LOCUS       sequence-426325-       10369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression and easy visualization of mutated 
            alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Santhosh Kumar S, Naseri NN, Pather SR, Hallacli E, Ndayisaba A, 
            Buenaventura C, Acosta K, Roof J, Fazelinia H, Spruce LA, Luk K, 
            Khurana V, Rhoades E, Shalem O
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a 
            strategy to mitigate alpha-synuclein levels in human neurons.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj4767. doi: 10.1126/sciadv.adj4767. 
            Epub 2024 Feb 9.
  PUBMED    38335281
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj4767. doi"; pages: " 
            10.1126/sciadv.adj4767. Epub 2024 Feb 9"
COMMENT     Sequence Label: FUW-hSNCA(D2E)-GFP
FEATURES             Location/Qualifiers
     source          1..10369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..42)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        214..593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        595..793
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     748..768
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             811..991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1038..1163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1656..1889
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2074..2118
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2416..2533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2598..3809
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3646..3665
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3838..3847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4285..4287,4288..5001)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4285..4287,4288..4998)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4327..4348)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4588..4607)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4935..4956
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5037..5625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5090..5110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5508..5519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5628..5644)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5629..5645)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6150..6330
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6356..6373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6362..6586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6632..7060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6719..6738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6929..6950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7069..7089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7074..7403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7254..7389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7316..7335
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7451..7498
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7517..7891
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8021..8142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8058..8077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8112..8131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8191..8207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8191..8207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8204..8226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8215..8231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8239..8245,8246..8263,8264..8269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8284..8305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8422..8439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8593..9181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8673..8692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9352..10143,10144..10212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9975..9994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10213..10317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
       61 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
      121 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      181 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      601 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      721 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      781 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      841 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      901 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      961 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     1021 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     1081 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1141 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1201 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1261 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1321 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1381 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1441 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1501 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1561 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1621 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1681 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1741 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1801 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1861 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1921 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1981 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     2041 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     2101 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     2161 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     2221 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2281 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2341 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     2401 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2461 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2521 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaaccc
     2581 gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
     2641 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
     2701 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
     2761 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
     2821 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
     2881 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
     2941 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
     3001 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
     3061 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
     3121 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
     3181 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
     3241 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     3301 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     3361 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     3421 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     3481 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     3541 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     3601 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     3661 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     3721 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     3781 tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga ctctagagcc
     3841 accatggagg tattcatgaa aggactttca aaggccaagg agggagttgt ggctgctgct
     3901 gagaaaacca aacagggtgt ggcagaagca gcaggaaaga caaaagaggg tgttctctat
     3961 gtaggctcca aaaccaagga gggagtggtg catggtgtgg caacagtggc tgagaagacc
     4021 aaagagcaag tgacaaatgt tggaggagca gtggtgacgg gtgtgacagc agtagcccag
     4081 aagacagtgg agggagcagg gagcattgca gcagccactg gctttgtcaa aaaggaccag
     4141 ttgggcaaga atgaagaagg agccccacag gaagggattc tggaagatat gcctgtggat
     4201 cctgacaatg aggcttatga aatgccttct gaggaagggt atcaagacta cgaacctgaa
     4261 gccgctagcg gtggcggctc tggtgtgagc aagggcgagg agctgttcac cggggtggtg
     4321 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4381 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4441 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4501 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4561 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4621 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4681 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4741 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4801 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4861 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4921 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4981 atggacgagc tctacaagtg aggatccgtt aacaattcga tatcaagctt atcgataatc
     5041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5401 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     5641 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     5701 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     5761 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5821 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     5881 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     5941 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     6001 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     6061 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     6121 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     6181 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6241 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6301 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     6361 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6421 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6481 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6541 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6601 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7081 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7321 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7381 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7441 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     7501 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     7561 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     7621 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     7681 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     7741 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     7801 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     7861 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     7921 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7981 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8161 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9001 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttctat
    10081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10321 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccg
//
