LOCUS       sequence-426466-       15452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual-enSERT-2.2 vector for site-specific integration of.a two-color 
            enhancer-reporter construct into the H11 locus (separated by a 
            synthetic insulator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15452)
  AUTHORS   Hollingsworth EW, Liu TA, Alcantara JA, Chen CX, Jacinto SH, Kvon EZ
  TITLE     Rapid and quantitative functional interrogation of human enhancer 
            variant activity in live mice.
  JOURNAL   Nat Commun. 2025 Jan 6;16(1):409. doi: 10.1038/s41467-024-55500-7.
  PUBMED    39762235
REFERENCE   2  (bases 1 to 15452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-6"; volume: "16(1):409. doi"; pages: " 
            10.1038/s41467-024-55500-7"
COMMENT     Sequence Label: PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11
FEATURES             Location/Qualifiers
     source          1..15452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1302..1318
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1315..1449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1604..2317,2318..2320,2321..2323))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1607..2317,2318..2320,2321..2323))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1649..1670)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1998..2017
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2257..2278
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2320..2329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7059..7769
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7204..7222)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7472..7491
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7918..8052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8049..8065)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10476..10495)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10477..10495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10502..10519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10502..10518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10511..10533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             10656..10958
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10837..10856
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             11307..12101
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11307..12101
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11361..11380)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11971..11990
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(12351..13142,13143..13211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12974..12993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13335..13923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13824..13843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14077..14094
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14211..14232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14247..14252,14253..14270,14271..14277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14285..14301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14290..14312
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14309..14325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14309..14325
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        14346..14364
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaaaaaaaaa aaaaagagta aaggcatagt ctaccacatc cactgaggtg tgttcttaca
       61 gccaaagact ttttctcctg aggtctaagg gaacctcgta gtatgagtct catgacatgg
      121 aatgaatttc ctgttttgtt ttcttggcct tagttaagct aagcagaccc actattctgc
      181 accactcatt agttaggatg ctaacactca tttaagaggt taatgattga aaaggcattt
      241 tcacagaaac catatggcgc tccagtggct ttcattaggt catttgagaa tccagatttt
      301 ctcatcttct ctattcacta caggttgttg gcagttttgg ccagttagct caccttgaaa
      361 atggaaacat gtctgacaag agccttgagc tgaatatcat ccagagtatt tgctagagac
      421 agggtcttaa gtctcattaa attgcttaga agtgtgttta gtcctataaa ctatgtctca
      481 tttgtgtgtc cttcacaaag agcgctagtg tcgatcatca attagcctag cctataaagg
      541 tgacacaacc tggtcagtcc ctctgtatgt ctactatttc cccttctgat ttctactatc
