LOCUS       sequence-426344-        4449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A11_STOP
FEATURES             Location/Qualifiers
     source          1..4449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2006..2105)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2122..2141)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2123..2141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2146..2162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2146..2162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2264..2283)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2275..3084
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2342..2361)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3231..3819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3720..3739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3973..3990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4149..4176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4268..4354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4403..4425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4417..4434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4418..4434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac cgcagagaag
      121 gccctgccac tgggaaatgg caaggcagca gaggaggcaa gagagagcga ggcccctgga
      181 ggaggatgca gctccggagg agcagcacct gcccggcacc caagagtgaa gagggataag
      241 gccgtgcacg agcgcggcca ctggaacaat aaggtggagt tcgtgctgtc cgtggcagga
      301 gagatcatcg gactgggcaa cgtgtggcgg ttcccctacc tgtgctataa gaacggcggc
      361 ggcgcctttc tgatcccata cgtggtgttc tttatctgct gtggcatccc cgtgttcttt
      421 ctggagaccg ccctgggaca gttcacaagc gagggaggca tcacctgctg gaggaaggtg
      481 tgccctctgt ttgagggcat cggctacgcc acccaggtca tcgaggccca cctgaacgtg
      541 tactatatca tcatcctggc ctgggccatc ttctatctgt ccaactgctt taccacagag
      601 ctgccatggg ccacatgcgg ccacgagtgg aataccgaga actgcgtgga gttccagaag
      661 ctgaacgtga gcaactactc tcacgtgagc ctgcagaatg ccacaagccc cgtgatggag
      721 ttttgggagc accgcgtgct ggccatctcc gatggcatcg agcacatcgg aaacctgaga
      781 tgggagctgg ccctgtgcct gctggccgcc tggaccatct gctacttctg tatctggaag
      841 ggcaccaagt ccacaggcaa ggtggtgtac gtgaccgcca catttcctta tatcatgctg
      901 ctgatcctgc tgatcagagg agtgacactg ccaggagcat ctgagggcat caagttctac
      961 ctgtatcccg acctgtccag gctgtctgat cctcaagtgt gggtggacgc cggcacacag
     1021 atcttctttt cctacgccat ctgcctggga tgtctgaccg ccctgggatc ttacaacaac
     1081 tacaacaaca actgctacag agactgtatc atgctgtgct gtctgaatag cggcacctcc
     1141 ttcgtggcag gattcgcaat cttttccgtg ctgggcttta tggcctacga gcagggagtg
     1201 ccaatcgcag aggtggcaga gtctggacca ggactggcct ttatcgccta tcccaaggcc
     1261 gtgacaatga tgccactgag ccccctgtgg gccaccctgt tctttatgat gctgatcttc
     1321 ctgggcctgg atagccagtt cgtgtgcgtg gagtccctgg tgacagccgt ggtggacatg
     1381 taccctaagg tgttccggag aggctatagg cgcgagctgc tgatcctggc cctgtctgtg
     1441 atcagctact ttctgggact ggtcatgctg accgagggag gaatgtacat cttccagctg
     1501 tttgactcct atgccgcctc tggcatgtgc ctgctgttcg tggccatctt tgagtgcatc
     1561 tgtatcggct gggtgtacgg ctctaataga ttctatgaca acatcgagga tatgatcggc
     1621 tacaggcccc ctagcctgat caagtggtgc tggatgatca tgacacccgg catctgtgcc
     1681 ggcatcttta tcttctttct gatcaagtac aagcccctga agtataataa catctacacc
     1741 tatcctgcct ggggctatgg catcggctgg ctgatggccc tgtctagcat gctgtgcatc
     1801 cctctgtgga tctgtatcac agtgtggaag accgagggca cactgcctga gaagctgcag
     1861 aagctgacca caccatctac cgatctgaag atgaggggca agctgggcgt gagcccacgg
     1921 atggtgacag tgaacgactg cgatgccaag ctgaagagcg acggcaccat cgccgccatc
     1981 accgagaagg agacacactt ctaagaccca gctttcttgt acaaagttgg cattataaga
     2041 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2101 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2161 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2221 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2281 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2341 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2401 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2461 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2521 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2581 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2641 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2701 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2761 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2821 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2881 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2941 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3001 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3061 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     3121 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     3181 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3361 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3721 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3781 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3841 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3901 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3961 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4021 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4081 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     4141 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4201 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     4261 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4321 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     4381 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4441 ctcgggccc
//
