LOCUS       sequence-426297-        9688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human alpha-synuclein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Santhosh Kumar S, Naseri NN, Pather SR, Hallacli E, Ndayisaba A, 
            Buenaventura C, Acosta K, Roof J, Fazelinia H, Spruce LA, Luk K, 
            Khurana V, Rhoades E, Shalem O
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a 
            strategy to mitigate alpha-synuclein levels in human neurons.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj4767. doi: 10.1126/sciadv.adj4767. 
            Epub 2024 Feb 9.
  PUBMED    38335281
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj4767. doi"; pages: " 
            10.1126/sciadv.adj4767. Epub 2024 Feb 9"
COMMENT     Sequence Label: FUW-hSNCA-NE
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        215..594
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        596..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     749..769
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             812..992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1039..1164
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1657..1890
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2075..2119
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2599..3810
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3647..3666
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3839..3848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4357..4945
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4410..4430)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4828..4839)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4948..4964)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4949..4965)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5470..5650
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5676..5693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5682..5906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5952..6380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6039..6058)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6249..6270
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6389..6409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6394..6723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6574..6709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6636..6655
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6771..6818
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6837..7211
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7341..7462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7378..7397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7432..7451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7511..7527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7511..7527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7524..7546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7535..7551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7559..7565,7566..7583,7584..7589))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7604..7625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7742..7759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7913..8501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7993..8012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8672..9463,9464..9532))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9295..9314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9533..9637)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
       61 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
      121 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
      181 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      241 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      301 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      361 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      421 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      481 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      541 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      601 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      661 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      721 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      781 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      961 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     1021 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     1081 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     1141 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     1201 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     1261 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     1321 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1381 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1441 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1501 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1561 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1621 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1681 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1741 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1801 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1861 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1921 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1981 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     2041 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     2101 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     2161 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     2221 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     2281 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2341 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaacc
     2581 cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     2641 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     2701 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     2761 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     2821 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     2881 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     2941 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     3001 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     3061 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     3121 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     3181 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     3241 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     3301 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     3361 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     3421 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     3481 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     3541 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     3601 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     3661 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     3721 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     3781 ttctggccgt ttttggcttt tttgttagac gaagcttggg ctgcaggtcg actctagagc
     3841 caccatggat gtattcatga aaggactttc aaaggccaag gagggagttg tggctgctgc
     3901 tgagaaaacc aaacagggtg tggcagaagc agcaggaaag acaaaagagg gtgttctcta
     3961 tgtaggctcc aaaaccaagg agggagtggt gcatggtgtg gcaacagtgg ctgagaagac
     4021 caaagagcaa gtgacaaatg ttggaggagc agtggtgacg ggtgtgacag cagtagccca
     4081 gaagacagtg gagggagcag ggagcattgc agcagccact ggctttgtca aaaaggacca
     4141 gttgggcaag aatgaagaag gagccccaca ggaagggatt ctggaagata tgcctgtgga
     4201 tcctgacaat gaggcttatg aaatgccttc tgaggaaggg tatcaagact acgaacctga
     4261 agccaccaaa gaaaacccgc gtagcaacca ggaagaaagc tatgatgata acgaaagcta
     4321 aggatccgtt aacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     4381 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4441 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4501 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4561 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4621 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4681 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4741 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4801 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4861 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4921 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     4981 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     5041 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     5101 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5161 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     5221 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     5281 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     5341 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     5401 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     5461 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5521 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5581 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5641 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5701 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5761 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5821 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5881 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5941 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6001 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6061 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6121 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6181 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6241 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6301 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6361 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     6421 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     6481 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6541 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6601 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6661 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6721 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     6781 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     6841 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     6901 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     6961 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     7021 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     7081 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     7141 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     7201 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7261 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     7321 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     7381 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7441 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     7501 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8341 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8941 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9361 aactctcaag gatcttaccg ctgttgagat ccagttctat gtaacccact cgtgcaccca
     9421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9661 ctgacgtcga cggatcggga gatctccc
//
