LOCUS       sequence-426336-        8193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CENP-T(1-242) transgene integration at AAVS1 safe harbor locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8193)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor
FEATURES             Location/Qualifiers
     source          1..8193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..409
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    111..129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    147..165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    219..237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    255..273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     318..342
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      416..425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(422..424,425..427,428..1138)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(467..488)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(728..747)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1075..1096
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1184..1204
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1217..1261
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(2310..2327)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2316..2540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2557..2678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2568..2587)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2622..2641
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2709..3308)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2792..2812)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3289..3308
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(3315..3368)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    3374..3399
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    3406..4209
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(4229..4817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4309..4328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4988..5779,5780..5848))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5611..5630
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5849..5953)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5990..6826
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    6834..6889
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(6918..7664)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             7687..7955
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(7968..8179)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gagagatctt ttcgtcttca acaattcttc gagtttactc cctatcagtg atagagaacg
       61 tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag
      121 tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta tatccagttt
      181 actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg
      241 tatatccagt ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg
      301 ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca
      361 acacttttgt cttataccaa ctttccgtac cacttcctac cctcgtaaag tcgacgccac
      421 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      481 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      541 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      601 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      661 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      721 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      781 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      841 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      901 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      961 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1021 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     1081 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     1141 cggaggggat cggtggagct ccaccggtgg cggccgctct agagagaatc tttattttca
     1201 gggcgccgcc aaattcaaag aaaccgctgc tgctaaattc gaacgccagc acatggacag
     1261 cggaggtgga ggtctcgaga tggcagacca caaccctgac agcgattcta cacctcgtac
     1321 actgctgcgt cgtgtgctgg atactgctga tcctcgtact cctcgtcgtc ctcgtagcgc
     1381 tcgtgctggt gctcgccgtg ccctgctgga aacagcctct cctcgtaaac tgagtggcca
     1441 gacccgtact attgcccgtg ggcgttcaca tggagcccgc tcggtcggtc gtagtgctca
     1501 cattcaagcc tcgggacacc tggaagaaca aacccctcgt accctgctga aaaacatcct
     1561 gctgactgcc cctgaaagct ctattctgat gccggaaagc gtcgttaaac cggttcctgc
     1621 tcctcaagcc gttcaacctt ctcgtcaaga gtcctcctgt ggatctctgg agctgcaact
     1681 gcctgaactg gagcctccta caacactggc accggggctg ctggcgcctg gtcgtcgtaa
     1741 acaacgtctg cgtctgagcg tgtttcaaca aggtgttgat cagggcctga gtctgtctca
     1801 agaacctcaa ggcaacgctg atgcttctag tctgacccgt tctctgaacc tgacctttgc
     1861 tactccactg caacctcaaa gcgtccaacg tcctggactg gctcgccgcc ctcctgctcg
     1921 ccgtgccgtt gatgtgggtg cttttctgcg tgacctgcgt gatacaagcc tggcacctcc
     1981 gaatattgtg ctggaggata cacagtgaca attccttaag tacgtaccgc gggctagcgg
     2041 atccatgcat acatgtgata tcactagtga ttttagggag agtttagtat ccctggttca
     2101 gaactccaga aaactacaga acattagaga caatgtggaa aaggaatcga aacgactgaa
     2161 aatatcttaa cccgggatcc accggatcta gataactgat cataatcagc cataccacat
