LOCUS       sequence-426353-       10665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Detection of ER-lipid droplet contact site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Li X, Gamuyao R, Wu ML, Cho WJ, King SV, Petersen RA, Stabley DR, 
            Lindow C, Climer LK, Shirinifard A, Ferrara F, Throm RE, Robinson 
            CG, Zhou Y, Carisey AF, Tebo AG, Chang CL
  TITLE     A fluorogenic complementation tool kit for interrogating lipid 
            droplet-organelle interaction.
  JOURNAL   J Cell Biol. 2024 Sep 2;223(9):e202311126. doi: 
            10.1083/jcb.202311126. Epub 2024 Jul 1.
  PUBMED    38949658
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-09-2"; volume: "223(9):e202311126. doi"; pages: 
            " 10.1083/jcb.202311126. Epub 2024 Jul 1"
COMMENT     Sequence Label: Lenti-FAB(ER-LD)
FEATURES             Location/Qualifiers
     source          1..10665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..79)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(63..79)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(76..98)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    87..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(111..117,118..135,136..141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    156..177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        236..431
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      282..417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    437..571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(474..493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     528..547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(639..656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(810..1398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(890..909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1631..2422,2423..2491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2254..2273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        2630..3009
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3010..3213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             3228..3408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3455..3580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4072..4189
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4549..4782
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4967..5011
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        5268..5571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5572..5775
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5853..5862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6243..6252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6591..6632
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          6878..7107
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7090..7109
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7179..7753,7754..7756,7757..7765)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7346..7363)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7573..7592
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      8097..8106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8103..8993
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     complement(10247..10266)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10312..10367
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10366..10385)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
       61 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
      241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
      301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      361 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
      421 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      481 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      541 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
      601 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1441 aacgaaaact cacgttaagg gattttggtc atggtggtag aattggtaaa gagagtcgtg
     1501 taaaatatcg agttcgcaca tcttgttgtc tgattattga tttttggcga aaccatttga
     1561 tcatatgaca agatgtgtat ctaccttaac ttaatgattt tgataaaaat cattctagac
     1621 gtctcgtgct ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2461 taagggcgac acggaaatgt tgaatactca ttgctggaga cgtctagaat tattattgaa
     2521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2581 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     2641 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     2701 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2761 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2821 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2881 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2941 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     3001 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     3061 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     3121 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     3181 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgggg tctctctggt
     3241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     3421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     3481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     3541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     3601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     3661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatactg gcctgttaga
     3721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     3781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     3841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     3901 gaaaaaagca cagcaagcag caggatcttc agacctggaa attccctaca atccccaaag
     3961 tcaaggagta gtagaatcta tgaataaaga attaaagaaa attataggac aggtaagaga
     4021 tcaggctgaa catcttaaga cagcagtaca aatggcagta ttcatccaca attttaaaag
     4081 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4141 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     4201 cagggacagc agaaatccac tttggaaagg accagcaaag ctcctctgga aaggtgaagg
     4261 ggcagtagta atacaagata atagtgacat aaaagtagtg ccaagaagaa aagcaaagat
     4321 cattagggat tatggaaaac agatggcagg tgatgattgt gtggcaagta gacaggatga
     4381 ggattagaac atggaaaagt ttagtaaaac accataagga ggagatatga gggacaattg
     4441 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     4501 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     4561 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     4621 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     4681 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     4741 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     4801 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     4861 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     4921 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     4981 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     5041 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     5101 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     5161 ccacctccca accccgaggg gaccgagctc aagcttcgaa cgcgtgctag ttattaatag
