LOCUS       sequence-426427-        1467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of EcSTH which 
            is a soluble transhydrogenase from E. coli that can be used to 
            increase NADH/NAD+ ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-EcSTH-FLAG
FEATURES             Location/Qualifiers
     source          1..1467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1435..1458
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaattcatgc cccacagcta cgattacgac gccatcgtga tcggcagcgg acccggtgga
       61 gaaggcgctg ccatgggttt agtcaagcaa ggtgctaggg tggccgtcat tgagaggtac
      121 cagaatgtgg gcggcggctg tacccactgg ggaacaatcc ctagcaaagc tttaaggcac
      181 gccgtgtctc gtatcattga gttcaaccag aaccctttat actccgacca ctctcgttta
      241 ttaagaagca gcttcgccga cattttaaac cacgccgata acgtcatcaa ccaacagacc
      301 agaatgaggc aaggtttcta cgagaggaac cactgcgaga ttttacaagg taacgctaga
      361 ttcgtggacg aacacacttt agccctcgac tgccccgatg gctccgtgga gactttaaca
      421 gccgagaagt tcgtgattgc ttgtggcagc agaccctacc accccaccga cgtggacttc
      481 acccaccctc gtatctatga cagcgacagc attttaagca tgcatcacga gcccagacac
      541 gtgctgatct acggcgccgg cgtgatcggc tgcgagtacg ctagcatctt tcgtggaatg
      601 gacgtgaagg tggatttaat caacactcgt gataggctgc tcgcctttct ggatcaagaa
      661 atgagcgatt ctttaagcta ccacttctgg aactccggag tcgtgattcg tcacaacgag
      721 gagtacgaga agattgaggg ctgcgacgac ggcgtgatca tgcatttaaa gtccggcaag
      781 aagctgaagg ccgactgttt actctatgcc aacggtcgta ccggcaacac cgactcttta
      841 gctctccaga acatcggact ggagaccgac tctcgtggcc agctgaaagt gaacagcatg
      901 taccagacag cccagcccca tgtgtacgcc gtgggagatg tgatcggcta cccttcttta
      961 gcttccgccg cttacgacca aggtcgtatc gctgctcaag ctttagtcaa aggagaggcc
     1021 accgcccatt taatcgagga cattcccacc ggcatctaca ccatccccga gatcagcagc
     1081 gtgggcaaga cagagcagca gctgaccgcc atgaaagtcc cttacgaggt gggaagagcc
     1141 cagttcaaac atttagctcg tgcccagatc gtgggcatga acgtgggcac cctcaagatc
     1201 ctctttcatc gtgagaccaa ggagattctg ggcatccact gtttcggaga gagagctgct
     1261 gagatcatcc acattggcca agctatcatg gaacagaagg gcggcggcaa caccatcgag
     1321 tacttcgtga acaccacctt caactacccc accatggccg aagcctatcg tgtggccgcc
     1381 ctcaatggtt taaacagact gttcggtgga tctggtggat ctggtggatc tatggattac
     1441 aaggatgacg atgacaagta aaccggt
//
