LOCUS       sequence-426457-        9647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red #1.4 post-SynapShot and Lyn-miRFP670.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Son S, Nagahama K, Lee J, Jung K, Kwak C, Kim J, Noh YW, Kim E, Lee 
            S, Kwon HB, Heo WD
  TITLE     Real-time visualization of structural dynamics of synapses in live 
            cells in vivo.
  JOURNAL   Nat Methods. 2024 Jan 8. doi: 10.1038/s41592-023-02122-4.
  PUBMED    38191933
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Jan 8. doi: 10.1038/s41592-023-02122-4."
COMMENT     Sequence Label: #1.4 post-R-SynapShot-Lyn-miRFP670
FEATURES             Location/Qualifiers
     source          1..9647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1834..1843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1840..2784
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             2794..2850
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4829..4847)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5097..5116
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5291..5314
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             5848..5874
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5881..5907
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6196..6215)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6261..6316
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6707..6723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6731..6747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7149..7165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7173..7189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7197..7203,7204..7221,7222..7227))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7242..7263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7322..7517
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7368..7503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7523..7657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7560..7579)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7614..7633
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7725..7742)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7896..8484)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7976..7995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8655..9446,9447..9515))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9278..9297
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9516..9620)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgagc
     1741 tcagccacca tgggatgtat aaaatcaaaa gggaaagaca gcgcgggagc agatagtgct
     1801 ggtagtgctg gtagtgctgg agatccaccg gtcgccacca tggtagcagg tcatgcctct
     1861 ggcagccccg cattcgggac cgcctctcat tcgaattgcg aacatgaaga gatccacctc
     1921 gccggctcga tccagccgca tggcgcgctt ctggtcgtca gcgaacatga tcatcgcgtc
     1981 atccaggcca gcgccaacgc cgcggaattt ctgaatctcg gaagcgtact cggcgttccg
     2041 ctcgccgaga tcgacggcga tctgttgatc aagatcctgc cgcatctcga tcccaccgcc
     2101 gaaggcatgc cggtcgcggt gcgctgccgg atcggcaatc cctctacgga gtactgcggt
     2161 ctgatgcatc ggcctccgga aggcgggctg atcatcgaac tcgaacgtgc cggcccgtcg
     2221 atcgatctgt caggcacgct ggcgccggcg ctggagcgga tccgcacggc gggttcactg
     2281 cgcgcgctgt gcgatgacac cgtgctgctg tttcagcagt gcaccggcta cgaccgggtg
     2341 atggtgtatc gtttcgatga gcaaggccac ggcctggtat tctccgagtg ccatgtgcct
     2401 gggctcgaat cctatttcgg caaccgctat ccgtcgtcga ctgtcccgca gatggcgcgg
     2461 cagctgtacg tgcggcagcg cgtccgcgtg ctggtcgacg tcacctatca gccggtgccg
     2521 ctggagccgc ggctgtcgcc gctgaccggg cgcgatctcg acatgtcggg ctgcttcctg
     2581 cgctcgatgt cgccgtgcca tctgcagttc ctgaaggaca tgggcgtgcg cgccaccctg
     2641 gcggtgtcgc tggtggtcgg cggcaagctg tggggcctgg ttgtctgtca ccattatctg
     2701 ccgcgcttca tccgtttcga gctgcgggcg atctgcaaac ggctcgccga aaggatcgcg
     2761 acgcggatca ccgcgcttga gagcggaagc ggagctacta acttcagcct gctgaagcag
     2821 gctggagacg tggaggagaa ccctggacct gagaccgaca ccctcctgct atgggtgctg
     2881 ctgctctggg tgcccggcag caccggcgac gcaccggtcg gaggccaaaa gttggatgat
     2941 gtagacccat tggttactac taactttggc aaaataaggg gaattaagaa agaactcaat
     3001 aatgaaattt tggggcctgt cattcagttt cttggggttc catatgctgc tccaccaaca
     3061 ggagaacatc gatttcagcc tccagaacca ccatctccct ggtctgatat ccggaatgcc
     3121 actcagtttg ctcctgtatg tccccagaat atcattgatg gcagattgcc tgaagttatg
     3181 cttcctgtgt ggttcactaa taacttggat gtggtttcct catatgtgca agaccagagt
     3241 gaagactgtc tatacttaaa catctatgtc ccaactgaag atgtaaaaag aatatccaag
     3301 gaatgtgcca gaaaacccgg caagaaaata tgtagaaaag gagatattcg ggacagtggg
     3361 ggtcccaaac cagtgatggt gtacatccat ggtggctctt acatggaagg tactggaaat
     3421 ctatatgatg ggagtgtgtt ggcaagctac ggtaatgtga tcgtcatcac agtcaactat
     3481 cggcttgggg tacttggctt cttgagcaca ggggatcagg ctgccaaagg aaactacggg
     3541 ctccttgacc tcatccaggc cctcagatgg accagcgaga acattgggtt ctttggtggt
     3601 gaccccttgc gaatcaccgt gtttggatca ggcgctgggg gttcatgtgt caatctgctg
     3661 actttatccc attattctga aaaaggactt tttcaacgag caatagctca gagtggaaca
     3721 gccctttcca gctgggctgt tagtttccag cctgcaaaat acgctagaat tctagccaca
     3781 aaagttggct gcaatgtgtc agatacagta gagttagtag aatgcctgca gaagaagcct
     3841 tacaaagaac ttgttgatca agatgttcaa ccagcccgat accacatagc ctttggacct
     3901 gtgatcgatg gtgatgtaat accagatgac cctcagatac tgatggaaca aggagagttc
     3961 ctcaattatg atatcatgtt aggagttaac caaggggaag ggttgaagtt tgtggaaaac
     4021 atagtagata gtgatgatgg tgtatcagcc agtgattttg actttgctgt ctctaatttt
     4081 gttgataatt tatatggata tcctgaaggc aaagatgttt tgagagaaac cattaaattc
     4141 atgtatactg actgggctga tcgccataac cctgaaacta gaaggaagac attgttggct
     4201 ttgtttacag accatcagtg ggtagcaccc gctgtggcta cagcagacct tcactcgaac
     4261 tttggctcac ctacatactt ctatgccttt tatcatcatt gccaaactga ccaagttcca
