LOCUS       sequence-426349-       11499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Detection of peroxisome-lipid droplet contact site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Li X, Gamuyao R, Wu ML, Cho WJ, King SV, Petersen RA, Stabley DR, 
            Lindow C, Climer LK, Shirinifard A, Ferrara F, Throm RE, Robinson 
            CG, Zhou Y, Carisey AF, Tebo AG, Chang CL
  TITLE     A fluorogenic complementation tool kit for interrogating lipid 
            droplet-organelle interaction.
  JOURNAL   J Cell Biol. 2024 Sep 2;223(9):e202311126. doi: 
            10.1083/jcb.202311126. Epub 2024 Jul 1.
  PUBMED    38949658
REFERENCE   2  (bases 1 to 11499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-09-2"; volume: "223(9):e202311126. doi"; pages: 
            " 10.1083/jcb.202311126. Epub 2024 Jul 1"
COMMENT     Sequence Label: Lenti-FAB(PX-LD)
FEATURES             Location/Qualifiers
     source          1..11499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..38)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(22..38)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(35..57)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(70..76,77..94,95..100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        195..390
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      241..376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    396..530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(433..452)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     487..506
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(598..615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(769..1357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(849..868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1590..2381,2382..2450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2213..2232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        2589..2968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2969..3172
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             3187..3367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3414..3539
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4031..4148
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4508..4741
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4926..4970
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        5227..5530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5531..5734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5812..5821
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6748..6757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6802..7122
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             7153..7194
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          7671..7900
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7883..7902
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7972..8546,8547..8549,8550..8558)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8139..8156)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8366..8385
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      8890..8899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8896..9786
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     complement(11040..11059)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11105..11160
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11159..11178)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
       61 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      181 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      241 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      301 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      361 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
      421 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      481 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
      541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tggtggtaga
     1441 attggtaaag agagtcgtgt aaaatatcga gttcgcacat cttgttgtct gattattgat
     1501 ttttggcgaa accatttgat catatgacaa gatgtgtatc taccttaact taatgatttt
     1561 gataaaaatc attctagacg tctcgtgctt taccaatgct taatcagtga ggcacctatc
     1621 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1681 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1741 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1801 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1861 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1921 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1981 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2041 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2101 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2161 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2221 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2281 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2341 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2401 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat tgctggagac
     2461 gtctagaatt attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2521 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2581 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     2641 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2701 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     2761 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     2821 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     2881 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2941 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3001 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3061 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3121 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     3181 gaaccggggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3241 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3301 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3361 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     3421 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     3481 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     3541 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     3601 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     3661 ttaatactgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     3721 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     3781 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     3841 aagagcaaaa caaaagtaag aaaaaagcac agcaagcagc aggatcttca gacctggaaa
     3901 ttccctacaa tccccaaagt caaggagtag tagaatctat gaataaagaa ttaaagaaaa
     3961 ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat
     4021 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     4081 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     4141 aaaattttcg ggtttattac agggacagca gaaatccact ttggaaagga ccagcaaagc
     4201 tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc
     4261 caagaagaaa agcaaagatc attagggatt atggaaaaca gatggcaggt gatgattgtg
     4321 tggcaagtag acaggatgag gattagaaca tggaaaagtt tagtaaaaca ccataaggag
     4381 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     4441 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     4501 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     4561 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     4621 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     4681 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     4741 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     4801 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     4861 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     4921 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     4981 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     5041 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     5101 caccattatc gtttcagacc cacctcccaa ccccgagggg accgagctca agcttcgaac
