LOCUS       sequence-426461-        9394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses green #1.4 post-SynapShot and mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Son S, Nagahama K, Lee J, Jung K, Kwak C, Kim J, Noh YW, Kim E, Lee 
            S, Kwon HB, Heo WD
  TITLE     Real-time visualization of structural dynamics of synapses in live 
            cells in vivo.
  JOURNAL   Nat Methods. 2024 Jan 8. doi: 10.1038/s41592-023-02122-4.
  PUBMED    38191933
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Jan 8. doi: 10.1038/s41592-023-02122-4."
COMMENT     Sequence Label: #1.4 post-G-SynapShot-mScarlet
FEATURES             Location/Qualifiers
     source          1..9394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1751..1760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2353..2362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4814..4840
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4847..4873
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5078..5134
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5150..5845
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5939..5958)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6004..6059
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6450..6466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6474..6490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6896..6912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6920..6936
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6944..6950,6951..6968,6969..6974))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6989..7010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7069..7264
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7115..7250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7270..7404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7307..7326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7361..7380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7472..7489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7643..8231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7723..7742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8402..9193,9194..9262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9025..9044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9263..9367)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgagc
     1741 tcaagcttcg gccaccatgg cacttcccag atgcatgtgg ccaaattatg tttggagagc
     1801 catgatggca tgtgtggtcc acaggggatc gggtgccccg ttgactctct gcttgttggg
     1861 atgtttgcta cagactttcc acgtactctc tcaaaagttg gatgatgtag acccattggt
     1921 tactactaac tttggcaaaa taaggggaat taagaaagaa ctcaataatg aaattttggg
     1981 gcctgtcatt cagtttcttg gggttccata tgctgctcca ccaacaggag aacatcgatt
     2041 tcagcctcca gaaccaccat ctccctggtc tgatatccgg aatgccactc agtttgctcc
     2101 tgtatgtccc cagaatatca ttgatggcag attgcctgaa gttatgcttc ctgtgtggtt
     2161 cactaataac ttggatgtgg tttcctcata tgtgcaagac cagagtgaag actgtctata
     2221 cttaaacatc tatgtcccaa ctgaagatgt aaaaagaata tccaaggaat gtgccagaaa
     2281 acccggcaag aaaatatgta gaaaaggaga tattcgggac agtgggggtc ccaaaccagt
     2341 gatggtgtat atccatggtg gctcttacat ggaaggtact ggaaatctat atgatgggag
     2401 tgtgttggca agctacggta atgtgatcgt catcacagtc aactatcggc ttggggtact
     2461 tggcttcttg agcacagggg atcaggctgc caaaggaaac tacgggctcc ttgacctcat
     2521 ccaggccctc agatggacca gcgagaacat tgggttcttt ggtggtgacc ccttgcgaat
     2581 caccgtgttt ggatcaggcg ctgggggttc atgtgtcaat ctgctgactt tatcccatta
     2641 ttctgaaaaa ggactttttc aacgagcaat agctcagagt ggaacagccc tttccagctg
     2701 ggctgttagt ttccagcctg caaaatacgc tagaattcta gccacaaaag ttggctgcaa
     2761 tgtgtcagat acagtagagt tagtagaatg cctgcagaag aagccttaca aagaacttgt
     2821 tgatcaagat gttcaaccag cccgatacca catagccttt ggacctgtga tcgatggtga
     2881 tgtaatacca gatgaccctc agatactgat ggaacaagga gagttcctca attatgatat
     2941 catgttagga gttaaccaag gggaagggtt gaagtttgtg gaaaacatag tagatagtga
     3001 tgatggtgta tcagccagtg attttgactt tgctgtctct aattttgttg ataatttata
     3061 tggatatcct gaaggcaaag atgttttgag agaaaccatt aaattcatgt atactgactg
     3121 ggctgatcgc cataaccctg aaactagaag gaagacattg ttggctttgt ttacagacca
     3181 tcagtgggta gcacccgctg tggctacagc agaccttcac tcgaactttg gctcacctac
     3241 atacttctat gccttttatc atcattgcca aactgaccaa gttccagctt gggctgatgc
     3301 agctcatggg gatgaggttc cctatgtatt gggaatcccc atgattggcc ctacagagtt
     3361 atttccttgc aatttctcca agaatgatgt gatgttgagt gctgtagtaa tgacatactg
     3421 gacgaatttt gctaaaaccg gtgacccaaa tcaaccagtt cctcaagaca caaaattcat
     3481 tcataccaaa cccaaccgct ttgaagaagt agcatggacc agatattccc agaaagacca
     3541 actttatctc catattggat taaaaccgag agttaaagaa cattacagag ccaataaggt
