccttccttc ttattctcat
     3541 tccttccccc cctgtctaca gtaatctata gcaacgccat tatgtactac ttttaacgga
     3601 taatttgctc atgtttcaat atggcttcat tgtatatatg ttcaagttct tctcaatcct
     3661 ttatatcatt ccaacataat tcatattaaa gttagtagct gaaattggaa ggctgatata
     3721 ttttccataa ttcaaatttg aattttgctc atcatatata tatgtatata ttaaaaatcg
     3781 aatattaaga agaaaaatga agtcgatcga tggctgccaa tgctgtagct ggccatgttt
     3841 taaactactc aattgtcgga ttgaagtata gccaaaatat ataaaaccgt aaaaggacta
     3901 aatataataa tataataggt attaattaat taaaactaat taattataaa agaagcacct
     3961 aaaagtcaag agcagtagag aaatggaaga aatatctgaa aaacgaccgc ttatatatat
     4021 atgtatcatt ggaattgaag aggctatata tatatatata tatatatcga tcttagctta
     4081 tatattaatt gaaagtacat tttggtgtat aagtaattaa agaagaaaga aaaaaagaga
     4141 gataatatat aaggaagaag gagtgcgagg agaagaggga agagatcata attaagcaaa
     4201 gaagctagct agggacagga tttgtaacaa tgaaagcgtt aacggccagg caacaagagg
     4261 tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa
     4321 tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg
     4381 cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg
     4441 aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg tcgagcgccc
     4501 ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg
     4561 cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg
     4621 gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg
     4681 agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg gatccgtctg
     4741 ctggagacat gagagctgcc aacctttggc caagcccgct catgatcaaa cgctctaaga
     4801 agaacagcct ggccttgtcc ctgacggccg accagatggt cagtgccttg ttggatgctg
     4861 agccccccat actctattcc gagtatgatc ctaccagacc cttcagtgaa gcttcgatga
     4921 tgggcttact gaccaacctg gcagacaggg agctggttca catgatcaac tgggcgaaga
     4981 gggtgccagg ctttgtggat ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct
     5041 ggctagagat cctgatgatt ggacttgtct ggcgctccat ggagcaccca gggaagctac
     5101 tgtttgctcc taacttgctc ttggacagga accagggaaa atgtgtagag ggcatggtgg
     5161 agatcttcga catgctgctg gctacatcat ctcggttccg catgatgaat ctgcagggag
     5221 aggagtttgt gtgcctcaaa tctattattt tgcttaattc tggagtgtac acatttctgt
     5281 ccagcaccct gaagtctctg gaagagaagg accatatcca ccgagtcctg gacaagatca
     5341 cagacacttt gatccacctg atggccaagg caggcctgac cctgcagcag cagcaccagc
     5401 ggctggccca gctcctcctc atcctctccc acatcaggca catgagtaac aaaggcatgg
     5461 agcatctgta cagcatgaag tgcaagaacg tggtgcccct ctatgacctg ctgctggaga
     5521 tgctggacgc ccaccgccta catgcgccca ctagccgtgg aggggcatcc gtggaggaga
     5581 cggaccaaag ccacttggcc actgcgggct ctacttcatc gcattccttg caaaagtatt
     5641 acatcacggg ggaggcagag ggtttccctg ccacagtctg aggctgagct ccctggcgga
     5701 attcccagag atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa
     5761 tatcaaaaag cttctgtttc atcaaaaatg actcgaccta actgagtaag ctagcttgtt
     5821 cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta
     5881 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt
     5941 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta
     6001 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa
     6061 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa catcccaaac
     6121 tcagcctagc aaagtcgacg aattctcccg ggtggatcca aggccttcta gaaaaacgaa
     6181 ttccatgcca gcttgggctg caggtcgagg ctaaaaaact aatcgcatta tcatcccctc
     6241 gacgtactgt acatataacc actggtttta tatacagcag tactgtacat ataaccactg
     6301 gttttatata cagcagtcga cgtactgtac atataaccac tggttttata tacagcagta
     6361 ctgtacatat aaccactggt tttatataca gcagtcgagg taagattaga tatggatatg
     6421 tatatggata tgtatatggt ggtaatgcca tgtaatatgc tcgactctag gatcttcgca
     6481 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct gaagctagtc
     6541 gctgcatgga taagaagtac tctatcggac tcgatatcgg aactaactct gtgggatggg
     6601 ctgtgatcac cgatgagtac aaggtgccat ctaagaagtt caaggttctc ggaaacaccg
     6661 ataggcactc tatcaagaaa aaccttatcg gtgctctcct cttcgattct ggtgaaactg
     6721 ctgaggctac cagactcaag agaaccgcta gaagaaggta caccagaaga aagaacagga
     6781 tctgctacct ccaagagatc ttctctaacg agatggctaa agtggatgat tcattcttcc
     6841 acaggctcga agagtcattc ctcgtggaag aagataagaa gcacgagagg caccctatct
     6901 tcggaaacat cgttgatgag gtggcatacc acgagaagta ccctactatc taccacctca
     6961 gaaagaagct cgttgattct actgataagg ctgatctcag gctcatctac ctcgctctcg