      601 ttctcaagaa ctggttcttc agcttccttt gggaaatgtc actttttaat gatgtgtgtt
      661 ttgcttatgt atatgtctac atactaccca tgtgcttggt cactgcagag gccagaagac
      721 ggtgtcaggt gcactggaac tagagttaat gacagatgtg agccataggg tgctttgttc
      781 acatctttcc agtcccaaat gcgtctaaac ttatgtgata catactagat taccacaaaa
      841 ttgctccaaa gacacctttc tccctctgag atgaatctct ggctggcctt gctctaggca
      901 atcctgtgtt caattcaagg actgaatttg attacatcgg taatccagtg ccccaggcat
      961 ttctgctttt tctgtaaggt tcttatcccc tggaagactg tttacgtatt catttttttt
     1021 tgagactaag tttcaggtag aaaaagcttg ccttgaactt cactatatag ggcttatctt
     1081 gaactcttgt gggtcttcca cctttcttca gttagcttct gtacactgcc agacatgaaa
     1141 atcagatcca tttatagata gatggtcatg agccaccatg tgggtgtcta aaattgatct
     1201 caggacctct gaaagaccag ctagttctct taactgctga gccatctctc tagcgtgtct
     1261 atacacattt aatatcccct tgttcccttt ctgcttgccg ctctagaact agtggatcca
     1321 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     1381 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     1441 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     1501 gaggtttttt aaagcaagta aaacctctac agatgtgata tggtgagtgc tgattatgat
     1561 cattacttat ctagagctta gatcccccct gccctagtta ttactacttg tacagctcgt
     1621 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     1681 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     1741 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     1801 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     1861 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     1921 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     1981 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     2041 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     2101 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     2161 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     2221 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     2281 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcc gccgcgctct
     2341 gcttctggaa ggctgcgctc cgcggcgtgg atgctccggg gaaagttgcg ctccgcggca
     2401 gggatgctcc tgggaaggtt gcgctccgcg gcagggatgc tctggggaag gctggtcctg
     2461 gccgaggatc gggaacgcgc cgctcgctct gcttctcttg tcttcgcttg tctctggatg
     2521 gaaccagatt tggttctgag tagctgtcag cgtctggtga cctgctcgcc gccctgcgcc
     2581 tttaaggagt cttcaccggc cccgcccact ctccgctggg ccaatcagcg agccggagga
     2641 ggccttgggg ccaggaatct tccagcagtt tcgcgtctgg tggagcttcc ccgcctccct
     2701 tgagtaatcg gagttgtggg ttccgccctt gtccagaact ctccagaggt ttctggggtt
     2761 cactggagag tacggattcc tgagggggag ggtgtgggga agtgctggtg ctactagtga
     2821 cactgttgct atggcgacgc attactaagg cctgtgtgga atggacaaga aagatcacct
     2881 ctagctcggt gttgtgtaca gtttgttgtg atttgtgggg tttcgccaac tcgcacagtt
     2941 ctgaatatgg gggttaaagg ctaaaactta agggctaaaa cttctccccg ccaagtttag
     3001 gagacccagg gagatgcctg ggggcgtgtc cggtgacgtg atcctctcca atcgcgttac
     3061 aatggcagtg ctgcctctga cctcatggac taatttagga actagaggct ctgtcccagc
     3121 acaggctcaa agttgccggg aggggcgggg tggggggtgg gggggacccc ggctgctcag
     3181 tttggatgtt cctgaccggt accctgcaca tagcaggggg ttggagctgg gtgagcactg
     3241 tgctcctttg caacccaggc cattctatga ttctgtcatt ctaaatctct ctttcagcct
     3301 aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagggcagc gagaagcgtt
     3361 cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg
     3421 ctcggggatg cggggggagc gtcgggccgg agcggagccc cgggcggctc gctgctgccc
     3481 cctagcggga gagggacgta attacatccc tgggggcttt gggggggggg ctctccccgt
     3541 gagctcccgc ggacgccccc ttccaggacc ttccaggaaa tcttcgctat tacgccagct
     3601 ggcgaaaccc tgcacatagc agggggttgg agctgggtga gcactgtgct cctttgcaac
     3661 ccaggccatt ctatgattct gtcattctaa atctctcttt cagcctaaag ctttttcccc
     3721 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     3781 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     3841 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggagagg
     3901 aacgcaatta cagccctggg ggctttgggg gggggctctc cccatgagct cccgcggacg
     3961 cccccttcca ggaccttcca ggaaagatca taggcctgca tccatcccag aagctaagac
     4021 ctgatggtaa ctgctgaagc aatatttcgt ttcttcataa catctctatt atttcgtttc