     3601 aaatctctgg ttggagctgg tacctcatct gcataatctc aatgacattt ctcagacgcg
     3661 tggttcaggt ggttcaggag ccaccatcaa agagttcatg cgcttcaagg tgcgcatgga
     3721 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3781 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     3841 catcctgtcc ccccagttca tgtacggctc cgaggcgtac gtgaggcacc ccgccgacat
     3901 ccccgattac aagaagctgc ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3961 cgaagacggc ggtctggtga ccgttaccca ggactcctcc ctgcaggacg gcacgctgat
     4021 ctgcaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     4081 gaccatgggc tgggaggcct ccaccgagat gctgtacccc gaagacggcg tgctgaaggg
     4141 ccatagctat caggccctga agctgaagga cggcggccac tacctggtgg agttcgagac
     4201 catctacatg gccaagaagc ccgtgcaact gcccggcgat tactgtgtgg acaccaagct
     4261 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgggc gctccgaggg
     4321 ccgccaccgt ctgggcatgg acgagcggta caagttaggg catggtacag gtagcacagg
     4381 cagcggtagc tccggtggta cgcgttatac ctcgacaaca actaaagtgc catcaacaga
     4441 catcactctc agacctacaa ggaaaaattc cactccagtc acatcagcct ttcccactgc
     4501 caagcaggat gatcccaaac aacaaccaag tcccttctcg gtagatcaaa gggactactc
     4561 cacagagcta agtgtcacaa tcgcagtggg agcctctctg ctgtttctga acatcttggc
     4621 ttttgcagcc ctatactaca agaaagacaa gaggagacat gatgtccacc ggaggtgcag
     4681 ccctcagcgc accacgacca acgacctaac ccatgctcca gaagaggaaa ttatgtctct
     4741 ccaaatgaag cacactgact tggatcacga gtgtgagtcc atccatccac atgaggtggt
     4801 tcttcggacc gcctacccat acgatgttcc agattacgct gctagctatc cttatgacgt
     4861 gcctgactat gcccggaccg cctgcccccc agattatact cttgctatga ggaggtcacc
     4921 ggatgatgtt cctttaatga cacctaacac catcacaatg attcccaaca ctataccagg
     4981 gattcagccc ttacatacat tcaacacatt tactggagga cagaataata ctctccctca
     5041 tccgcatcca cacccccact cccacagcgg aagcggagct actaacttca gcctgctgaa
     5101 gcaggctgga gacgtggagg agaaccctgg acctgccccg gggtcgacaa tggtgagcaa
     5161 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa
     5221 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     5281 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc
     5341 tcagttcatg tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta
     5401 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     5461 cgccgtgacc gtgacccagg acacctccct ggaggacggc acgctgatct acaaggtgaa
     5521 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg
     5581 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
     5641 ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc
     5701 caagaagccc gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc
     5761 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac
     5821 cggcggcatg gacgagctgt acaagtgagc ggccgcaatt cactcctcag gtgcaggctg
     5881 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     5941 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     6001 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     6061 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     6121 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     6181 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     6241 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     6301 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     6361 ctgtccctct tctcttatgg agatccctcg acctgcaccg tcgaccagct ggtcgacggt
     6421 gcaccgtcga ccagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6481 ccgctcacaa ttccacacaa catacgccgg aaggacatat gggagggcaa atcatttaaa
     6541 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     6601 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     6661 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     6721 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     6781 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     6841 gcaccgtcga ccagctggtc gacggtgcac cgtcgaccag cttggcgtaa tcatggtcat
     6901 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6961 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7021 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta
     7081 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7141 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7201 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7261 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7381 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     7441 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8701 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9001 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9241 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9301 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9361 ttccgcgcac atttccccga aaagtgccac ctgg
//
