LOCUS       sequence-426356-       10432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Detection of mito-lipid droplet contact site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Li X, Gamuyao R, Wu ML, Cho WJ, King SV, Petersen RA, Stabley DR, 
            Lindow C, Climer LK, Shirinifard A, Ferrara F, Throm RE, Robinson 
            CG, Zhou Y, Carisey AF, Tebo AG, Chang CL
  TITLE     A fluorogenic complementation tool kit for interrogating lipid 
            droplet-organelle interaction.
  JOURNAL   J Cell Biol. 2024 Sep 2;223(9):e202311126. doi: 
            10.1083/jcb.202311126. Epub 2024 Jul 1.
  PUBMED    38949658
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-09-2"; volume: "223(9):e202311126. doi"; pages: 
            " 10.1083/jcb.202311126. Epub 2024 Jul 1"
COMMENT     Sequence Label: Lenti-FAB(mito-LD)
FEATURES             Location/Qualifiers
     source          1..10432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2027..2046)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2092..2147
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2146..2165)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2508..2524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2508..2524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2521..2543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2532..2548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2556..2562,2563..2580,2581..2586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2601..2622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2681..2876
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2727..2862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2882..3016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2919..2938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2973..2992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3084..3101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3255..3843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3335..3354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4076..4867,4868..4936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4699..4718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        5075..5454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5455..5658
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5673..5853
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5900..6025
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6517..6634
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6994..7227
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7412..7456
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        7713..8016
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8017..8220
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8298..8307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8697..8777
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             8796..8837
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          9089..9318
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     9301..9320
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(9390..9965,9966..9968,9969..9977)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9558..9575)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9785..9804
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      10309..10318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10315..10432,1..773)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 gtaacccgac ctcctcctac gtgtggcgca acatcatccc gcatgttgca ccgacccatc
       61 gctgcattgc tccagacctg atcggtatgg gcaaatccga caaaccagac ctgggttatt
      121 tcttcgacga ccacgtccgc ttcatggatg ccttcatcga agccctgggt ctggaagagg
      181 tcgtcctggt cattcacgac tggggctccg ctctgggttt ccactgggcc aagcgcaatc
      241 cagagcgcgt caaaggtatt gcatttatgg agttcatccg ccctatcccg acctgggacg
      301 aatggccaga atttgcccgc gagaccttcc aggccttccg caccaccgac gtcggccgca
      361 agctgatcat cgatcagaac gtttttatcg agggtacgct gccgatgggt gtcgtccgcc
      421 cgctgactga agtcgagatg gaccattacc gcgagccgtt cctgaatcct gttgaccgcg
      481 agccactgtg gcgcttccca aacgagctgc caatcgccgg tgagccagcg aacatcgtcg
      541 cgctggtcga agaatacatg gactggctgc accagtcccc tgtcccgaag ctgctgttct
      601 ggggcacccc aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc
      661 ctaactgcaa ggctgtggac atcggcccgg gtctgaatct gctgcaagaa gacaacccgg
      721 acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct cgagatttcc ggcatgtaca
      781 agtccggact cagatctcga gctcaagcat cgaattccga atccccgcac aaacgcaacc
      841 tgtactactt ctcttacccc ctttttgtcg ggtttgctct acttcgattg gttgcttttc
      901 atcttggatt actgtttgtt tggttgtgtc aacgcttttc tagagcgctg atggctgcga
      961 aacgttcggc taatagtgaa tcgccccata agaggaactt atattacttc agctatccct
     1021 tgttcgtggg tttcgcgcta cttcggctgg tcgcgtttca tctaggtttg ttgtttgtct
     1081 ggttatgtca acggttctcg cgagcgttaa tggcagcaaa gaggtcggca aatagcgaat
     1141 cgcctcataa acgcaacctc tattacttct cttacccgtt gttcgttggc ttcgctctcc
     1201 ttcggctagt tgcgtttcat cttggtcttc tgttcgtgtg gctttgccaa agattttcgc
     1261 gagctttaat ggcggctaag aggagtgcca atagcgaatc cccgcataag cggaacttat
     1321 actacttctc ttaccctctt tttgtaggct ttgctctact ccggctagtg gccttccatc
     1381 taggcctact attcgtctgg ctctgccagc gcttctcgcg agccttgatg gcagctaagc
     1441 gttccgcaaa tagcgaatca cctcataagc ggaatctgta ctatttctca tatcctctct
     1501 tcgtagggtt cgcactatta cgtcttgtag catttcatct gggattatta ttcgtctggt
     1561 tgtgccagcg attttcacga gccttaatgg ctgcgaagag aagcgcaaat tctgaatctc
     1621 ctcacaagcg gaacctctat tactttagtt acccgctctt cgtcggtttc gcgttactca
     1681 ggctggttgc attccacctc ggcttgctgt tcgtatggct ttgccagaga ttctcccggg
     1741 cgctaatggc agccaagcgc tcctaaggat ccaccggatc ggtacaaggc ggccgcacta
     1801 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     1861 aaagggggga ctggaagggc taattcactc ccaaagaaga caagatctgc tttttgcctg
     1921 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     1981 cccactgctt aagcctcaat aaagcttgcc ttgtcgtgct agcagatctt tttccctctg
     2041 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     2101 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     2161 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     2221 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     2281 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     2341 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     2401 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     2461 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
     2521 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     2581 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     2641 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