     4081 ttcataacat ctctatttta acatgttttc aaatgttagc tcaacttcca acatatttaa
     4141 ataatttatt gtacgttctc ttaacactgt gttataggac tcaattatcc ggtaggaaaa
     4201 gcacaaataa ttggtagtgt cctagtttac tttttttttt tgtcgtatta tcttgggtca
     4261 taggagtatc ccctctgtga aaatcaatta acaatattca acatgctgat attaaaagaa
     4321 aagctttaag gttccaccga gatttgaact cggatcgctg gattcagagt ccagagtgct
     4381 aaccattaca ccatggaacc tccctcgtgg gggctttcaa gattgtgtac attactggac
     4441 attctatttt gcgtgtcttc cacacccgtc acttaaaacg aaaacaaaac aaagtctgct
     4501 gttggatatg gtagacaccg ttatcgagca cttgaaatat atgaattcag atgtattgcc
     4561 agcttctaaa tacacatctt cttttgaagg aaggctttgt acaaacaaaa agacttaaaa
     4621 tttcattaat atttttagaa tgttgattat atgttaaaat aagattttgg tatattaggg
     4681 taagtaaaat agatccgtga aattaatttc gcttgctttt tattctttgt aatgtggcta
     4741 ccagaaaatg taaattcgat aatacggctc gcgtttgtac tgttttaaag tatgtttatt
     4801 accctccacc gtcgtttgct gtattttgtg gaaatcaagt ttctgtatca cctagcttta
     4861 aaaactagta actatttttg ttaccatttt atgcgaagtg cggtacccaa aagcaaagac
     4921 atgccgcccg aacagggact tgaaccctgg accctcagat taaaagtctg atgctctacc
     4981 gactgagcta tccgggctct tcacgagagc tttacttttt gcttataaga gggttctcta
     5041 taggaaaagc caggcttgta gaaccgacag aggattttat ctgtgcagca tagaatattt
     5101 tggcacagat ttggaagcag cgggtgaagc tcgcctgctg ctgattgagc tttttctgcc
     5161 tcccgttctt agagcccccg ccgaggctgc gacgcaggga ctgtaccata gtagaggctg
     5221 gaacagtgcg gcgccggaac cggccgcgcg gggccgctgc gggctatggg cttctctgag
     5281 aggttcctcc ccagtcccta gtggcccaga tcccggacac ctgggctccc gcccaggatc
     5341 ctgcaggccc agggcggtcc tggagcggaa agaatgccac gcggggcatt cagaccctgt
     5401 ttgccggcgc tgtatttcgc tttcctgacc tgccctactc cagagcagag aatgaggtcg
     5461 ggcgtgccaa aggctgggga caaagagggc catgcgtggg gggatcggtg tatggggtga
     5521 cagggagctg ttcctgacca tttgacatgt gctggaatgc gtgtgcgact cacctgcgtg
     5581 tggctgcagt cacgtgtgat ttatgaccaa gtgcaactct ccactgttta gtgattcaca
     5641 cggatgattc acacgtagga atgcaggtgc cctgtgctca tctctgcctg ggaatgggga
     5701 ggaacagatc ttgggggaca ttggggagtg ggcggacaag cactccaggg catcagtccg
     5761 ggccgctgac caggcggagg gcagtttaga acaatggctg gcccatagta aatgccgtgt
     5821 tagtgtgtta gttgctgttc ttccacgtca gaagaggcac agacaaatta ccaccaggtg
     5881 gcgctcagag tctgcggagg catcacaaca gccctgaatt tgaatcctgc tctgccactg
     5941 cctagttgag accttttact acctgactag ctagggggat gtgctgcaag gcgattaatt
     6001 agaacaatgg ctggcccata gtaaatgccg tgttagtgtg ttagttgctg ttcttccacg
     6061 tcagaagagg cacagacaaa ttaccaccag gtggcgctca gagtctgcgg aggcatcaca
     6121 acagccctga atttgaatcc tgctctgcca ctgcctagtt gagacctttt actacctgac
     6181 tagctgcggc cgccaggaac atccaaactg agcagccggg gtccccccca ccccccaccc
     6241 cgcccctccc ggcaactttg agcctgtgct gggacagagc ctctagttcc taaattagtc
     6301 catgaggtca gaggcagcac tgccattgta acgcgattgg agaggatcac gtcaccggac
     6361 acgcccccag gcatctccct gggtctccta aacttggcgg ggagaagttt tagcccttaa
     6421 gttttagcct ttaaccccca tattcagaac tgtgcgagtt ggcgaaaccc cacaaatcac
     6481 aacaaactgt acacaacacc gagctagagg tgatctttct tgtccattcc acacaggcct
     6541 tagtaatgcg tcgccatagc aacagtgtca ctagtagcac cagcacttcc ccacaccctc
     6601 cccctcagga atccgtactc tccagtgaac cccagaaacc tctggagagt tctggacaag
     6661 ggcggaaccc acaactccga ttactcaagg gaggcgggga agctccacca gacgcgaaac
     6721 tgctggaaga ttcctggccc caaggcctcc tccggctcgc tgattggccc agcggagagt
     6781 gggcggggcc ggtgaagact ccttaaaggc gcagggcggc gagcaggtca ccagacgctg
     6841 acagctactc agaaccaaat ctggttccat ccagagacaa gcgaagacaa gagaagcaga
     6901 gcgagcggcg cgttcccgat cctcggccag gaccagcctt ccccagagca tccctgccgc
     6961 ggagcgcaac cttcccagga gcatccctgc cgcggagcgc aactttcccc ggagcatcca
     7021 cgccgcggag cgcagccttc cagaagcaga gcgcggcgat ggtgagcaag ggcgaggagg
