LOCUS       sequence-426362-        4725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO4C1_STOP
FEATURES             Location/Qualifiers
     source          1..4725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2282..2381)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2398..2417)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2399..2417)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2422..2438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2422..2438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2540..2559)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2551..3360
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2618..2637)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3507..4095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3996..4015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4249..4266
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4425..4452)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4544..4630)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4679..4701
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4693..4710
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4694..4710
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa gagcgccaag
      121 ggcatcgaga acctggcctt cgtgcccagc tcccctgaca tcctgcggag actgtccgcc
      181 tctccaagcc agatcgaggt gtccgccctg tctagcgatc cacagcggga gaattctcag
      241 ccccaggagc tgcagaagcc acaggagccc cagaagtctc cagagccatc tctgccaagc
      301 gccccaccta acgtgagcga ggagaagctg agatccctgt ctctgagcga gttcgaggag
      361 ggcagctatg gctggaggaa ttttcaccct cagtgcctgc agcggtgcaa cacaccaggc
      421 ggcttcctgc tgcactactg cctgctggca gtgacccagg gaatcgtggt gaatggcctg
      481 gtgaacatct ccatctctac agtggagaag cggtatgaga tgaagtcctc tctgaccggc
      541 ctgatcagct cctcttacga catctccttc tgtctgctgt ccctgttcgt gagcttcttc
      601 ggagagaggg gacacaagcc aagatggctg gccttcgccg cctttatgat cggactgggc
      661 gccctggtgt tctctctgcc tcagttcttt agcggcgagt ataagctggg ctctctgttt
      721 gaggatacat gcgtgaccac aaggaatagc acctcctgta caagctccac ctctagcctg
      781 tccaactatc tgtacgtgtt catcctggga cagctgctgc tgggagcagg aggcacccca
      841 ctgtacacac tgggcaccgc ctttctggac gatagcgtgc ccacacacaa gtcctctctg
      901 tatatcggca ccggctacgc catgtccatc ctgggacctg caatcggata tgtgctggga
      961 ggccagctgc tgaccatcta catcgacgtg gccatgggcg agagcacaga tgtgaccgag
     1021 gacgatccta ggtggctggg agcatggtgg atcggcttcc tgctgtcctg gatctttgcc
     1081 tggtctctga tcatcccatt cagctgcttt cctaagcacc tgccaggaac agcagagatc
     1141 caggcaggca agacctccca ggcacaccag tctaacagca atgccgacgt gaagttcggc
     1201 aagtctatca aggattttcc cgccgccctg aagaatctga tgaagaacgc cgtgttcatg
     1261 tgcctggtgc tgagcacaag ctccgaggcc ctgatcacca caggcttcgc cacctttctg
     1321 cccaagttca tcgagaatca gtttggcctg acatctagct ttgccgccac cctgggagga
     1381 gccgtgctga tccctggagc cgccctgggc cagatcctgg gcggcttcct ggtgtccaag
     1441 tttaggatga catgcaagaa taccatgaag ttcgccctgt ttacatccgg cgtggccctg
     1501 accctgtctt tcgtgtttat gtatgccaag tgtgagaacg agccattcgc cggcgtgtcc
     1561 gagtcttaca atggaaccgg agagctggga aacctgatcg caccctgcaa cgccaattgc
     1621 aactgtagcc gctcctacta ttaccccgtg tgcggcgacg gcgtgcagta cttcagccct
     1681 tgctttgcag gatgttccaa tccagtggca cacaggaagc ctaaggtgta ttacaactgc
     1741 tcttgtatcg agagaaagac agagatcacc agcacagccg agaccttcgg ctttgaggcc
     1801 aaggccggca agtgcgagac acactgtgcc aagctgccta tcttcctgtg catcttcttt
     1861 atcgtgatca tcttcacctt tatggccggc acaccaatca ccgtgagcat cctgagatgc
     1921 gtgaaccaca ggcagcggag cctggccctg ggcatccagt ttatggtgct gaggctgctg
     1981 ggaaccatcc ctggaccaat catcttcggc tttacaatcg acagcacctg catcctgtgg
     2041 gacatcaatg attgcggcat caagggcgcc tgttggatct atgataacat caagatggcc
     2101 cacatgctgg tggccatctc cgtgacatgt aaagtgatca ccatgttctt taatggcttc
     2161 gccatctttc tgtacaagcc accccctagc gccacagacg tgtccttcca caaggagaat
     2221 gccgtggtga ccaacgtgct ggccgagcag gatctgaaca agatcgtgaa ggagggctaa
     2281 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2341 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2401 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2461 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2521 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2581 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2641 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2701 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2761 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2821 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2881 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2941 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3001 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3061 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3121 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     3181 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3241 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3301 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3361 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3421 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3481 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3541 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3601 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3661 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3721 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3781 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3841 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3901 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3961 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4021 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4081 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4141 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4201 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4261 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4321 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4381 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4441 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4501 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4561 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4621 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4681 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
