LOCUS       sequence-426316-       10369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression and easy visualization of alpha-synuclein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Santhosh Kumar S, Naseri NN, Pather SR, Hallacli E, Ndayisaba A, 
            Buenaventura C, Acosta K, Roof J, Fazelinia H, Spruce LA, Luk K, 
            Khurana V, Rhoades E, Shalem O
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a 
            strategy to mitigate alpha-synuclein levels in human neurons.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj4767. doi: 10.1126/sciadv.adj4767. 
            Epub 2024 Feb 9.
  PUBMED    38335281
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj4767. doi"; pages: " 
            10.1126/sciadv.adj4767. Epub 2024 Feb 9"
COMMENT     Sequence Label: FUW-hSNCA-GFP
FEATURES             Location/Qualifiers
     source          1..10369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..46)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        218..597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        599..797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     752..772
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             815..995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1042..1167
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1660..1893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2078..2122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2420..2537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3813
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3650..3669
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3842..3851
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4289..4291,4292..5005)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4289..4291,4292..5002)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4331..4352)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4592..4611)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4939..4960
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5041..5629
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5094..5114)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5512..5523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5632..5648)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5633..5649)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6154..6334
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6360..6377)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6366..6590
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6636..7064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6723..6742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6933..6954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7073..7093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7078..7407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7258..7393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7320..7339
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7455..7502
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7521..7895
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8025..8146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8062..8081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8116..8135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8195..8211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8195..8211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8208..8230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8219..8235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8243..8249,8250..8267,8268..8273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8288..8309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8426..8443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8597..9185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8677..8696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9356..10147,10148..10216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9979..9998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10217..10321)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
       61 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
      121 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
      181 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      241 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      301 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      361 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      421 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      481 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      541 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      601 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      661 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      721 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      781 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
      841 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      901 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      961 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1021 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1081 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1141 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1201 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1261 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1321 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1381 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1441 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1501 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1561 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1621 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1681 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1741 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1801 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1861 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1921 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1981 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2041 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2101 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2161 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2221 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2281 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2341 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     2401 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2461 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2521 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta
     2581 acccgtgtcg gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     2641 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     2701 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     2761 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     2821 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     2881 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     2941 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     3001 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     3061 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     3121 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     3181 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     3241 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     3301 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     3361 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     3421 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     3481 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     3541 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     3601 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     3661 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     3721 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     3781 aaattctggc cgtttttggc ttttttgtta gacgaagctt gggctgcagg tcgactctag
     3841 agccaccatg gatgtattca tgaaaggact ttcaaaggcc aaggagggag ttgtggctgc
     3901 tgctgagaaa accaaacagg gtgtggcaga agcagcagga aagacaaaag agggtgttct
     3961 ctatgtaggc tccaaaacca aggagggagt ggtgcatggt gtggcaacag tggctgagaa
     4021 gaccaaagag caagtgacaa atgttggagg agcagtggtg acgggtgtga cagcagtagc
     4081 ccagaagaca gtggagggag cagggagcat tgcagcagcc actggctttg tcaaaaagga
     4141 ccagttgggc aagaatgaag aaggagcccc acaggaaggg attctggaag atatgcctgt
     4201 ggatcctgac aatgaggctt atgaaatgcc ttctgaggaa gggtatcaag actacgaacc
     4261 tgaagccgct agcggtggcg gctctggtgt gagcaagggc gaggagctgt tcaccggggt
     4321 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     4381 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     4441 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     4501 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4561 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4621 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4681 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4741 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     4801 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4861 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     4921 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4981 cggcatggac gagctctaca agtgaggatc cgttaacaat tcgatatcaa gcttatcgat
     5041 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5101 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5161 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5221 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5281 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5341 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5401 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     5461 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5521 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5581 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     5641 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     5701 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     5761 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5821 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     5881 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     5941 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     6001 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     6061 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     6121 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     6181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6301 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     6361 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6421 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6481 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6541 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6601 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6781 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6841 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6901 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6961 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7021 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     7081 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7141 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7201 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgccgctaac tccgcccatc
     7261 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7321 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7381 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7441 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7501 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     7561 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     7621 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     7681 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     7741 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     7801 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     7861 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     7921 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7981 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     8041 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8101 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8161 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10081 ctatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10321 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatct
//