     5221 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     5281 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     5341 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     5401 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     5461 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     5521 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     5581 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     5641 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     5701 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     5761 tatataagca gagctcgttt agtgaaccgg gctcgtttag tgaaccctcg aggaattccg
     5821 tacggaaccg gtcgatatct gcggcctagc tagccaccat ggagaccgtg agattcggcg
     5881 gcgacgacat cgagaacagc ctggccaaga tggacgacaa ggccctggac aagctggcct
     5941 tcggcgccat ccagctggac ggcaacggca agatcatcca ctacaacgcc gccgagggca
     6001 ccatcaccgg cagagacccc aagaccgtga tcggcaagaa cttcttcacc gacgtggccc
     6061 ccggcaccca gagcaaggag ttccagggca gattcaagga gggcgtgcag aagggcgacc
     6121 tgaacaccat gttcgagtgg atgatcccca ccagcagagg ccccaccaag gtgaaggtgc
     6181 acatgaagaa ggccatgacc tccggaggag gcggcagcgg cggaggggga tctctaccgg
     6241 tcgccaccat ggtgagcaag ggcgaggagg gcagcggcgg agggggatca ggagggggcg
     6301 agatgtggca tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa aggaacgtga
     6361 aaggcatgtt tgaggtgctg gagcccttgc atgctatgat ggaacggggc ccccagactc
     6421 tgaaggaaac atcctttaat caggcctatg gtcgagattt aatggaggcc caagagtggt
     6481 gcaggaagta catgaaatca gggaatgtca aggacctcac ccaagcctgg gacctctatt
     6541 atcatgtgtt ccgacgaatc tcaagtggca gcggaggtag tgggagcggt ggcaagccca
     6601 tccccaaccc cctgctgggc ctggacagca ccaaacttaa tggcatggac gagcggtaca
     6661 agtccggact cagatctcga gctcaagctt cgaattcctc gatcaccacc gtggagtcca
     6721 actcctcctg gtggaccaac tgggtgatcc ccgccatctc cgccctggtg gtggccctga
     6781 tgtaccgcct gtacatggcc gaggactaag cggccgcata gataactgat ccagtgtgct
     6841 ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg
     6901 ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc
     6961 tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt
     7021 ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg
     7081 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc
     7141 atgcatctag ggcggccaat tccgcccctc tccccccccc cctctccctc ccccccccct
     7201 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     7261 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     7321 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     7381 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     7441 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     7501 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     7561 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     7621 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     7681 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     7741 acacgatgat aagcttgcca caacccggga ggcgcgccat gggtgacagc tattgggtct
     7801 ttgtgaaacg ggtgtccact agtgaaaccg aaaccggagg cggctccgga ggcagcggcg
     7861 gttctggagg ttccacccta ccggtctcag ctggaggcag tgctggtgga agtgccggag
     7921 gtagaattct agaaggtgcc gcaagagagg ctgccgctag ggaagcagct gcacgtgagg
     7981 cagctgctag agaagctgca gccagggagg ctgcagctag agcatcaggt ggtggcggtt
     8041 caggcggagg tggatctgga ggtggcggat cgggtggggg aggttcgaca ccggtcgcca
     8101 ccatggcaga aatcggtact ggctttccat tcgaccccca ttatgtggaa gtcctgggcg
     8161 agcgcatgca ctacgtcgat gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg
     8221 gtaacccgac ctcctcctac gtgtggcgca acatcatccc gcatgttgca ccgacccatc
     8281 gctgcattgc tccagacctg atcggtatgg gcaaatccga caaaccagac ctgggttatt
     8341 tcttcgacga ccacgtccgc ttcatggatg ccttcatcga agccctgggt ctggaagagg
     8401 tcgtcctggt cattcacgac tggggctccg ctctgggttt ccactgggcc aagcgcaatc
     8461 cagagcgcgt caaaggtatt gcatttatgg agttcatccg ccctatcccg acctgggacg
     8521 aatggccaga atttgcccgc gagaccttcc aggccttccg caccaccgac gtcggccgca
     8581 agctgatcat cgatcagaac gtttttatcg agggtacgct gccgatgggt gtcgtccgcc
     8641 cgctgactga agtcgagatg gaccattacc gcgagccgtt cctgaatcct gttgaccgcg
     8701 agccactgtg gcgcttccca aacgagctgc caatcgccgg tgagccagcg aacatcgtcg
     8761 cgctggtcga agaatacatg gactggctgc accagtcccc tgtcccgaag ctgctgttct
     8821 ggggcacccc aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc
     8881 ctaactgcaa ggctgtggac atcggcccgg gtctgaatct gctgcaagaa gacaacccgg
     8941 acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct cgagatttcc ggcatgtaca
     9001 agtccggact cagatctcga gctcaagcat cgaattccga atccccgcac aaacgcaacc
     9061 tgtactactt ctcttacccc ctttttgtcg ggtttgctct acttcgattg gttgcttttc
     9121 atcttggatt actgtttgtt tggttgtgtc aacgcttttc tagagcgctg atggctgcga
     9181 aacgttcggc taatagtgaa tcgccccata agaggaactt atattacttc agctatccct
     9241 tgttcgtggg tttcgcgcta cttcggctgg tcgcgtttca tctaggtttg ttgtttgtct
     9301 ggttatgtca acggttctcg cgagcgttaa tggcagcaaa gaggtcggca aatagcgaat
     9361 cgcctcataa acgcaacctc tattacttct cttacccgtt gttcgttggc ttcgctctcc
     9421 ttcggctagt tgcgtttcat cttggtcttc tgttcgtgtg gctttgccaa agattttcgc
     9481 gagctttaat ggcggctaag aggagtgcca atagcgaatc cccgcataag cggaacttat
     9541 actacttctc ttaccctctt tttgtaggct ttgctctact ccggctagtg gccttccatc
     9601 taggcctact attcgtctgg ctctgccagc gcttctcgcg agccttgatg gcagctaagc
     9661 gttccgcaaa tagcgaatca cctcataagc ggaatctgta ctatttctca tatcctctct
     9721 tcgtagggtt cgcactatta cgtcttgtag catttcatct gggattatta ttcgtctggt
     9781 tgtgccagcg attttcacga gccttaatgg ctgcgaagag aagcgcaaat tctgaatctc
     9841 ctcacaagcg gaacctctat tactttagtt acccgctctt cgtcggtttc gcgttactca
     9901 ggctggttgc attccacctc ggcttgctgt tcgtatggct ttgccagaga ttctcccggg
     9961 cgctaatggc agccaagcgc tcctaaggat ccaccggatc ggtacaaggc ggccgcacta
    10021 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    10081 aaagggggga ctggaagggc taattcactc ccaaagaaga caagatctgc tttttgcctg
    10141 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10201 cccactgctt aagcctcaat aaagcttgcc ttgtcgtgct agcagatctt tttccctctg
    10261 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
    10321 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
    10381 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
    10441 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
    10501 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
    10561 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
    10621 ttttactagc cagatttttc ctcctctcct gactactccc agtca
//