     7081 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     7141 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     7201 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     7261 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     7321 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     7381 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     7441 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     7501 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     7561 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     7621 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     7681 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     7741 ccggcggcat ggacgagctg tacaagtaat aataactagg gcagggggga tctaagctct
     7801 agataagtaa tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa
     7861 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     7921 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7981 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8041 catgtctgga tccactagtt ctagagcggc agtaagtagt tctctattgg cagtttgaca
     8101 catcctgccc ttaccttact accactgttg gctcagcaga cacccaggat aagtgcacta
     8161 gtgttccttt cctgaccagt gggactgctt tttccagatt cctcttgaag ctgaggaggc
     8221 agtgagagca tgatggcatc taatgagctt ggaagtacca gactgccctg atccacagcc
     8281 aggttttgct gaaaagtgac cagtttgtcc tcctccagta gagtgggcag ctgaaggatt
     8341 ataatctact gtcaagactt ggaggcccct gcagtcaaag tccaatagaa tattatgaaa
     8401 tggagaatgg cttattttaa tctctatagt ggaattaaaa tagcatttat ggccccagat
     8461 ccataattaa tccaatgact ggtcaaatta gcttgaacct gactgaaaaa cctgcaatca
     8521 gtatagtatc tttcagaatg ctttacattc aatttataat accagacttc aagttgtaag
     8581 ttaacaattt tgagagaaac taatgcagca gaggcaaggg aaagacttaa attaatgtga
     8641 ccattaatta gttggagtca ggctggatta acattgtgcc agtaaatttc agaaaaatta
     8701 ctatatgact tctctgtaaa ctttgatttt gtagagtata atattgattc cttgatactt
     8761 gccacaagct actttcaggg ttagtccagc ttaaactaat gctatcagaa ctttatgtgc
     8821 agaagaaatt ccagaagtcc taaggtaaaa ttaaaacgtg aaaggaactc atttagactg
     8881 tcttagttca tggaagaaat aaacacagta ccagccatat cgttcacaca cgtggcatat
     8941 gtaactttta ataaaccaaa agaaaaagtc ccaaatattc aagtgaaaaa aatccaaaca
     9001 gttgacacaa gcctaactga tagttacttt atgtacagct gtatgtataa gttcaaaaaa
     9061 agctacccta tttgtatgta caaaagttta tacacaggtc tgtacataag ggtctataca
     9121 ttttattttt tcagaaccct taggtgtcac ctctagaaga caccaacact tcattcacat
     9181 attttataaa agaaagactt ccaggactga caatcttgta tcccttgtat ttgaaccatg
     9241 taggttcatt cgatttaaca gccttctgtc ataatgtaga tcatggtagg tatgtggatc
     9301 ccctaaacat cttcacatag tgtgctccaa tgtaaaagcc cattacaaag agggaatgag
     9361 agtcatgacc agggtgtcac cacaggtcca ggtatgggtg agccagcctg ccctcttcac
     9421 tgtctatatt ccagttcatc tacactgatg actttggtgg gcatggaggg cagaggctgc
     9481 tgtagcacat ctgagccaaa ccatttggca aggcccacag gggagctgct cctttggctg
     9541 gtacgatgct ccagctggga gtgcatgtgg ggcatgcccg accggctggg tacattctga
     9601 ggagtctgaa cgctgatagc tgctgcctgg gaactagagc ctggaggatg cagaactatg
     9661 gagagaagag tgtcagtgca gcaaagtagc tctactctac acctcaccca cagaacccca
     9721 caagtctact aagaccaccc ttggatactc actcccaagt cctgttctca gtattgacaa
     9781 tattttctga cctactttgc ttgaatggtt tgttactttg ggggatggga agtctaccag
     9841 aggatttaac caaaggcttc ctaaagtgca aaatgcaacg taagaaaatg caacgtaaga
     9901 aagcccacca aggctgacat gttcacatct cagcaaaaat tctcaggctt ataagaagtg
     9961 aaaaacagag ctagagcatg gctggctccg actgtccttg cagtagtaat aatccctgag
    10021 actgcacagg tcagttcact tcacctgttt ttctagtgac acctaagaaa caaaggggca
    10081 gtgataacta ttgctcctgg aaatctcaga actttccagt gtgatcagct aacctggcct
    10141 atttctaaac cttacctgag cgctgtagct gctgctgcat caccgccaaa tgcagagggg
    10201 tcccaggacg agggtttagg agagggtgac tagcagcaga gaccaaaggg tacaagggtt
    10261 gacccaggag tgggccagat agcccctgta aaggagccat gtctatccca ggaggaagca
    10321 cacctgatgg ggagaaagaa gaaactcatg agttgtagat gttcctaaaa atgtcaaagt
    10381 ttactacttt tgttgatttg gatacttaca aggaggtgga tgtggtgaac acttgccacc
    10441 atacatgtac caggggcgcg cctaaattca attcgcccta tagtgagtcg tattacaatt
    10501 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    10561 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    10621 gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac acctataaaa
    10681 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgccggggc
    10741 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
    10801 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