     7021 ctcacatgat caagttcaga ggacacttcc tcatcgaggg tgatctcaac cctgataact
     7081 ctgatgtgga taagttgttc atccagctcg tgcagaccta caaccagctt ttcgaagaga
     7141 accctatcaa cgcttcaggt gtggatgcta aggctatcct ctctgctagg ctctctaagt
     7201 caagaaggct tgagaacctc attgctcagc tccctggtga gaagaagaac ggacttttcg
     7261 gaaacttgat cgctctctct ctcggactca cccctaactt caagtctaac ttcgatctcg
     7321 ctgaggatgc aaagctccag ctctcaaagg atacctacga tgatgatctc gataacctcc
     7381 tcgctcagat cggagatcag tacgctgatt tgttcctcgc tgctaagaac ctctctgatg
     7441 ctatcctcct cagtgatatc ctcagagtga acaccgagat caccaaggct ccactctcag
     7501 cttctatgat caagagatac gatgagcacc accaggatct cacacttctc aaggctcttg
     7561 ttagacagca gctcccagag aagtacaaag agattttctt cgatcagtct aagaacggat
     7621 acgctggtta catcgatggt ggtgcatctc aagaagagtt ctacaagttc atcaagccta
     7681 tcctcgagaa gatggatgga accgaggaac tcctcgtgaa gctcaataga gaggatcttc
     7741 tcagaaagca gaggaccttc gataacggat ctatccctca tcagatccac ctcggagagt
     7801 tgcacgctat ccttagaagg caagaggatt tctacccatt cctcaaggat aacagggaaa
     7861 agattgagaa gattctcacc ttcagaatcc cttactacgt gggacctctc gctagaggaa
     7921 actcaagatt cgcttggatg accagaaagt ctgaggaaac catcacccct tggaacttcg
     7981 aagaggtggt ggataagggt gctagtgctc agtctttcat cgagaggatg accaacttcg
     8041 ataagaacct tccaaacgag aaggtgctcc ctaagcactc tttgctctac gagtacttca
     8101 ccgtgtacaa cgagttgacc aaggttaagt acgtgaccga gggaatgagg aagcctgctt
     8161 ttttgtcagg tgagcaaaag aaggctatcg ttgatctctt gttcaagacc aacagaaagg
     8221 tgaccgtgaa gcagctcaaa gaggattact tcaagaaaat cgagtgcttc gattcagttg
     8281 agatttctgg tgttgaggat aggttcaacg catctctcgg aacctaccac gatctcctca
     8341 agatcattaa ggataaggat ttcttggata acgaggaaaa cgaggatatc ttggaggata
     8401 tcgttcttac cctcaccctc tttgaagata gagagatgat tgaagaaagg ctcaagacct
     8461 acgctcatct cttcgatgat aaggtgatga agcagttgaa gagaagaaga tacactggtt
     8521 ggggaaggct ctcaagaaag ctcattaacg gaatcaggga taagcagtct ggaaagacaa
     8581 tccttgattt cctcaagtct gatggattcg ctaacagaaa cttcatgcag ctcatccacg
     8641 atgattctct cacctttaaa gaggatatcc agaaggctca ggtttcagga cagggtgata
     8701 gtctccatga gcatatcgct aacctcgctg gatctcctgc aatcaagaag ggaatcctcc
     8761 agactgtgaa ggttgtggat gagttggtga aggtgatggg aaggcataag cctgagaaca
     8821 tcgtgatcga aatggctaga gagaaccaga ccactcagaa gggacagaag aactctaggg
     8881 aaaggatgaa gaggatcgag gaaggtatca aagagcttgg atctcagatc ctcaaagagc
     8941 accctgttga gaacactcag ctccagaatg agaagctcta cctctactac ctccagaacg
     9001 gaagggatat gtatgtggat caagagttgg atatcaacag gctctctgat tacgatgttg
     9061 atcatatcgt gccacagtca ttcttgaagg atgattctat cgataacaag gtgctcacca
     9121 ggtctgataa gaacaggggt aagagtgata acgtgccaag tgaagaggtt gtgaagaaaa
     9181 tgaagaacta ttggaggcag ctcctcaacg ctaagctcat cactcagaga aagttcgata
     9241 acttgactaa ggctgagagg ggaggactct ctgaattgga taaggcagga ttcatcaaga
     9301 ggcagcttgt ggaaaccagg cagatcacta agcacgttgc acagatcctc gattctagga
     9361 tgaacaccaa gtacgatgag aacgataagt tgatcaggga agtgaaggtt atcaccctca
     9421 agtcaaagct cgtgtctgat ttcagaaagg atttccaatt ctacaaggtg agggaaatca
     9481 acaactacca ccacgctcac gatgcttacc ttaacgctgt tgttggaacc gctctcatca
     9541 agaagtatcc taagctcgag tcagagttcg tgtacggtga ttacaaggtg tacgatgtga
     9601 ggaagatgat cgctaagtct gagcaagaga tcggaaaggc taccgctaag tatttcttct
     9661 actctaacat catgaatttc ttcaagaccg agattaccct cgctaacggt gagatcagaa
     9721 agaggccact catcgagaca aacggtgaaa caggtgagat cgtgtgggat aagggaaggg
     9781 atttcgctac cgttagaaag gtgctctcta tgccacaggt gaacatcgtt aagaaaaccg
     9841 aggtgcagac cggtggattc tctaaagagt ctatcctccc taagaggaac tctgataagc
     9901 tcattgctag gaagaaggat tgggacccta agaaatacgg tggtttcgat tctcctaccg
     9961 tggcttactc tgttctcgtt gtggctaagg ttgagaaggg aaagagtaag aagctcaagt
    10021 ctgttaagga acttctcgga atcactatca tggaaaggtc atctttcgag aagaacccaa
    10081 tcgatttcct cgaggctaag ggatacaaag aggttaagaa ggatctcatc atcaagctcc
    10141 caaagtactc actcttcgaa ctcgagaacg gtagaaagag gatgctcgct tctgctggtg
    10201 agcttcaaaa gggaaacgag cttgctctcc catctaagta cgttaacttt ctttacctcg
    10261 cttctcacta cgagaagttg aagggatctc cagaagataa cgagcagaag caacttttcg
    10321 ttgagcagca caagcactac ttggatgaga tcatcgagca gatctctgag ttctctaaaa
    10381 gggtgatcct cgctgatgca aacctcgata aggtgttgtc tgcttacaac aagcacagag
    10441 ataagcctat cagggaacag gcagagaaca tcatccatct cttcaccctt accaacctcg
