LOCUS       sequence-426365-        4683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO3A1_STOP
FEATURES             Location/Qualifiers
     source          1..4683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2240..2339)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2356..2375)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2357..2375)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2380..2396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2380..2396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2498..2517)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2509..3318
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2576..2595)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3465..4053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3954..3973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4207..4224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4383..4410)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4502..4588)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4637..4659
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4651..4668
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4652..4668
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgca gggcaagaag
      121 ccaggaggaa gctccggagg aggccggtcc ggcgagctgc agggcgatga ggcccagaga
      181 aacaagaaga agaagaagaa ggtgagctgc ttctctaaca tcaagatctt tctggtgtct
      241 gagtgtgcac tgatgctggc acagggaacc gtgggagcat atctggtgtc tgtgctgacc
      301 acactggagc ggagattcaa tctgcagagc gccgacgtgg gcgtgatcgc atctagcttt
      361 gagatcggca acctggccct gatcctgttc gtgtcttact ttggagcaag gggacacagg
      421 ccaagactga tcggatgcgg aggaatcgtg atggcactgg gcgccctgct gagcgccctg
      481 cccgagttcc tgacccacca gtacaagtat gaggcaggag agatcagatg gggagcagag
      541 ggcagagacg tgtgcgccgc caatggaagc ggaggcgacg agggaccaga cccagatctg
      601 atctgtagga atcgcacagc caccaacatg atgtatctgc tgctgatcgg agcacaggtg
      661 ctgctgggaa tcggagccac ccctgtgcag ccactgggcg tgtcctatat cgacgatcac
      721 gtgaggcgca aggattcctc tctgtacatc ggcatcctgt tcaccatgct ggtgtttgga
      781 cctgcatgcg gcttcatcct gggatccttt tgtacaaaga tctacgtgga cgccgtgttc
      841 atcgatacat ctaacctgga catcacccca gacgatccta ggtggatcgg agcatggtgg
      901 ggaggctttc tgctgtgcgg cgccctgctg ttctttagct ccctgctgat gttcggcttt
      961 ccacagagcc tgccacctca ctccgagcca gcaatggaga gcgagcaggc catgctgtcc
     1021 gagcgggagt atgagagacc taagccaagc aatggcgtgc tgaggcaccc actggagcca
     1081 gactctagcg cctcctgttt ccagcagctg cgcgtgatcc ctaaggtgac caagcacctg
     1141 ctgagcaacc cagtgtttac atgcatcatc ctggccgcct gtatggagat cgcagtggtg
     1201 gcaggattcg cagcctttct gggcaagtac ctggagcagc agttcaatct gaccacatcc
     1261 tctgccaacc agctgctggg catgaccgcc atcccttgcg cctgtctggg catctttctg
     1321 ggcggcctgc tggtgaagaa gctgagcctg tccgccctgg gagcaatccg gatggccatg
     1381 ctggtgaatc tggtgtctac agcctgctat gtgagcttcc tgtttctggg atgtgataca
     1441 ggaccagtgg caggagtgac cgtgccttat ggcaactcta cagcaccagg aagcgccctg
     1501 gacccttaca gcccatgcaa caataactgc gagtgtcaga cagattcctt cacccccgtg
     1561 tgcggagcag acggaatcac ctacctgtct gcctgctttg ccggctgtaa tagcacaaac
     1621 ctgaccggat gcgcatgtct gaccacagtg ccagcagaga atgcaaccgt ggtgcctggc
     1681 aagtgcccat cccctggatg tcaggaggcc ttcctgacat ttctgtgcgt gatgtgcatc
     1741 tgttccctga tcggagcaat ggcacagacc ccatctgtga tcatcctgat ccggacagtg
     1801 tcccctgagc tgaagtctta cgccctgggc gtgctgttcc tgctgctgag actgctgggc
     1861 ttcatcccac cccctctgat ctttggcgcc ggcatcgatt ccacatgcct gttctggtct
     1921 accttttgcg gagagcaggg agcatgcgtg ctgtatgaca acgtggtgta cagatatctg
     1981 tacgtgagca tcgccatcgc cctgaagtcc ttcgccttta tcctgtacac cacaacctgg
     2041 cagtgtctga ggaagaatta taagcggtac atcaagaacc acgagggcgg cctgtctacc
     2101 agcgagttct ttgccagcac actgaccctg gataatctgg gaagggaccc agtgccagca
     2161 aaccagacac accgcaccaa gttcatctac aatctggagg atcacgagtg gtgcgagaac
     2221 atggagtccg tgctgtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     2281 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     2341 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     2401 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2461 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2521 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2581 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2641 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2701 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2761 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2821 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2881 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2941 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     3001 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     3061 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     3121 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3181 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3241 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3301 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3361 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4261 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4321 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4381 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4441 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4501 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4561 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4621 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4681 ccc
//
