LOCUS       sequence-426477-        8316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA targeting CD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, 
            Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA
  TITLE     Engineered CRISPR-Cas12a for higher-order combinatorial chromatin 
            perturbations.
  JOURNAL   Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0.
  PUBMED    38760567
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0."
COMMENT     Sequence Label: gCH27 (crCD55-4)
FEATURES             Location/Qualifiers
     source          1..8316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             414..594
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    672..793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(709..728)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     763..782
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      833..968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(989..1007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1017..1034)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1017..1033)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1026..1048)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1175..1630
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1262..1281)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1472..1493
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1656..1760
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1761..1829,1830..2621)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1979..1998)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2792..3380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3281..3300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3534..3551
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3668..3689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3704..3709,3710..3727,3728..3734)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3742..3758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3747..3769
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3766..3782
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3766..3782
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3801..3821
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3803..3821
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3849..4075
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4076..4256
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4303..4428
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4921..5154
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5339..5383
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5564..5681
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5765..5976
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5996..6005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6005..6028
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6029..6031,6032..6034,6035..6745)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6029..6031,6032..6034,6035..6745)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6074..6095)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6335..6354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6682..6703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6755..6811
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6812..7606
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6866..6885)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7476..7495
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7622..8210
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7675..7695)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8093..8104)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagt ggacccagag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
      301 atttctactc ttgtagatac tggtattgcg gagccacgag gaatttctac tcttgtagat
      361 tttttttaag cttggcgtaa ctagatcttg agacactgct ttttgcttgt actgggtctc
      421 tcttgttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      481 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      541 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
      601 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
      661 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      721 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
      781 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
      841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      901 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
      961 tggaggccta gggacgtacc caattcgtcc tatagtgagt cgtattacgc gcgctcactg
     1021 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1081 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     1141 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     1201 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     1261 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     1321 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     1381 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     1441 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     1501 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     1561 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     1621 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1741 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1801 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1861 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1921 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1981 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2041 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2101 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2161 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2221 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2281 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2341 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2401 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2461 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2521 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2581 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2641 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2701 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3421 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3481 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3541 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3601 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3661 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3721 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3781 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     3841 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     3901 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     3961 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     4021 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     4081 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4141 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4201 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4261 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     4321 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     4381 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     4441 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     4501 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     4561 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     4621 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     4681 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     4741 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     4801 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     4861 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     4921 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     4981 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     5041 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     5101 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     5161 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     5221 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     5281 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     5341 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     5401 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     5461 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     5521 atcgtttcag aatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     5581 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     5641 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     5701 gcagagatcc actttggcgc cggctcgagg gggcccgggt cgagtggctc cggtgcccgt
     5761 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5821 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5881 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5941 gttctttttc gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgccac
     6001 catggattac aaagacgatg acgataagat ggtgagcaag ggcgaggagc tgttcaccgg
     6061 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     6121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     6181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     6241 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     6301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     6361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     6421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     6481 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     6541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     6601 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6721 tctcggcatg gacgagctgt acaagggcag cggcgccacc aacttcagcc tgctgaagca
     6781 ggccggcgac gtggaggaga accccggccc catgcttgaa caagatggat tgcacgcagg
     6841 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6901 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6961 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     7021 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     7081 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     7141 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     7201 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     7261 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     7321 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     7381 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     7441 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     7501 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7561 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaacgc gttaagtcga
     7621 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7681 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7741 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7801 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7861 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7921 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7981 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     8041 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8101 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8161 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     8221 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8281 ctggaagggc taattcactc ccaacgaaga caagat
//
