LOCUS       sequence-426465-        9230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dTAG-BromoTag-mAID-EGFP reporter (PiggyBac).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Hatoyama Y, Islam M, Bond AG, Hayashi KI, Ciulli A, Kanemaki MT
  TITLE     Combination of AID2 and BromoTag expands the utility of degron-based
            protein knockdowns.
  JOURNAL   EMBO Rep. 2024 Sep;25(9):4062-4077. doi: 10.1038/s44319-024-00224-4.
            Epub 2024 Aug 23.
  PUBMED    39179892
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44319-024-00224-4"; journalName: "EMBO Rep"; date: 
            "2024-09"; volume: "25"; issue: "9"; pages: "4062-4077"
COMMENT     Sequence Label: pMK459 (dTAG-BromoTag-mAID-EGFP)
FEATURES             Location/Qualifiers
     source          1..9230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1153..2335
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1384..2326
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2283..2303
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2388..2397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2394..2396,2397..2717)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3183..3386
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(3393..3395,3396..3398,3399..4109)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3393..3395,3396..3398,3399..4109)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3438..3459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3699..3718)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4046..4067
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4146..4166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4302..4423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4339..4358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4393..4412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4454..4487
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4548..5047
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4583..4606)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4980..4999
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5108..6127
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5108..6127
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    6690..6723
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   6929..6991
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7042..7062)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7042..7060)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7081..7097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7081..7097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7094..7116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7105..7121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7129..7135,7136..7153,7154..7159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7174..7195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7312..7329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7483..8071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7563..7582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8242..9033,9034..9102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8865..8884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9103..9207)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagtaat gagtaattca tacaaaagga
     1021 ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa cgtagaaccc
     1081 agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg aggtggagaa
     1141 gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     1201 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     1261 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     1321 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     1381 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     1441 gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg
     1501 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     1561 ttgagttgag gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     1621 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     1681 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     1741 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     1801 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     1861 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     1921 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     1981 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     2041 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     2101 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     2161 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     2221 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     2281 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacgcta
     2341 gcccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggcc accatgggag
     2401 tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct
     2461 gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc tcccgggaca
     2521 gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag
     2581 gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct
     2641 atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg
     2701 agcttctaaa actggaaggc ggcggcgctg gtgcaggcgc cggatcctct gtgaaggacg
     2761 tgcccgactc tcagcagcac ccagcaccag agaagagcag caaggtctcg gagcagctca
     2821 agtgctgcag cggcatcctc aaggagatgt ttgccaagaa gcacgccgcc tacgcctggc
     2881 ccttctacaa gcctgtggac gtggaggcac tgggcgccca cgactactgt gacatcatca
     2941 agcaccccat ggacatgagc acaatcaagt ctaaactgga ggcccgtgag taccgtgatg
     3001 ctcaggagtt tggtgctgac gtccgattga tgttctccaa ctgctataag tacaaccctc
     3061 ctgaccatga ggtggtggcc atggcccgca agctccagga tgtgttcgaa atgcgctttg
     3121 ccaagatgcc ggacgagggc ggaggcggag gtggcggcgc tggtgcaggc gcccccacca
     3181 tgaaggagaa gagtgcttgt cctaaagatc cagccaaacc tccggccaag gcacaagttg
     3241 tgggatggcc accggtgaga tcataccgga agaacgtgat ggtttcctgc caaaaatcaa
     3301 gcggtggccc ggaggcggcg gcgttcgtga aggtatcaat ggacggagca ccgtacttga
     3361 ggaaaatcga tttgaggatg tataaagcta gcatggtgag caagggcgag gagctgttca
     3421 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3481 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3541 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3601 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3661 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3721 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3781 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3841 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3901 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3961 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4021 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4081 tcactctcgg catggacgag ctgtacaagg gtgcaggcgc ccaagcttcg aattctgcag
     4141 tcgaccccaa gaagaaaaga aaggtcaggt agggatccac cggatctaga taactgatca
     4201 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4261 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc gatcatattc
     4441 aataaccctt aatataactt cgtataatgt atgctatacg aagttattag gtctgaagag
     4501 gagtttacgt ccagccaagc ttaggatctc gacctcgaaa ttctaccggg taggggaggc
     4561 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     4621 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccgactcc
     4681 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     4741 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     4801 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     4861 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     4921 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     4981 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5041 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccgcta gcatggatag
     5101 atccggaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     5161 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     5221 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     5281 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     5341 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     5401 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     5461 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     5521 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     5581 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     5641 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     5701 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     5761 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     5821 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     5881 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     5941 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     6001 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     6061 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     6121 ggaataggct agccgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     6181 ccatgcatcg atgatatcag atccccggga tgcagaaatt gatgatctat taaacaataa
     6241 agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga
     6301 gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga
     6361 taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt
     6421 ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc tcccactcat
     6481 gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt cccactttgt
     6541 ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc
     6601 agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct aattccatca
     6661 gaagctggtc gagatccgga acccttaata taacttcgta taatgtatgc tatacgaagt
     6721 tattaggtcc ctcgaagagg ttcactagct agttaaaagt tttgttactt tatagaagaa
     6781 attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat
     6841 ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat
     6901 aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa
     6961 cgtacgtcac aatatgatta tctttctagg gttaatctag tatacgcgta tgcggccgct
     7021 taattaatcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     7081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7921 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