    10861 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
    10921 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg agattatcaa
    10981 aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa cggtgctgac
    11041 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
    11101 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
    11161 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
    11221 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agctctgatc
    11281 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
    11341 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
    11401 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
    11461 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
    11521 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    11581 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    11641 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    11701 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    11761 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    11821 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    11881 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    11941 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    12001 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    12061 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattattaac gcttacaatt
    12121 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc
    12181 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    12241 atgtatccgc tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    13021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13261 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    13321 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    13381 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    13441 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    13501 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    13561 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    13621 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    13681 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    13741 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    13801 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    13861 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    13921 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    13981 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    14041 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    14101 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    14161 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    14221 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    14281 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    14341 ctcagaatta accctcacta aagggactag tcctgcaggt ttaaacgaat tcgcgcgcat
    14401 tgactgcagg ctgagtgagt gggggtacgt aaggacccaa agctatgtag cctagcttct
    14461 caaggtgtct aaagaaagga agggtagatt accaatgagc agagtttggg cttctctatt
    14521 tttaaagttt taaaatgttt atatcaaggc aaggtttctg ttgtccttgc tggtctggaa
    14581 ctagattggc cttgacctaa gagatgatgc gacttctgcc tcccaagtgc taattaaagg
    14641 tgtgagtcac cacgcttgcc ttggtgtttt ctatttatag gttatatatt catttttcct
    14701 attgcatgtc acttcccaaa ttacatctca tctagtcatc tgtgcaccca atcacaaata
    14761 ggcataacct ggtagatttc cactaaaagt tacttagagg aagtttgtga atttattatt
    14821 acaggcaagc catggaaatg tgtgtagctg ttgcagttgg tttgaggagg tgtaagtgca
    14881 tggtgtattt ggtagagaga ctaggctgcc tagttcatta tgagaagata tgtacaacac
    14941 aaacgaagta gggtctcagg ttcaactgtt aagctttctt gcctcaggct gctggagagt
    15001 tgcactcgta cttattcact caattcattc tttggccagg tgggtggtgg tggtggtggt
    15061 tcacaccttt agtcatagtg ttgaggaggc agaggcaaga ggttctgtgt ttgaggccag
    15121 ctgggtctat agattgaatt ctaggaaagc cagggctaca tagagaaacc tgttttcaaa
    15181 agaaaaaaga aaaaaagaaa agacgaatgg tgtatgtgtg tgtttgtcag tcatctgtct
    15241 ggaattgaat ttgagatccc cctgcctccc aagtgtcctg attaaaggca tagtctatat
    15301 agctgggagt ggtggcgcac acttttaatt ctagcacttg agaggcagag gcaggcggat
    15361 ctctgagttc gaggccagcc tggtcaacaa agtgagttcc aggacagcca aggctacaca
    15421 gagaaaccct gtctcgaaaa aacccaaaaa aa
//
