LOCUS       sequence-426311-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 Spike protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Qing E, Kicmal T, Kumar B, Hawkins GM, Timm E, Perlman S, Gallagher 
            T
  TITLE     Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control 
            Elements in the Amino-Terminal Domains.
  JOURNAL   mBio. 2021 Aug 31;12(4):e0159021. doi: 10.1128/mBio.01590-21. Epub 
            2021 Aug 3.
  PUBMED    34340537
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-08-31"; volume: "12(4):e0159021. doi"; pages: " 
            10.1128/mBio.01590-21. Epub 2021 Aug 3"
COMMENT     Sequence Label: pcDNA3.1-SARS-CoV-2-S (D614G)
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(26..817,818..886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     649..668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(887..991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1066..1085)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1257..1636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1637..1840
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1791..1811
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1885..1904
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1885..1903
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5848..5877
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5893..5910
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5933..5950)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5939..6163
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6209..6637
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6296..6315)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6506..6527
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6646..6666)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6651..6980
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6831..6966
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6893..6912
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7047..7841
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7101..7120)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7711..7730
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8017..8138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8054..8073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8108..8127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8187..8203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8187..8203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8200..8222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8211..8227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8235..8241,8242..8259,8260..8265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8280..8301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8418..8435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8589..9177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8669..8688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
       61 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
      121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
      181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
      241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
      301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
      361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
      421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
      481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
      541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
      601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
      661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
      721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
      781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
      841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
      901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     1021 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     1081 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     1141 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     1201 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     1261 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1321 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1381 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1441 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1501 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1561 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1621 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1681 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1741 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1801 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1861 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     1921 gttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc gagctcggta
     1981 cctcgcgaat gcatctagat gccaccatgt tcgtcttcct ggtcctgctg cctctggtct
     2041 cctcacagtg cgtcaatctg acaactcgga ctcagctgcc acctgcttat actaatagct
     2101 tcaccagagg cgtgtactat cctgacaagg tgtttagaag ctccgtgctg cactctacac
     2161 aggatctgtt tctgccattc tttagcaacg tgacctggtt ccacgccatc cacgtgagcg
     2221 gcaccaatgg cacaaagcgg ttcgacaatc ccgtgctgcc ttttaacgat ggcgtgtact
     2281 tcgcctctac cgagaagagc aacatcatca gaggctggat ctttggcacc acactggact
     2341 ccaagacaca gtctctgctg atcgtgaaca atgccaccaa cgtggtcatc aaggtgtgcg
     2401 agttccagtt ttgtaatgat cccttcctgg gcgtgtacta tcacaagaac aataagagct
     2461 ggatggagtc cgagtttaga gtgtattcta gcgccaacaa ctgcacattt gagtacgtga
     2521 gccagccttt cctgatggac ctggagggca agcagggcaa tttcaagaac ctgagggagt
     2581 tcgtgtttaa gaatatcgac ggctacttca aaatctactc taagcacacc cccatcaacc
     2641 tggtgcgcga cctgcctcag ggcttcagcg ccctggagcc cctggtggat ctgcctatcg
     2701 gcatcaacat cacccggttt cagacactgc tggccctgca cagaagctac ctgacacccg
     2761 gcgactcctc tagcggatgg accgccggcg ctgccgccta ctatgtgggc tacctccagc
     2821 cccggacctt cctgctgaag tacaacgaga atggcaccat cacagacgca gtggattgcg
     2881 ccctggaccc cctgagcgag acaaagtgta cactgaagtc ctttaccgtg gagaagggca
     2941 tctatcagac atccaatttc agggtgcagc caaccgagtc tatcgtgcgc tttcctaata
     3001 tcacaaacct gtgcccattt ggcgaggtgt tcaacgcaac ccgcttcgcc agcgtgtacg
