LOCUS       sequence-426288-       13172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transient expression cassette for a Dexamethasone-inducible 
            E3-DART ligase, where the ligase is catalytically inactive..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: 
            pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-m
            cherry-Tnos
FEATURES             Location/Qualifiers
     source          1..13172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2012..2041
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3341..3367
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3873..3919
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             3936..3962
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4084..4095
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      6344..6596
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6631..6649)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6837..6861
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8162..8791
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9220..10293
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10359..10553
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10789..10811)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10897..11037
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10952..10971
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11052..11069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11223..11811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11303..11322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11899..12714)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11899..12714)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12622..12641
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    13147..13171
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaattc gtctcaggag agtctagctc aacagagctt ttaacccaaa ttggtacaat
       61 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
      121 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
      181 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
      241 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
      301 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
      361 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
      421 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
      481 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
      541 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
      601 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
      661 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
      721 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
      781 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
      841 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
      901 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
      961 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
     1021 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
     1081 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     1141 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     1201 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     1261 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     1321 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
     1381 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
     1441 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
     1501 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
     1561 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
     1621 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
     1681 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
     1741 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
     1801 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
     1861 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
     1921 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
     1981 ttaatctgag tttttctgat taacagccat ggagcagaaa ctcatctctg aagaggatct
     2041 tatgaagctt ctttcttcta tcgaacaagc atgcgatatt tgcagactta aaaagctcaa
     2101 gtgctctaaa gaaaaaccga agtgcgccaa gtgtcttaag aacaactggg agtgtagata
     2161 ctctcctaaa accaaaaggt ccccgcttac tagggcacat cttacagaag tggaatcaag
     2221 gcttgaaaga cttgaacagc tttttcttct tatttttcct aggtcttctg cccctccgac
     2281 cgatgtctct cttggggacg agctccacct tgacggagag gacgtggcta tggctcatgc
     2341 cgacgctctt gacgatttcg atcttgacat gttgggggac ggggattctc cgggtccggg
     2401 atttacccct cacgactctg ccccttacgg agctcttgat atggccgact tcgagtttga
     2461 gcagatgttt accgatgccc ttggaattga cgagtacggt ggggatccaa ttcagcaagc
     2521 cactgcagga gtctcacaag acacttctga aaatcctaac aaaacaatag ttcctgctgc
     2581 acttccacag ctcaccccta ccttggtgtc acttcttgag gtgattgaac ctgaggtgtt
     2641 gtatgcagga tatgattctt ctgttccaga ttcagcatgg agaattatga ccacactcaa
     2701 catgcttggt gggcgtcaag tgattgcagc agtgaaatgg gcaaaggcta taccaggatt
     2761 cagaaacctt caccttgatg accaaatgac ccttcttcag tactcatgga tgtttctcat
     2821 ggcatttgcc cttggttgga gatcatacag acaatcaagt ggaaaccttc tctgctttgc
     2881 tcctgatctt attattaatg agcagagaat gtctcttcct tgcatgtatg accaatgtaa
     2941 acacatgctt tttgtctctt ctgaacttca aagattgcag gtttcttatg aagagtatct
     3001 ctgtatgaaa acccttcttc ttctctcttc agttcctaag gaaggtctta agtctcaaga
     3061 gctttttgat gagattagaa tgacttatat caaagagctt ggaaaagcca tcgtcaaaag
     3121 ggaagggaac tctagtcaga actggcaaag attttaccaa cttacaaagc ttcttgactc
     3181 tatgcatgag gtggttgaga atctccttac ctactgcttc cagacatttt tggataagac
     3241 catgagtatt gaattcccag agatgcttgc tgaaatcatc actaatcaga taccaaaata
     3301 ttcaaatgga aatatcaaaa agcttctttt tcatcaaaaa tacccatacg atgttccaga
     3361 ttacgcttga ctcgacctaa ctgagtaagc tagcttgttc gagtattatg gcattgggaa
     3421 aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt
     3481 cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt
     3541 tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga
     3601 aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct
     3661 aatgaccgaa gttaatatga ggagtaaaac actagatccc caaacaagct tgaaactgaa
     3721 ggcgggaaac gacaatctga tctctagccc atcgggtgac agccctccga gcgggtgaca
     3781 gccctccgaa gcgggtgaca gccctccgac gtcgggtgac agccctccga gcgggtgaca
     3841 gccctccgag cgggtgacag ccctccgctg cagcaagacc cttcctctat ataaggaagt
     3901 tcatttcatt tggagaggac agcccaagct taatgtaccc atacgatgtt ccagattacg
     3961 ctgagtatga tgctgtctgg tcaaaatggg aaagggatgc accagcagga gagtcacctg
     4021 gaagagcagc agtggttcag gaaatgcgtg attgccttaa taacggaaat ccagtgctta
     4081 acgtgggagc ttcaggtctt accacccttc cagaccgtct tccaccgcat attacaacac
     4141 ttgttattcc agataataat ctcacctctt tgcctgagtt gccagaagga cttagagagc
     4201 ttgaggtgtc tggaaacctt cagcttacat ctcttccatc tcttccgcag ggtcttcaga
     4261 agttgtgggc ctataacaat tggcttgcct ctcttccgac tttgccgcca ggacttgggg
     4321 atcttgctgt ctctaataac cagcttacct ctcttccgga gatgccgcca gcacttagag
     4381 agcttagggt gtctggtaac aaccttacct ctcttccggc tcttccgtca ggacttcaga
     4441 agctttgggc atataacaat agattgacct ctttgccaga gatgtcacca ggtcttcagg
     4501 aacttgatgt ctctcataat cagcttacca gattgccgca atctctcact ggtctttctt
     4561 ctgctgcaag agtttatctt gacgggaatc cactttctgt tagaactctt caggctctta
     4621 gagacatcat tggacattca ggaatcagga tacacttcga tatggctggg ccttctgtcc
     4681 ctagagaagc cagagcactt caccttgctg tcgctgactg gcttacttct gcaagagagg
     4741 gggaagctgc ccaggcagac agatggcagg ctttcggact tgaagataac gccgccgcct
     4801 tctctcttgt ccttgacaga cttcgtgaga ctgaaaactt caaaaaagac gctggattta
     4861 aggcacagat atcatcttgg cttacacaac ttgctgaaga tgctgctctt agagcaaaaa
     4921 cctttgccat ggcaacagag gcaacatcaa ccgctgagga cagagtcaca catgcccttc
     4981 accagatgaa taacgttcaa cttgttcata atgcagaaaa aggggaatac gacaacaatc
     5041 tccaggggct tgtttctact gggcgtgaga tgttcagact tgcaacactt gaacagattg
     5101 ccagagaaaa agccggaaca cttgctcttg tcgatgacgt tgaggtctat cttgctttcc
     5161 agaataagct taaggaatca cttgagctta cctctgtgac ttcagaaatg cgtttctttg
     5221 acgtttctgg agtgactgtt tcagaccttc aggctgctga gcttcaggtg aaaaccgctg
     5281 aaaactctgg gttcagtaaa tggatacttc agtgggggcc gcttcactct gtgcttgaaa