     4321 gcttgggctg atgcagctca tggggatgag gttccctatg tattgggaat ccccatgatt
     4381 ggccctacag agttatttcc ttgcaatttc tccaagaatg atgtgatgtt gagtgctgta
     4441 gtaatgacat actggacgaa ttttgctaaa accggtgacc caaatcaacc agttcctcaa
     4501 gacacaaaat tcattcatac caaacccaac cgctttgaag aagtagcatg gaccagatat
     4561 tcccagaaag accaacttta tctccatatt ggattaaaac cgagagttaa agaacattac
     4621 agagccaata aggtaaatct ctggttggag ctggtacctc atctgcataa tctcaatgac
     4681 atttctcaga cgcgtggttc aggtggttca ggagccacca tcaaagagtt catgcgcttc
     4741 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     4801 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     4861 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccgaggc gtacgtgagg
     4921 caccccgccg acatccccga ttacaagaag ctgcccttcc ccgagggctt caagtgggag
     4981 cgcgtgatga acttcgaaga cggcggtctg gtgaccgtta cccaggactc ctccctgcag
     5041 gacggcacgc tgatctgcaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     5101 gtaatgcaga agaagaccat gggctgggag gcctccaccg agatgctgta ccccgaagac
     5161 ggcgtgctga agggccatag ctatcaggcc ctgaagctga aggacggcgg ccactacctg
     5221 gtggagttcg agaccatcta catggccaag aagcccgtgc aactgcccgg cgattactgt
     5281 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5341 gggcgctccg agggccgcca ccgtctgggc atggacgagc ggtacaagtt agggcatggt
     5401 acaggtagca caggcagcgg tagctccggt ggtacgcgtt atacctcgac aacaactaaa
     5461 gtgccatcaa cagacatcac tctcagacct acaaggaaaa attccactcc agtcacatca
     5521 gcctttccca ctgccaagca ggatgatccc aaacaacaac caagtccctt ctcggtagat
     5581 caaagggact actccacaga gctaagtgtc acaatcgcag tgggagcctc tctgctgttt
     5641 ctgaacatct tggcttttgc agccctatac tacaagaaag acaagaggag acatgatgtc
     5701 caccggaggt gcagccctca gcgcaccacg accaacgacc taacccatgc tccagaagag
     5761 gaaattatgt ctctccaaat gaagcacact gacttggatc acgagtgtga gtccatccat
     5821 ccacatgagg tggttcttcg gaccgcctac ccatacgatg ttccagatta cgctgctagc
     5881 tatccttatg acgtgcctga ctatgcccgg accgcctgcc ccccagatta tactcttgct
     5941 atgaggaggt caccggatga tgttccttta atgacaccta acaccatcac aatgattccc
     6001 aacactatac cagggattca gcccttacat acattcaaca catttactgg aggacagaat
     6061 aatactctac ctcatccgca tccacacccc cactcccaca gctaagcggc cgcaattcac
     6121 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     6181 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     6241 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     6301 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     6361 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     6421 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     6481 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     6541 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     6601 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcaccgtcg
     6661 accagctggt cgacggtgca ccgtcgacca gcttggcgta atcatggtca tagctgtttc
     6721 ctgtgtgaaa ttgttatccg ctcacaattc cacacgcact cggaaggaca tatgggaggg
     6781 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     6841 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     6901 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     6961 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     7021 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     7081 gatccctcga cctgcaccgt cgaccagctg gtcgacggtg caccgtcgac cagcttggcg
     7141 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7201 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7261 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     7321 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7381 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7441 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7501 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     7561 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7621 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     7681 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7741 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7801 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7861 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7921 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7981 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8041 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8101 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8161 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8221 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8281 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8341 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8401 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8461 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8521 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8581 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8641 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8701 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8761 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8821 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8881 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8941 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9001 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9061 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9121 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9181 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9241 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9301 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9361 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9421 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9481 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9541 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9601 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgg
//