    10501 gtgctcctgc tgctttcaag tacttcgata caaccatcga taggaagaga tacacctcta
    10561 ccaaagaagt gctcgatgct accctcatcc atcagtctat cactggactc tacgagacta
    10621 ggatcgatct ctcacagctc ggtggtgatt caagggctga tcctaagaag aagaggaagg
    10681 tttgagacgc aggcctccca gctttcgtcc gtatcatcgg tttcgacaac gttcgtcaag
    10741 ttcaatgcat cagtttcatt gcccacacac cagaatccta ctaagtttga gtattatggc
    10801 attggaaaag ctgttttctt ctatcatttg ttctgcttgt aatttactgt gttctttcag
    10861 tttttgtttt cggacatcaa aatgcaaatg gatggataag agttaataaa tgatatggtc
    10921 cttttgttca ttctcaaatt attattatct gttgttttta ctttaatggg ttgaatttaa
    10981 gtaagaaagg aactaacagt gtgatattaa ggtgcaatgt tagacatata aaacagtctt
    11041 tcacctctct ttggttatgt cttgaattgg tttgtttctt cacttatctg tgtaatcaag
    11101 tttactatga gtctatgatc aagtaattat gcaatcaagt taagtacagt ataggcttac
    11161 tattgaaacc aaattaacat agggttttta tttaaataaa agtttaacct tctttttaaa
    11221 aaattgttca tagtgtcatg tcagaacaag agctacaaat cacacatagc atgcataagc
    11281 ggagctatga tgagtggtat tgttttgttc gtcacttgtc actcttttcc aacacataat
    11341 cccgacaaca acgtaagagc atctctctct ctccacacac actcatgcat gcatgcattc
    11401 ttacacgtga ttgccatgca aatctccttt ctcacctata aatacaaacc aacccttcac
    11461 tacactcttc actcaaacca aaacaagaaa acatacacaa atagcaaaac aagggcgaat
    11521 tctcaacaca acatatacaa aacaaacgaa tctcaagcaa tcaagcattc tacttctatt
    11581 gcagcaattt aaatcatttc ttttaaagca aaagcaattt tctgaaaatt ttcaccattt
    11641 acgaacgata gccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
    11701 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
    11761 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
    11821 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
    11881 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
    11941 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
    12001 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
    12061 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
    12121 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
    12181 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
    12241 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
    12301 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
    12361 gctgtacaag tagcttggct gcaggtcgac ctgcaggcat gcccgctgaa atcaccagtc
    12421 tctctctaca aatctatctc tctctataat aatgtgtgag tagttcccag ataagggaat
    12481 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
    12541 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagggg
    12601 taccgtatga tctagggaag ttcctattcc gaagttccta ttctctgaaa agtataggaa
    12661 cttctttgcg tattgggcgc tcttggcctt tttggccacc ggtcgtacgg ttaaaaccac
    12721 cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
    12781 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
    12841 aatcaccact cgatacaggc agcccatcag tccactagag acgctcaccg ggctggttgc
    12901 cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa
    12961 gccgtgtgcg agacaccgca gccgccggcg ttgtggatac ctcgcggaaa acttggccct
    13021 cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt
    13081 gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat
    13141 cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat
    13201 aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc
    13261 cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg
    13321 gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg
    13381 ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg
    13441 ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct
    13501 cccggcccgc tctcgcgttg gcagcatcac ccataattgt ggtttcaaaa tcggctccgt
    13561 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
    13621 aaagttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagggaaggt
    13681 gcgaataagc ggggaaattc ttctcggctg actcagtcat ttcatttctt catgtttgag
    13741 ccgatttttt ctcccgtaaa tgccttgaat cagcctattt agaccgtttc ttcgccattt
    13801 aaggcgttat ccccagtttt tagtgagatc tctcccactg acgtatcatt tggtccgccc
    13861 gaaacaggtt ggccagcgtg aataacatcg ccagttggtt atcgtttttc agcaacccct
    13921 tgtatctggc tttcacgaag ccgaactgtc gcttgatgat gcgaaatggg tgctccaccc
    13981 tggcccggat gctggctttc atgtattcga tgttgatggc cgttttgttc ttgcgtggat
    14041 gctgtttcaa ggttcttacc ttgccggggc gctcggcgat cagccagtcc acatccacct
    14101 cggccagctc ctcgcgctgt ggcgcccctt ggtagccggc atcggctgag acaaattgct
