LOCUS       sequence-426339-        8428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  I3-01 transgene integration at AAVS1 safe harbor locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8428)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS96_EGFP(A206K)-I3-01 AAVS donor
FEATURES             Location/Qualifiers
     source          1..8428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..410
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    76..94
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    112..130
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    148..166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    184..202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     319..343
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      417..426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(423..425,426..428,429..1139)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(468..489)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(729..748)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1076..1097
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1185..1205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1218..1262
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     intron          2035..2100
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(2546..2563)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2552..2776
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2793..2914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2804..2823)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2858..2877
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2945..3544)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3028..3048)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3525..3544
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(3551..3604)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    3610..3635
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    3642..4445
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(4465..5053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4545..4564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5224..6015,6016..6084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5847..5866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6085..6189)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6226..7062
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    7070..7125
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(7154..7900)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             7923..8191
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(8204..8415)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ggagagatct tttcgtcttc aacaattctt cgagtttact ccctatcagt gatagagaac
       61 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
      121 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atatccagtt
      181 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
      241 gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt
      301 gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac
      361 aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa gtcgacgcca
      421 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      481 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      541 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      601 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      661 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      721 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      781 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      841 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      901 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      961 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1021 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1081 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     1141 ccggagggga tcggtggagc tccaccggtg gcggccgctc tagagagaat ctttattttc
     1201 agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca
     1261 gcggaggtgg aggtctcgag atgaagatgg aggagctgtt caagaagcac aagatcgtgg
     1321 ccgtgctgag agccaacagc gtggaggagg ccaagaagaa ggccctggcc gtgttcctgg
     1381 gcggcgtgca cctgatcgag atcaccttca ccgtgcccga cgccgacacc gtgatcaagg
     1441 agctgagctt cctgaaggag atgggcgcca tcatcggcgc cggcaccgtg accagcgtgg
     1501 agcagtgcag aaaggccgtg gagagcggcg ccgagttcat cgtgagcccc cacctggacg
     1561 aggagatcag ccagttctgc aaggagaagg gcgtgttcta catgcccggc gtgatgaccc
     1621 ccaccgagct ggtgaaggcc atgaagctgg gccacaccat cctgaagctg ttccccggcg
     1681 aggtggtggg cccccagttc gtgaaggcca tgaagggccc cttccccaac gtgaagttcg
     1741 tgcccaccgg cggcgtgaac ctggacaacg tgtgcgagtg gttcaaggcc ggcgtgctgg
     1801 ccgtgggcgt gggcagcgcc ctggtgaagg gcacccccgt ggaggtggcc gagaaggcca
     1861 aggccttcgt ggagaagatc agaggctgca ccgagtgata gctcactcat taggcacccc
     1921 aggctttaca ctttatgctt ccgacctgca gccccttgga tctttgtgaa ggaaccttac
     1981 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     2041 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     2101 atcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa
     2161 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2221 tgaacctgaa acataaaatg aatgcaattc cttaagtacg taccgcgggc tagcggatcc
     2281 atgcatacat gtgatatcac tagtgatttt agggagagtt tagtatccct ggttcagaac
     2341 tccagaaaac tacagaacat tagagacaat gtggaaaagg aatcgaaacg actgaaaata
     2401 tcttaacccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     2461 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2521 gaatgcaatt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     2581 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2641 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2701 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2761 gcggtgggct ctatggggta ccacagacat gataagatac attgatgagt ttggacaaac