     3061 cctggaatag gaagcggatc agcaactgcg tggccgacta tagcgtgctg tacaactccg
     3121 cctctttcag cacctttaag tgctatggcg tgtcccccac aaagctgaat gacctgtgct
     3181 ttaccaacgt ctacgccgat tctttcgtga tcaggggcga cgaggtgcgc cagatcgccc
     3241 ccggccagac aggcaagatc gcagactaca attataagct gccagacgat ttcaccggct
     3301 gcgtgatcgc ctggaacagc aacaatctgg attccaaagt gggcggcaac tacaattatc
     3361 tgtaccggct gtttagaaag agcaatctga agcccttcga gagggacatc tctacagaaa
     3421 tctaccaggc cggcagcacc ccttgcaatg gcgtggaggg ctttaactgt tatttcccac
     3481 tccagtccta cggcttccag cccacaaacg gcgtgggcta tcagccttac cgcgtggtgg
     3541 tgctgagctt tgagctgctg cacgccccag caacagtgtg cggccccaag aagtccacca
     3601 atctggtgaa gaacaagtgc gtgaacttca acttcaacgg cctgaccggc acaggcgtgc
     3661 tgaccgagtc caacaagaag ttcctgccat ttcagcagtt cggcagggac atcgcagata
     3721 ccacagacgc cgtgcgcgac ccacagaccc tggagatcct ggacatcaca ccctgctctt
     3781 tcggcggcgt gagcgtgatc acacccggca ccaatacaag caaccaggtg gccgtgctgt
     3841 atcagggcgt gaattgtacc gaggtgcccg tggctatcca cgccgatcag ctgaccccaa
     3901 catggcgggt gtacagcacc ggctccaacg tcttccagac aagagccgga tgcctgatcg
     3961 gagcagagca cgtgaacaat tcctatgagt gcgacatccc aatcggcgcc ggcatctgtg
     4021 cctcttacca gacccagaca aactctccca gaagagcccg gagcgtggcc tcccagtcta
     4081 tcatcgccta taccatgtcc ctgggcgccg agaacagcgt ggcctactct aacaatagca
     4141 tcgccatccc aaccaacttc acaatctctg tgaccacaga gatcctgccc gtgtccatga
     4201 ccaagacatc tgtggactgc acaatgtata tctgtggcga ttctaccgag tgcagcaacc
     4261 tgctgctcca gtacggcagc ttttgtaccc agctgaatag agccctgaca ggcatcgccg
     4321 tggagcagga taagaacaca caggaggtgt tcgcccaggt gaagcaaatc tacaagaccc
     4381 cccctatcaa ggactttggc ggcttcaatt tttcccagat cctgcctgat ccatccaagc
     4441 cttctaagcg gagctttatc gaggacctgc tgttcaacaa ggtgaccctg gccgatgccg
     4501 gcttcatcaa gcagtatggc gattgcctgg gcgacatcgc agccagggac ctgatctgcg
     4561 cccagaagtt taatggcctg accgtgctgc cacccctgct gacagatgag atgatcgcac
     4621 agtacacaag cgccctgctg gccggcacca tcacatccgg atggaccttc ggcgcaggag
     4681 ccgccctcca gatccccttt gccatgcaga tggcctatag gttcaacggc atcggcgtga
     4741 cccagaatgt gctgtacgag aaccagaagc tgatcgccaa tcagtttaac tccgccatcg
     4801 gcaagatcca ggacagcctg tcctctacag ccagcgccct gggcaagctc caggatgtgg
     4861 tgaatcagaa cgcccaggcc ctgaataccc tggtgaagca gctgagcagc aacttcggcg
     4921 ccatctctag cgtgctgaat gacatcctga gccggctgga caaggtggag gcagaggtgc
     4981 agatcgaccg gctgatcacc ggccggctcc agagcctcca gacctatgtg acacagcagc
     5041 tgatcagggc cgccgagatc agggccagcg ccaatctggc agcaaccaag atgtccgagt
     5101 gcgtgctggg ccagtctaag agagtggact tttgtggcaa gggctatcac ctgatgtcct
     5161 tccctcagtc tgccccacac ggcgtggtgt ttctgcacgt gacctacgtg cccgcccagg
     5221 agaagaactt caccacagcc cctgccatct gccacgatgg caaggcccac tttccaaggg
     5281 agggcgtgtt cgtgtccaac ggcacccact ggtttgtgac acagcgcaat ttctacgagc
     5341 cccagatcat caccacagac aacaccttcg tgagcggcaa ctgtgacgtg gtcatcggca
     5401 tcgtgaacaa taccgtgtat gatccactcc agcccgagct ggacagcttt aaggaggagc
     5461 tggataagta tttcaagaat cacacctccc ctgacgtgga tctgggcgac atcagcggca
     5521 tcaatgcctc cgtggtgaac atccagaagg agatcgaccg cctgaacgag gtggctaaga
     5581 atctgaacga gagcctgatc gacctccagg agctgggcaa gtatgagcag tacatcaagt
     5641 ggccctggta catctggctg ggcttcatcg ccggcctgat cgccatcgtg atggtgacca
     5701 tcatgctgtg ctgtatgaca tcctgctgtt cttgcctgaa gggctgctgt agctgtggct
     5761 cctgctgtaa gtttgacgag gatgactctg aacctgtgct gaagggcgtg aagctgcatt
     5821 acacctaaat cggatcccgg gcccttcgaa caaaaactca tctcagaaga ggatctgaat
     5881 atgcataccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     5941 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6001 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6061 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6121 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     6181 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     6241 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6301 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6361 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6421 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6481 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6541 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6601 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6661 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6721 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6781 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6841 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6901 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6961 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     7021 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     7081 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7141 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7201 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     7261 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     7321 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     7381 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     7441 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7501 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7561 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7621 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7681 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7741 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7801 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc
     7861 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7921 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7981 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8041 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8101 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8161 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8221 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8281 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8341 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8401 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8461 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9001 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9301 agttttaaat caatctaaag ta
//