    14161 cctctccatg cagcagatta cccagctgat tgaggtcatg ctcgttggcc gcggtggtga
    14221 ccaggctgtg ggtcaggcca ctcttggcat cgacaccaat gtgggccttc atgccaaagt
    14281 gccactgatt gcctttcttg gtctgatgca tctccggatc gcgttgctgc tctttgttct
    14341 tggtcgagct gggtgcctca atgatggtgg catcgaccaa ggtgccttga gtcatcatga
    14401 cgcctgcttc ggccagccag cgattgatgg tcttgaacaa ttggcgggcc agttgatgct
    14461 gctccagcag gtggcggaaa ttcatgatgg tggtgcggtc cggcaaggcg ctatccaggg
    14521 ataaccgggc aaacagacgc atggaggcga tttcgtacag agcatcttcc atcgcgccat
    14581 cgctcaggtt gtaccaatgc tgcatgcagt gaatgcgtag catggtttcc agcggataag
    14641 gtcgccggcc attaccagcc ttggggtaaa acggctcgat gacttccacc atgttttgcc
    14701 atggcagaat ctgctccatg cgggacaaga aaatctcttt tctggtctga cggcgcttac
    14761 tgctgaattc actgtcggcg aaggtaagtt gatgactcat gatgaaccct gttctatggc
    14821 tccagatgac aaacatgatc tcatatcagg gacttgttcg caccttcctt agcttcttgg
    14881 ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat
    14941 caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt
    15001 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
    15061 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
    15121 tggaaggaaa gctgcctgtt ccaaaggtcc tgcaccttga acggcatgat ggctggagca
    15181 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
    15241 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
    15301 tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac
    15361 tgaataacga tctggccgat gtggattgcg aaaactggga agaagacacc ccatttaaag
    15421 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
    15481 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
    15541 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
    15601 cgatcaggga ggatattggg gaagaacagt atgtcgagct attttttgac ttactgggga
    15661 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc
    15721 tagatgtggc gcaacgatgc tggcgacaag caggagcgca ccgacttctt ccgcatcaag
    15781 tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc
    15841 gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc
    15901 gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg atcaaatcag
    15961 gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat
    16021 cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag
    16081 gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc
    16141 gtcggctcga tggcccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct
    16201 ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag
    16261 gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag
    16321 cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca agccgcgttg
    16381 ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg
    16441 tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg
    16501 cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag
    16561 gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg
    16621 cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc
    16681 tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag
    16741 gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac
    16801 ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc
    16861 cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg
    16921 aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat
    16981 ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga
    17041 tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg
    17101 cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc
    17161 tagggccttg tggggtcagt tccggctggg ggttcagcag ccagcgcttt actgagatcc
    17221 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    17281 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    17341 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    17401 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    17461 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    17521 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    17581 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    17641 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    17701 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    17761 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    17821 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    17881 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    17941 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca a
//
