LOCUS       sequence-426347-        4683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC44A4_STOP
FEATURES             Location/Qualifiers
     source          1..4683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2240..2339)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2356..2375)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2357..2375)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2380..2396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2380..2396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2498..2517)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2509..3318
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2576..2595)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3465..4053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3954..3973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4207..4224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4383..4410)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4502..4588)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4637..4659
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4651..4668
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4652..4668
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg cggcaagcag
      121 agggatgagg acgatgaggc ctacggcaag ccagtgaagt atgacccctc tttcagaggc
      181 cctatcaaga acaggagctg caccgatgtg atctgctgcg tgctgttcct gctgtttatc
      241 ctgggctaca tcgtggtggg catcgtggcc tggctgtacg gcgatccacg ccaggtgctg
      301 tatccccgga atagcacagg cgcctactgt ggcatgggcg agaacaagga caagccctac
      361 ctgctgtatt tcaatatctt ttcctgcatc ctgagctcca acatcatctc tgtggccgag
      421 aatggcctgc agtgtcctac cccacaggtg tgcgtgtcta gctgtcccga ggacccttgg
      481 acagtgggca agaacgagtt ttctcagacc gtgggcgagg tgttctacac aaagaacaga
      541 aatttttgcc tgccaggcgt gccctggaat atgaccgtga tcacaagcct gcagcaggag
      601 ctgtgcccta gcttcctgct gccttccgcc ccagccctgg gcaggtgttt tccatggacc
      661 aacgtgacac cacctgccct gccaggaatc accaatgata ccacaatcca gcagggcatc
      721 tctggcctga tcgacagcct gaacgcccgc gatatcagcg tgaagatctt cgaggacttt
      781 gcccagtcct ggtactggat cctggtggcc ctgggagtgg ccctggtgct gtccctgctg
      841 ttcatcctgc tgctgaggct ggtggcagga ccactggtgc tggtgctgat cctgggcgtg
      901 ctgggcgtgc tggcatatgg catctactat tgctgggagg agtaccgcgt gctgcgggat
      961 aagggcgcct ccatctctca gctgggcttc accacaaatc tgtctgccta tcagagcgtg
     1021 caggagacat ggctggccgc cctgatcgtg ctggccgtgc tggaggccat cctgctgctg
     1081 atgctgatct ttctgcggca gagaatcagg atcgccatcg ccctgctgaa ggaggccagc
     1141 aaggccgtgg gccagatgat gtccaccatg ttctaccccc tggtgacatt tgtgctgctg
     1201 ctgatctgta tcgcctactg ggccatgacc gccctgtatc tggccacatc tggccagcct
     1261 cagtatgtgc tgtgggccag caacatctcc tctcctggct gcgagaaggt gccaatcaac
     1321 acctcctgta atcctacagc ccacctggtg aatagctcct gcccaggcct gatgtgcgtg
     1381 ttccagggct attctagcaa gggcctgatc cagcggagcg tgttcaacct gcagatctac
     1441 ggcgtgctgg gcctgttttg gaccctgaat tgggtgctgg ccctgggaca gtgcgtgctg
     1501 gcaggagcct tcgccagctt ttattgggcc ttccacaagc cacaggatat ccccaccttc
     1561 cctctgatct ccgcctttat cagaaccctg aggtaccaca caggctctct ggcctttggc
     1621 gccctgatcc tgacactggt gcagatcgcc agagtgatcc tggagtatat cgaccacaag
     1681 ctgaggggag tgcagaaccc agtggcacgg tgcatcatgt gctgtttcaa gtgctgtctg
     1741 tggtgtctgg agaagttcat caagtttctg aacagaaatg cctacatcat gatcgccatc
     1801 tatggcaaga acttctgcgt gagcgccaag aatgccttta tgctgctgat gcgcaacatc
     1861 gtgcgggtgg tggtgctgga caaggtgacc gatctgctgc tgttctttgg caagctgctg
     1921 gtggtgggag gagtgggcgt gctgtccttc tttttctttt ctggcaggat ccctggcctg
     1981 ggcaaggatt tcaagagccc ccacctgaat tactattggc tgcctatcat gacctccatc
     2041 ctgggcgcct acgtgatcgc ctccggcttc ttcagcgtgt tcggcatgtg cgtggacaca
     2101 ctgttcctgt gcttcctgga ggatctggag agaaacaatg gcagcctgga caggccatac
     2161 tatatgagca agtccctgct gaagatcctg ggcaagaaga acgaggcccc acccgacaat
     2221 aagaagcgca agaagtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     2281 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     2341 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     2401 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2461 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2521 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2581 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2641 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2701 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2761 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2821 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2881 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2941 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     3001 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     3061 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     3121 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3181 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3241 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3301 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3361 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4261 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4321 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4381 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4441 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4501 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4561 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4621 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4681 ccc
//