     2701 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2761 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     2821 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     2881 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2941 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3001 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3901 taagggattt tggtcatggt ggtagaattg gtaaagagag tcgtgtaaaa tatcgagttc
     3961 gcacatcttg ttgtctgatt attgattttt ggcgaaacca tttgatcata tgacaagatg
     4021 tgtatctacc ttaacttaat gattttgata aaaatcattc tagacgtctc gtgctttacc
     4081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4921 aatgttgaat actcattgct ggagacgtct agaattatta ttgaagcatt tatcagggtt
     4981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5041 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
     5101 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     5161 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     5221 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     5281 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     5341 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     5401 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     5461 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     5521 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     5581 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     5641 gtctatataa gcagagctcg tttagtgaac cggggtctct ctggttagac cagatctgag
     5701 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5761 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5821 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     5881 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     5941 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     6001 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     6061 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     6121 aagcagggag ctagaacgat tcgcagttaa tactggcctg ttagaaacat cagaaggctg
     6181 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     6241 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     6301 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagaaaa aagcacagca
     6361 agcagcagga tcttcagacc tggaaattcc ctacaatccc caaagtcaag gagtagtaga
     6421 atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg ctgaacatct
     6481 taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     6541 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     6601 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaaa
     6661 tccactttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag tagtaataca
     6721 agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatta gggattatgg
     6781 aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt agaacatgga
     6841 aaagtttagt aaaacaccat aaggaggaga tatgagggac aattggagaa gtgaattata
     6901 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     6961 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7021 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7081 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7141 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7201 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7261 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7321 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7381 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7441 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     7501 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     7561 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     7621 gaggggaccg agctcaagct tcgaacgcgt gctagttatt aatagtaatc aattacgggg
     7681 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7741 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7801 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7861 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7921 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     7981 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     8041 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     8101 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     8161 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     8221 cgtttagtga accgggctcg tttagtgaac cctcgaggaa ttccgtacgg aaccggtcga
     8281 tatctgcggc ctagctagcc accatggaga ccgtgagatt cggcggcgac gacatcgaga
     8341 acagcctggc caagatggac gacaaggccc tggacaagct ggccttcggc gccatccagc
     8401 tggacggcaa cggcaagatc atccactaca acgccgccga gggcaccatc accggcagag
     8461 accccaagac cgtgatcggc aagaacttct tcaccgacgt ggcccccggc acccagagca
     8521 aggagttcca gggcagattc aaggagggcg tgcagaaggg cgacctgaac accatgttcg
     8581 agtggatgat ccccaccagc agaggcccca ccaaggtgaa ggtgcacatg aagaaggcca
     8641 tgacctccgg aggaggcggc agcggcggag ggggatcacc ggtcgccggt agcggttacc
     8701 catacgatgt tccagattac gcttatcctt atgacgtacc tgactatgca tacccttatg
     8761 atgtaccaga ctacgcttca ggaggtggag ggagtggcaa gcccatcccc aaccccctgc
     8821 tgggcctgga cagcaccaaa cttggtagtg ggagcggctc cggagcttcg aattctttgc
     8881 aggtgcagaa tggacctata ggacatcgag gtgaagggga cccaagtggt attcccatat
     8941 ttatggtgct ggtgccagtg tttgccctca ccatggtagc agcctgggct ttcatgagat
     9001 accggcaaca actttgataa gcggccgcat agataactga tccagtgtgc tggaattaat
     9061 tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact
     9121 tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg
     9181 gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca
     9241 agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact
     9301 ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta
     9361 gggcggccaa ttccgcccct ctcccccccc ccctctccct cccccccccc ctaacgttac
     9421 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     9481 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     9541 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     9601 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     9661 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     9721 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     9781 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     9841 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     9901 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     9961 ataagcttgc cacaacccgg gaggcgcgcc atgggtgaca gctattgggt ctttgtgaaa
    10021 cgggtgtcca ctagtgaaac cgaaaccgga ggcggctccg gaggcagcgg cggttctgga
    10081 ggttccaccc taccggtctc agctggaggc agtgctggtg gaagtgccgg aggtagaatt
    10141 ctagaaggtg ccgcaagaga ggctgccgct agggaagcag ctgcacgtga ggcagctgct
    10201 agagaagctg cagccaggga ggctgcagct agagcatcag gtggtggcgg ttcaggcgga
    10261 ggtggatctg gaggtggcgg atcgggtggg ggaggttcga caccggtcgc caccatggca
    10321 gaaatcggta ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg
    10381 cactacgtcg atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cg
//