     2221 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2281 aaatgaatgc aattaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2341 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2401 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2461 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     2521 ggatgcggtg ggctctatgg ggtaccacag acatgataag atacattgat gagtttggac
     2581 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2641 ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa gaactccagc
     2701 atacgcgttc aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc
     2761 tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg
     2821 gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg
     2881 acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac
     2941 cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc
     3001 agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct
     3061 tcgacgctct ccggcgtggt ccagaccgcc actgcggcgc cgtcgtccgc gacccacacc
     3121 ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg
     3181 atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
     3241 agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
     3301 tcggtcatat cgatagggcc gggattctcc tccacgtcac cgcatgttag aagacttcct
     3361 ctgccctctc cgccctgtgg gaggaagaga agaggtcagg cgcgtgcccc actgtggggt
     3421 ggaggggaca gataaaagta cccagaacca gagccacatt aaccggccct gggaatataa
     3481 ggtggtccca gctcggggac acaggatccc tggaggcagc aaacatgctg tcctgaagtg
     3541 gacatagggg cccgggttgg aggaagaaga ctagctgagc tctcggaccc ctggaagatg
     3601 ccatgacagg gggctggaag agctagcaca gactagagag gtaagggggg taggggagct
     3661 gcccaaatga aaggagtgag aggtgacccg aatccacagg agaacggggt gtccaggcaa
     3721 agaaagcaag aggatggaga ggtggctaaa gccagggaga cggggtactt tggggttgtc
     3781 cagaaaaacg gtgatgatgc aggcctacaa gaaggggagg cgggacgcaa gggagacatc
     3841 cgtcggagaa ggccatccta agaaacgaga gatggcacag gccccagaag gagaaggaaa
     3901 agggaaccca gcgagtgaag acggcatggg gttgggtgag ggaggagaga tgcccggaga
     3961 ggacccagac acggggagga tccgctcaga ggacatcacg tggtgcagcg ccgagaagga
     4021 agtgctccgg aaagagcatc cttgggcagc aacacagcag agagcaaggg gaagagggag
     4081 tggaggaaga cggaacctga aggaggcggc agggaaggat ctgggccagc cgtagaggtg
     4141 acccaggcca caagctgcag acagaaagcg gcacaggccc aggggagaga atgctggtca
     4201 gagaaagcag gcgcgccttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5101 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5881 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5941 taggggttcc gcgcacattt ccccgaaaag tgcttaagcc cttgccctta gagcagagcc
     6001 aggaacccct gtagggaagg ggcaggagag ccaggggcat gagatggtgg acgaggaagg
     6061 gggacaggga agcctgagcg cctctcctgg gcttgccaag gactcaaacc cagaagccca
     6121 gagcagggcc ttagggaagc gggaccctgc tctgggcgga ggaatatgtc ccagatagca
     6181 ctggggactc tttaaggaaa gaaggatgga gaaagagaaa gggagtagag gcggccacga
     6241 cctggtgaac acctaggacg caccattctc acaaagggag ttttccacac ggacaccccc
     6301 ctcctcacca cagccctgcc aggacggggc tggctactgg ccttatctca caggtaaaac
     6361 tgacgcacgg aggaacaata taaattgggg actagaaagg tgaagagcca aagttagaac
     6421 tcaggaccaa cttattctga ttttgttttt ccaaactgct tctcctcttg ggaagtgtaa
     6481 ggaagctgca gcaccaggat cagtgaaacg caccagacag ccgcgtcaga gcagctcagg
     6541 ttctgggaga gggtagcgca gggtggccac tgagaaccgg gcaggtcacg catccccccc
     6601 ttccctccca ccccctgcca agctctccct cccaggatcc tctctggctc catcgtaagc
     6661 aaaccttaga ggttctggca aggagagaga tggctccagg aaatgggggt gtgtcaccag
     6721 ataaggaatc tgcctaacag gaggtggggg ttagacccaa tatcaggaga ctaggaagga
     6781 ggaggcctaa ggatggggct tttctgtcac caatcctgtc cctagtaaga gcttgagaga
     6841 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttattc cggaataaag
     6901 gaaatttagt acccgggtta ccctgggagc atgtcaaggt caaaatcgtc aagagcgtca
     6961 gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg ctgggagcat gtctaagtca
     7021 aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact ttagctgttt ctccaggcca
     7081 catatgatta gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc
     7141 tcaattgctt gtttcagaag tgggggcata gaatcggtgg taggtgtctc tctttcctct
     7201 tttgctactt gatgctcctg ttcctccaat acgcagccca gtgtaaagtg gcccacggcg
     7261 gacagagcgt acagtgcgtt ctccagggag aagccttgct gacacaggaa cgcgagctga
     7321 ttttccaggg tttcgtactg tttctctgtt gggcgggtgc cgagatgcac tttagccccg
     7381 tcgcgatgtg agaggagagc acagcggtat gacttggcgt tgttccgcag aaagtcttgc
     7441 catgactcgc cttccagggg gcaggagtgg gtatgatgcc tgtccagcat ctcgattggc
     7501 agggcatcga gcagggcccg cttgttcttc acgtgccagt acagggtagg ctgctcaact
     7561 cccagctttt gagcgagttt ccttgtcgtc aggccttcga taccgactcc attgagtaat
     7621 tccagagcag agtttatgac tttgctcttg tccagtctag acatggtgct taattaacac
     7681 agcttggatc tggaacggcg cagaacagaa aacgaaacaa agacgtagag ttgagcaagc
     7741 agggtcaggc aaagcgtgga gagccggctg agtctaggta ggctccaagg gagcgccgga
     7801 caaaggcccg gtctcgacct gagctttaaa cttacctaga cggcggacgc agttcaggag
     7861 gcacctcagg cgggaggcgg cagaacgcga ctcaaccggc gtggatggcg gcctcaggta
     7921 gggcggcggg cgcgtgaagg agagatgcga gcccctcgaa gcttcagctg tgttctggcg
     7981 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     8041 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     8101 caccttctct aggcacccgt tcaattgccg acccctcccc ccaacttctc ggggactgtg
     8161 ggcgatgtgc gctctgccca ctgacgggca ccg
//
