LOCUS       sequence-42633-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PP-ClpR
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(629..649)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(662..679)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             689..711
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    726..750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(750..769)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(751..769)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat cctcgagtgc ggccgcaagc ttcgtcatca ttccttttca agtatatgat
       61 caattaattt aaaatctact gcttcatctg cattaaaata tttatctctt tctaaaacat
      121 ttgaaataac atttgtatct ttttctgtat ttttagaaat tatttctatt actttttttt
      181 tggtgttcat tatttcttta ttttgtattt caatattggt tgcttggtta aagggtatga
      241 tagaataaga ttgatttaaa caaaaagagg aattttttaa agaaaagcga tagccttttt
      301 ttccactgct agctaatata caagcaattc catacgcttt tccaagacaa taagtataca
      361 catcagaaga tatataatta attacatcaa ctattgatat tacatcagtt ataccattca
      421 aatttattat tttattatta tctatatcac ctgtactgtt aatatataaa tgtataggtt
      481 ttcttttaga ttcatattct aaatataata actgtgatat gatttgttct gatatatgag
      541 gataaatagg tgatgataaa aatataattc ttttagataa aagtaatgaa ggtatattta
      601 tgttatactt gtgatgttca ttataggagc cctggaaata caagttttct ctgccgctgc
      661 tgtgatgatg atgatgatgc atggtatatc tccttcttaa agttaaacaa aattatttct
      721 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga
      781 tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      841 cggttgctgg cgcctatatc gccgacatc
//
