LOCUS       sequence-426364-        3939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC38A7_STOP
FEATURES             Location/Qualifiers
     source          1..3939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1496..1595)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1612..1631)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1613..1631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1636..1652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1636..1652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1754..1773)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1765..2574
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1832..1851)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2721..3309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3210..3229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3463..3480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3639..3666)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3758..3844)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3893..3915
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3907..3924
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3908..3924
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc ccaggtgtct
      121 atcaacaatg actactccga gtgggacctg tctaccgatg caggagagag ggcaagactg
      181 ctgcagagcc catgcgtgga cacagccccc aagagcgagt gggaggcatc cccaggagga
      241 ctggataggg gaaccacaag caccctggga gcaatcttta tcgtggtgaa cgcatgtctg
      301 ggagcaggac tgctgaattt tcctgccgcc ttcagcacag caggaggagt ggcagcagga
      361 atcgccctgc agatgggcat gctggtgttc atcatctccg gcctggtcat cctggcctac
      421 tgctctcagg ccagcaacga gagaacctat caggaggtgg tgtgggccgt gtgcggcaag
      481 ctgacaggcg tgctgtgcga ggtggcaatc gccgtgtata cctttggcac atgtatcgcc
      541 ttccttatca taattgggga tcaacaagac aagatcatcg ccgtgatggc aaaggagcct
      601 gagggagcat ccggaccatg gtacaccgac cggaagttta ccatctccct gacagccttc
      661 ctgtttatcc tgcccctgtc tatccctaga gagatcggct ttcagaagta tgcctccttc
      721 ctgtctgtgg tgggcacctg gtacgtgaca gccatcgtga tcatcaagta tatctggccc
      781 gataaggaga tgacccctgg caacatcctg acaaggccag caagctggat ggccgtgttt
      841 aatgccatgc ctacaatctg cttcggcttt cagtgtcacg tgagctccgt gcccgtgttc
      901 aactccatgc agcagccaga ggtgaagacc tggggaggag tggtgacagc agcaatggtc
      961 atcgccctgg ccgtgtacat gggcaccggc atctgcggct ttctgacatt cggcgcagca
     1021 gtggacccag atgtgctgct gagctatcca tccgaggaca tggcagtggc agtggcaagg
     1081 gcctttatca tcctgtctgt gctgaccagc taccctatcc tgcacttctg tggaagggca
     1141 gtggtggagg gactgtggct gaggtatcag ggagtgccag tggaggagga cgtgggaagg
     1201 gagcggagaa ggcgcgtgct gcagaccctg gtgtggtttc tgctgacact gctgctggcc
     1261 ctgttcatcc ccgatatcgg caaagtgatc tccgtgatcg gaggactggc agcatgcttc
     1321 atcttcgtgt tcccaggcct gtgcctgatc caggccaagc tgtctgagat ggaggaggtg
     1381 aagcccgcct cttggtgggt gctggtgagc tacggcgtgc tgctggtgac cctgggagcc
     1441 tttatcttcg gccagaccac agccaacgcc atcttcgtgg atctgctggc ctaagaccca
     1501 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1561 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1621 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1681 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1741 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1801 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1861 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1921 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1981 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2041 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2101 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2161 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2221 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2281 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2341 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2401 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2461 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2521 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2581 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2641 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3121 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3361 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3481 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3541 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3601 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3661 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3721 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3781 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     3841 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     3901 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