     5341 gaaaagtgcc ggaaagattt aacgctcttc gtgaaaagca aatatctgat tatgaagaca
     5401 cttacagaaa gctttatgac gaagtgctta aatcttctgg gcttgtcgac gataccgatg
     5461 cagaacgtac tatcggagtt agtgctatgg atagtgctaa aaaagaattt cttgatggac
     5521 ttagagctct tgtggatgag gtgcttggtt cttatcttac agccagatgg cgtcttaact
     5581 tcggaggagg tggctcatct ggcggaggta tggtgagtaa gggtgaagaa gataatatgg
     5641 caataattaa agaatttatg cgttttaaag tccatatgga aggatcagtc aatggtcatg
     5701 aattcgaaat tgagggggaa ggtgaaggaa ggccgtatga ggggactcag accgctaaac
     5761 tcaaggtgac caagggtggg cctttgccgt ttgcatggga tatcctctct ccacaattta
     5821 tgtatggatc aaaggcttac gtcaagcacc cggctgacat acctgactat cttaaactct
     5881 ctttcccgga aggttttaag tgggaaagag tgatgaattt cgaagatggt ggggttgtga
     5941 ccgttacaca agattcaagt ctgcaggatg gagagtttat ttacaaggtt aaactcagag
     6001 gtaccaattt cccgtctgac gggcctgtga tgcaaaaaaa gactatgggg tgggaggcct
     6061 cttcagaaag aatgtatcct gaggatggag cactcaaggg tgaaattaaa caaagattga
     6121 agcttaaaga tggaggtcat tatgacgccg aagtgaaaac tacatataag gcaaagaagc
     6181 cagtccaatt gccgggagca tataatgtga atattaaact cgatatcacc tctcacaatg
     6241 aagattatac tatcgtcgaa cagtatgaaa gggccgaagg tcgtcattca accggaggta
     6301 tggatgagct ctataagtga gcttggaatg gatcttcgat cccgatcgtt caaacatttg
     6361 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt
     6421 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     6481 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     6541 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     6601 attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6661 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6781 aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcatgagac gaattctgac
     6841 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     6901 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     6961 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     7021 tgacgttcag tgcagccgtc atctgaaaac gacatgtcgc acaagtccta agttacgcga
     7081 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     7141 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     7201 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     7261 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     7321 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     7381 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     7441 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     7501 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     7561 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     7621 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     7681 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     7741 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     7801 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     7861 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     7921 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cctctcaacc gtgcggctgc
     7981 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     8041 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     8101 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     8161 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     8221 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     8281 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     8341 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     8401 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     8461 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     8521 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     8581 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     8641 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     8701 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     8761 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     8821 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     8881 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     8941 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     9001 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     9061 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     9121 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc
     9181 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     9241 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     9301 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     9361 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     9421 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     9481 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     9541 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     9601 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     9661 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     9721 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     9781 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     9841 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     9901 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     9961 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
    10021 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
    10081 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
    10141 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
    10201 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
    10261 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
    10321 gccctagcag gggaaaaagg tcgaaaagga ctctttcctg tggatagcac gtacattggg
    10381 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
    10441 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
    10501 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
    10561 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    10621 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
    10681 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
    10741 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10801 gcagctcccg gtgacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10861 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    10921 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    10981 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    11041 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    11101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    11161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    11221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    11281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11641 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    11701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11821 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11881 gtcatgcatt ctaggtgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
    11941 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
    12001 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
    12061 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
    12121 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
    12181 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
    12241 gttattcatt cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca
    12301 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
    12361 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt
    12421 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
    12481 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
    12541 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc ggtagattgt
    12601 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
    12661 gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt tgaatatggc tcataacaga
    12721 acttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
    12781 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
    12841 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
    12901 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
    12961 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
    13021 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
    13081 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
    13141 ggctggtggc aggatatatt gtggtgtaaa ca
//