     2821 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2881 atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatac
     2941 gcgttcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
     3001 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
     3061 tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg
     3121 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
     3181 cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc
     3241 gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga
     3301 cgctctccgg cgtggtccag accgccactg cggcgccgtc gtccgcgacc cacaccttgc
     3361 cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt
     3421 ggcggtccgg atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg
     3481 tgcgtacggc cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg
     3541 tcatatcgat agggccggga ttctcctcca cgtcaccgca tgttagaaga cttcctctgc
     3601 cctctccgcc ctgtgggagg aagagaagag gtcaggcgcg tgccccactg tggggtggag
     3661 gggacagata aaagtaccca gaaccagagc cacattaacc ggccctggga atataaggtg
     3721 gtcccagctc ggggacacag gatccctgga ggcagcaaac atgctgtcct gaagtggaca
     3781 taggggcccg ggttggagga agaagactag ctgagctctc ggacccctgg aagatgccat
     3841 gacagggggc tggaagagct agcacagact agagaggtaa ggggggtagg ggagctgccc
     3901 aaatgaaagg agtgagaggt gacccgaatc cacaggagaa cggggtgtcc aggcaaagaa
     3961 agcaagagga tggagaggtg gctaaagcca gggagacggg gtactttggg gttgtccaga
     4021 aaaacggtga tgatgcaggc ctacaagaag gggaggcggg acgcaaggga gacatccgtc
     4081 ggagaaggcc atcctaagaa acgagagatg gcacaggccc cagaaggaga aggaaaaggg
     4141 aacccagcga gtgaagacgg catggggttg ggtgagggag gagagatgcc cggagaggac
     4201 ccagacacgg ggaggatccg ctcagaggac atcacgtggt gcagcgccga gaaggaagtg
     4261 ctccggaaag agcatccttg ggcagcaaca cagcagagag caaggggaag agggagtgga
     4321 ggaagacgga acctgaagga ggcggcaggg aaggatctgg gccagccgta gaggtgaccc
     4381 aggccacaag ctgcagacag aaagcggcac aggcccaggg gagagaatgc tggtcagaga
     4441 aagcaggcgc gccttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4981 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5101 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5161 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5221 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5281 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5341 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5401 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5461 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5521 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5581 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5641 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5701 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5761 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5821 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5881 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5941 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6001 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6061 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6121 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6181 ggttccgcgc acatttcccc gaaaagtgct taagcccttg cccttagagc agagccagga
     6241 acccctgtag ggaaggggca ggagagccag gggcatgaga tggtggacga ggaaggggga
     6301 cagggaagcc tgagcgcctc tcctgggctt gccaaggact caaacccaga agcccagagc
     6361 agggccttag ggaagcggga ccctgctctg ggcggaggaa tatgtcccag atagcactgg
     6421 ggactcttta aggaaagaag gatggagaaa gagaaaggga gtagaggcgg ccacgacctg
     6481 gtgaacacct aggacgcacc attctcacaa agggagtttt ccacacggac acccccctcc
     6541 tcaccacagc cctgccagga cggggctggc tactggcctt atctcacagg taaaactgac
     6601 gcacggagga acaatataaa ttggggacta gaaaggtgaa gagccaaagt tagaactcag
     6661 gaccaactta ttctgatttt gtttttccaa actgcttctc ctcttgggaa gtgtaaggaa
     6721 gctgcagcac caggatcagt gaaacgcacc agacagccgc gtcagagcag ctcaggttct
     6781 gggagagggt agcgcagggt ggccactgag aaccgggcag gtcacgcatc ccccccttcc
     6841 ctcccacccc ctgccaagct ctccctccca ggatcctctc tggctccatc gtaagcaaac
     6901 cttagaggtt ctggcaagga gagagatggc tccaggaaat gggggtgtgt caccagataa
     6961 ggaatctgcc taacaggagg tgggggttag acccaatatc aggagactag gaaggaggag
     7021 gcctaaggat ggggcttttc tgtcaccaat cctgtcccta gtaagagctt gagagacaca
     7081 aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattccgga ataaaggaaa
     7141 tttagtaccc gggttaccct gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
     7201 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
     7261 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
     7321 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
     7381 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
     7441 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
     7501 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
     7561 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc
     7621 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
     7681 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
     7741 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
     7801 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
     7861 gagcagagtt tatgactttg ctcttgtcca gtctagacat ggtgcttaat taacacagct
     7921 tggatctgga acggcgcaga acagaaaacg aaacaaagac gtagagttga gcaagcaggg
     7981 tcaggcaaag cgtggagagc cggctgagtc taggtaggct ccaagggagc gccggacaaa
     8041 ggcccggtct cgacctgagc tttaaactta cctagacggc ggacgcagtt caggaggcac
     8101 ctcaggcggg aggcggcaga acgcgactca accggcgtgg atggcggcct caggtagggc
     8161 ggcgggcgcg tgaaggagag atgcgagccc ctcgaagctt cagctgtgtt ctggcggcaa
     8221 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
     8281 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
     8341 ttctctaggc acccgttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg
     8401 atgtgcgctc tgcccactga cgggcacc
//
