LOCUS       sequence-426424-        4487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal 
            fragment (aa 1-153) from Haliangium ochraceum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Steens JA, Bravo JPK, Salazar CRP, Yildiz C, Amieiro AM, Kostlbacher
            S, Prinsen SHP, Andres AS, Patinios C, Bardis A, Barendregt A, 
            Scheltema RA, Ettema TJG, van der Oost J, Taylor DW, Staals RHJ
  TITLE     Type III-B CRISPR-Cas cascade of proteolytic cleavages.
  JOURNAL   Science. 2024 Feb 2;383(6682):512-519. doi: 10.1126/science.adk0378.
            Epub 2024 Feb 1.
  PUBMED    38301007
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adk0378"; journalName: "Science"; date: "2024-02-2-
            2"; volume: "383"; issue: "6682"; pages: "512-519"
COMMENT     Sequence Label: pSAVED-CHAT_PCaspase-N-term
FEATURES             Location/Qualifiers
     source          1..4487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(33..79)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     94..116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        join(123..128,129..146,147..153)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     regulatory      2052..2061
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2292..2310)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2306..2353
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(2501..3292,3293..3361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3124..3143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3362..3466)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3540..4278)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 cgactcctgc attaggaaat gacaaacaac agataaaacg aaaggcccag tctttcgact
       61 gagcctttcg ttttatttga tgcctttaat taaagcggat aacaatttca cacaggaggc
      121 cgtttacact ttatgcttcc ggctcgtata atgtgtgggg aaaccacaac ggtttccctc
      181 tagaaataat tttgtttaac tataagaagg agatatacat atgactcacc atgctgatca
      241 tgactgccag ccgcacaggg agagcgttca gccggatgaa atccagtgta tcctggttct
      301 ggacctgagc atcgataacg caatcaccgc atgtagcgtt accccgcatc tgccccgtgc
      361 tgcacgtcgt gttgaactac atctgaacga ctttggtgca gaacgtgcac cgtacggggg
      421 tgcaagcgat cgtcgtacct ggcgttgttg gatgcaggca gttgatgcaa tgctggctga
      481 cgcacgtgca cagctgggtg cagaagttga atttactcat tattatctgg caggtcgtgc
      541 agcactcccg gtttttgcat atctgggtct gagactgggt aaacaggcaa acatcactac
      601 cgttaaccgt cgtgatgacg gttgttggga tgttgttccg tgccagcgtc cggcttcgag
      661 cgcagcatcg ggggtttcgc ccagcgcacg tttttttgac gaagttcgtg gtctggacac
      721 cgatgagcgt agcagcgaaa gcggtatggt tgcagtttgg gtaagcaccc agcgtgatgt
      781 tgatcgtggg ctgctgcgtg catttgcaag ggcaagaggt gaccgtgacc tggcaggtat
      841 cgttagcctg cgtgcacgtc cggctgcagg tgatgacacc ggtgatatgc gtctcctgga
      901 aggtgcagac ggccccgatg ctgcacgtga actggttaac tgttttcgta gcatcccgaa
      961 ccagtatccg cgtagcagcg gcctgatggt ttttgttagc ggcccggtta ccctggcagc
     1021 aatggttggg cgtgcaatca acccgagaat ccatggcccg gtttggtggc cgtactttcg
     1081 tggtggggag tacgagccgg cactggaata tccgtggccg ctgatcagcg gccccccgag
     1141 gatcctgatc gcaaccgcaa acgctccgga gggtgaaaac ccgactctgg acgttgaggc
     1201 agagctgaag catctggaag aagcactggc agaaccgcgt aaacgtaaac tgtgtgaggt
     1261 acagcgttgc ccggcagcaa ccgtaagcga tatcaccagc gcactgcgta gctttaaacc
     1321 gcatatactg cattttatcg gtcatggtac tgcactcggt gtttatctga ggagcgcaga
     1381 acatgacggt gcacagtttg ttcgtggtga ggatttccag cagatgatcg caacctcgct
     1441 acgtcagaag gaccgtgaaa tgcatctggt tgttctgaac gcttgctgca cccacgaact
     1501 ggcaaaggca ctgaccgaac aggttagctg caccatcggt accgatatcg aagtttatga
     1561 ttcggcaagc atacattttg ctgcacgttt ttatgatcac ctggttcatg gtaccagcgt
     1621 tcattatgca tttaacgcag cagttgatga atgtcgtgca cacagcacca gcggccagga
     1681 agttttttgc ctgcacccgg cagcagaacg tgcaccggca agcgcaaccc cgccggtacg
     1741 tgcagatgaa ctggttttct ttagcccggc agaacgtcat agctaataac atacgcgtaa
     1801 caaggtaagg acaaattaat gatgaaaagg gctctgctga taggtgcacc gctggcacat
     1861 agcgcaaccc atgcactggc aggtgttgca aacgatatcg accgtgcagg tgaaatcctg
     1921 cgtctgtatg gttttcattg tgatgacgtt ctctttggtg aacgtgcaac tagagatggt
     1981 atcgttgcac gtctggaagc actgatcgct gaaacccagg ctgatgatgc tgttgttatc
     2041 tattttagcg gccatggtgg ccgtgttgtt aacaccggga tcgttaaaga tctaccgggt
     2101 ggtgactatc gtcccggcgc tcaccagttc ctggttccgg aagactacca accgaaagca
     2161 cagaccttta ccggtatcct ggacttcgag ctgaggtatc tagttgcacg tctggcagca
     2221 cgtaccgaaa acgttagcgt tatcctggat tgttgtcata gcggtggtgc aataagatga
     2281 ctcgagagcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     2341 gaggggtttt ttggggtcat cgtggccgga tcttgcggcc cctcggcttg aacgaattgt
     2401 tagacagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2641 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2881 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3181 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3241 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3301 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3361 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3421 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3481 aagtgccacc tgtagctagc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg
     3541 cgctgcggac acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt
     3601 gaggcaaaac agcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag
     3661 agttcacata aacagacgct tttccggtgc atctgtggga gccgtgaggc tcaaccatga
     3721 atctgacagt acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggcgggtc
     3781 gctccctctt gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt
     3841 ctcccttacg ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg
     3901 taggtcgttc gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc
     3961 gccttatccg gtaactgttc acttgagtcc aacccggaaa agcacggtaa aacgccactg
     4021 gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc
     4081 tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct
     4141 gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca
     4201 cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct
     4261 caagaagatc ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct
     4321 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
     4381 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     4441 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgc
//
