LOCUS       sequence-42626-e         463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Shin DH, Oganesyan N, Jancarik J, Yokota H, Kim R, Kim SH
  TITLE     Crystal structure of a nicotinate phosphoribosyltransferase from 
            Thermoplasma acidophilum.
  JOURNAL   J Biol Chem. 2005 May 6. 280(18):18326-35.
  PUBMED    15753098
REFERENCE   2  (bases 1 to 463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 May 6. 280(18):18326-35."
COMMENT     Sequence Label: pLIC.B4.Ta1145
FEATURES             Location/Qualifiers
     source          1..463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             71..93
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    108..132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(132..151)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(133..151)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(235..254)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gattaccagt ttaccttctt cagttttggt accgtgatga tgatgatgat tgctgctgcc
       61 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      121 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
      181 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      241 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      301 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      361 atgcaccatt ccttgcgggc ggcggtgctc aacggcctca acctactact ggggctgctt
      421 cctaatgcag gagtcgcata agggagagcg tcgagatccc gga
//