     5161 gcgtgctagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     5281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     5341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     5401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     5461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     5581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     5641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     5701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg ctcgtttagt
     5761 gaaccctcga ggaattccgt acggaaccgg tcgatatctg cggcctagct agccaccatg
     5821 gcttccgtgc tgtcctacga aagcctggtc cacgccgtgg ccggagccgt gggaagcgtg
     5881 acagcaatga cagtgttttt tcccctggat acagctagac ttcgacttca ggttgatgag
     5941 aaaagaaaat ccaaaactac acacatggtg ctcctggaga tcattaaaga agaaggactc
     6001 ctggcaccat atcgagggtg gtttccagtg atttccagtc tctgctgctc caattttgtc
     6061 tatttctaca cttttaatag cctcaaagca ctctgggtca aaggtcaaca ttctaccact
     6121 ggaaaagatc tggtagttgg gtttgttgca ggagtggtta atgtgttgct aacaactcca
     6181 ctctgggtgg taaacaccag actgaagctt caaggagcaa aatttaggaa tgaagacatt
     6241 gtaccaacaa actacaaagg tatcattgat gcttttcatc agatcattcg cgatgaagga
     6301 atctcggctt tatggaatgg cacatttccc tcattgctgt tggtcttcaa tcctgccatc
     6361 cagttcatgt tttatgaagg tttaaaacgg cagcttttaa agaaacggat gaagctttct
     6421 tccttggatg tgttcatcat tggtgcagta gccaaagcga ttgccaccac ggtgacctat
     6481 cccctgcaga cggtacagtc aattctgagg tttgggcgtc atagactaaa cccagaaaac
     6541 agaacattgg gaagtcttcg gaatattctc tatcttcttc accaacgagt aagacgtttt
     6601 ggaataatgg gactctacaa aggccttgaa gccaaactgc tgcagacagt cctcactgct
     6661 gctctcatgt tccttgttta tgagaaactg acagctgcca ccttcacagt tatggggctg
     6721 aagcgtgcac accaacacct accggtcgcc accatggtga gcaagggcga ggagggtagc
     6781 ggtggaggtg gatcaggagg tggagttcag gtagaaacca tctccccagg agacgggcgc
     6841 accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga
     6901 aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag
     6961 gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa
     7021 ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca
     7081 catgccactc tcgtcttcga tgtggagctt ctaaaactgg aagggagtgg cagcggaggt
     7141 agtgggagcg gtggcaagcc catccccaac cccctgctgg gcctggacag caccaaactt
     7201 aatggcatgg acgagcggta caagtccgga ggaggcggca gcggcggagg gggatccatg
     7261 gagaccgtga gattcggcgg cgacgacatc gagaacagcc tggccaagat ggacgacaag
     7321 gccctggaca agctggcctt cggcgccatc cagctggacg gcaacggcaa gatcatccac
     7381 tacaacgccg ccgagggcac catcaccggc agagacccca agaccgtgat cggcaagaac
     7441 ttcttcaccg acgtggcccc cggcacccag agcaaggagt tccagggcag attcaaggag
     7501 ggcgtgcaga agggcgacct gaacaccatg ttcgagtgga tgatccccac cagcagaggc
     7561 cccaccaagg tgaaggtgca catgaagaag gccatgacct aagcggccgc atagataact
     7621 gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
     7681 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
     7741 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
     7801 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
     7861 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     7921 ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc ccccctctcc
     7981 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     8041 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     8101 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     8161 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     8221 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     8281 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     8341 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     8401 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     8461 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     8521 tttcctttga aaaacacgat gataagcttg ccacaacccg ggaggcgcgc catgggtgac
     8581 agctattggg tctttgtgaa acgggtgtcc actagtgaaa ccgaaaccgg aggcggctcc
     8641 ggaggcagcg gcggttctgg aggttccacc ctaccggtct cagctggagg cagtgctggt
     8701 ggaagtgccg gaggtagaat tctagaaggt gccgcaagag aggctgccgc tagggaagca
     8761 gctgcacgtg aggcagctgc tagagaagct gcagccaggg aggctgcagc tagagcatca
     8821 ggtggtggcg gttcaggcgg aggtggatct ggaggtggcg gatcgggtgg gggaggttcg
     8881 acaccggtcg ccaccatggc agaaatcggt actggctttc cattcgaccc ccattatgtg
     8941 gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg
     9001 ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt
     9061 gcaccgaccc atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca
     9121 gacctgggtt atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg
     9181 ggtctggaag aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg
     9241 gccaagcgca atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc
     9301 ccgacctggg acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc
     9361 gacgtcggcc gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg
     9421 ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat
     9481 cctgttgacc gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca
     9541 gcgaacatcg tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg
     9601 aagctgctgt tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg
     9661 gccaaaagcc tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa
     9721 gaagacaacc cggacctgat cggcagcgag atcgcgcgct ggctgtcgac gctcgagatt
     9781 tccggcatgt acaagtccgg actcagatct cgagctcaag catcgaattc cgaatccccg
     9841 cacaaacgca acctgtacta cttctcttac cccctttttg tcgggtttgc tctacttcga
     9901 ttggttgctt ttcatcttgg attactgttt gtttggttgt gtcaacgctt ttctagagcg
     9961 ctgatggctg cgaaacgttc ggctaatagt gaatcgcccc ataagaggaa cttatattac
    10021 ttcagctatc ccttgttcgt gggtttcgcg ctacttcggc tggtcgcgtt tcatctaggt
    10081 ttgttgtttg tctggttatg tcaacggttc tcgcgagcgt taatggcagc aaagaggtcg
    10141 gcaaatagcg aatcgcctca taaacgcaac ctctattact tctcttaccc gttgttcgtt
    10201 ggcttcgctc tccttcggct agttgcgttt catcttggtc ttctgttcgt gtggctttgc
    10261 caaagatttt cgcgagcttt aatggcggct aagaggagtg ccaatagcga atccccgcat
    10321 aagcggaact tatactactt ctcttaccct ctttttgtag gctttgctct actccggcta
    10381 gtggccttcc atctaggcct actattcgtc tggctctgcc agcgcttctc gcgagccttg
    10441 atggcagcta agcgttccgc aaatagcgaa tcacctcata agcggaatct gtactatttc
    10501 tcatatcctc tcttcgtagg gttcgcacta ttacgtcttg tagcatttca tctgggatta
    10561 ttattcgtct ggttgtgcca gcgattttca cgagccttaa tggctgcgaa gagaagcgca
    10621 aattctgaat ctcctcacaa gcggaacctc tattacttta gttacccgct cttcgtcggt
    10681 ttcgcgttac tcaggctggt tgcattccac ctcggcttgc tgttcgtatg gctttgccag
    10741 agattctccc gggcgctaat ggcagccaag cgctcctaag gatccaccgg atcggtacaa
    10801 ggcggccgca ctaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10861 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagatc
    10921 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    10981 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgtcgt gctagcagat
    11041 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
    11101 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
    11161 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
    11221 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
    11281 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
    11341 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
    11401 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
    11461 gctgtccctc ttctcttatg gagatccctc gacctgcag
//
