LOCUS       sequence-426309-        9688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mutated alpha-synuclein (2nd amino acid 
            aspartate mutated to glutamate) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Santhosh Kumar S, Naseri NN, Pather SR, Hallacli E, Ndayisaba A, 
            Buenaventura C, Acosta K, Roof J, Fazelinia H, Spruce LA, Luk K, 
            Khurana V, Rhoades E, Shalem O
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a 
            strategy to mitigate alpha-synuclein levels in human neurons.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj4767. doi: 10.1126/sciadv.adj4767. 
            Epub 2024 Feb 9.
  PUBMED    38335281
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj4767. doi"; pages: " 
            10.1126/sciadv.adj4767. Epub 2024 Feb 9"
COMMENT     Sequence Label: FUW-hSNCA(D2E)-NE
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..45)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        217..596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        598..796
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     751..771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             814..994
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1041..1166
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1659..1892
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2077..2121
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2419..2536
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2601..3812
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3649..3668
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3841..3850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4359..4947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4412..4432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4830..4841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4950..4966)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4951..4967)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5472..5652
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5678..5695)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5684..5908
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5954..6382
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6041..6060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6251..6272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6391..6411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6396..6725
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6576..6711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6638..6657
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6773..6820
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6839..7213
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7343..7464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7380..7399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7434..7453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7513..7529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7513..7529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7526..7548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7537..7553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7561..7567,7568..7585,7586..7591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7606..7627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7744..7761)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7915..8503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7995..8014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8674..9465,9466..9534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9297..9316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9535..9639)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
       61 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      121 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      181 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      241 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      301 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      361 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      421 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      481 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      541 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      601 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      661 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      721 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      781 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      841 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      901 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      961 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     1021 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1081 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1141 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1201 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1261 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1321 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1381 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1441 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1501 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1561 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1621 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1681 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1741 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1801 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1861 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1921 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1981 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     2041 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2101 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2161 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2221 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2281 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2341 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     2401 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2461 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2521 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
     2581 cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc
     2641 cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc
     2701 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta
     2761 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc
     2821 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc
     2881 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
     2941 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt
     3001 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg
     3061 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga
     3121 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac
     3181 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     3241 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     3301 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg
     3361 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga
     3421 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg
     3481 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc
     3541 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga
     3601 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt
     3661 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg
     3721 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta
     3781 aattctggcc gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactctaga
     3841 gccaccatgg aggtattcat gaaaggactt tcaaaggcca aggagggagt tgtggctgct
     3901 gctgagaaaa ccaaacaggg tgtggcagaa gcagcaggaa agacaaaaga gggtgttctc
     3961 tatgtaggct ccaaaaccaa ggagggagtg gtgcatggtg tggcaacagt ggctgagaag
     4021 accaaagagc aagtgacaaa tgttggagga gcagtggtga cgggtgtgac agcagtagcc
     4081 cagaagacag tggagggagc agggagcatt gcagcagcca ctggctttgt caaaaaggac
     4141 cagttgggca agaatgaaga aggagcccca caggaaggga ttctggaaga tatgcctgtg
     4201 gatcctgaca atgaggctta tgaaatgcct tctgaggaag ggtatcaaga ctacgaacct
     4261 gaagccacca aagaaaaccc gcgtagcaac caggaagaaa gctatgatga taacgaaagc
     4321 taaggatccg ttaacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     4381 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4441 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4501 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4561 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4621 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4681 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4741 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     4801 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4861 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4921 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     4981 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     5041 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     5101 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5161 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     5221 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     5281 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     5341 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     5401 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     5461 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5641 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5701 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5761 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5821 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5881 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     5941 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6001 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6061 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6121 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6181 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6241 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6361 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6421 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6541 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6661 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6721 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     6781 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     6841 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     6901 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     6961 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     7021 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     7081 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     7141 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     7201 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     7261 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7321 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7441 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7501 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7561 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7621 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7681 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7741 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7801 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8101 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8341 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8641 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8701 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8761 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8821 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8881 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8941 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9001 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9061 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9361 aaaactctca aggatcttac cgctgttgag atccagttct atgtaaccca ctcgtgcacc
     9421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9661 acctgacgtc gacggatcgg gagatctc
//
