LOCUS       sequence-426478-        8442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA array targeting CD81, B2M, KIT, CD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, 
            Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA
  TITLE     Engineered CRISPR-Cas12a for higher-order combinatorial chromatin 
            perturbations.
  JOURNAL   Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0.
  PUBMED    38760567
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0."
COMMENT     Sequence Label: gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)
FEATURES             Location/Qualifiers
     source          1..8442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             540..720
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    798..919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(835..854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     889..908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      959..1094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1115..1133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1143..1160)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1143..1159)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1152..1174)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1301..1756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1388..1407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1598..1619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1782..1886
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1887..1955,1956..2747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2105..2124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2918..3506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3407..3426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3660..3677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3794..3815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3830..3835,3836..3853,3854..3860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3868..3884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3873..3895
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3892..3908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3892..3908
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3927..3947
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3929..3947
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3975..4201
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4202..4382
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4429..4554
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5047..5280
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5465..5509
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5690..5807
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5891..6102
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6122..6131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6131..6154
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6155..6157,6158..6160,6161..6871)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6155..6157,6158..6160,6161..6871)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6200..6221)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6461..6480)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6808..6829
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6881..6937
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6938..7732
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6992..7011)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7602..7621
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7748..8336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7801..7821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8219..8230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagt ggacccagag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
      301 atttctactc ttgtagatac tggtattgcg gagccacgag gaatttctac tgtcgtagat
      361 atataagtgg aggcgtcgcg ctgaattcct actctcgtag gttctgcgtt ctgctcctac
      421 tgcttaattt ctactctagt agatggcgcg acccccagga aggtctcaat ttctcctcta
      481 ggagattttt tttaagcttg gcgtaactag atcttgagac actgcttttt gcttgtactg
      541 ggtctctctt gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      601 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      661 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      721 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
      781 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      901 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
      961 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     1021 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1081 cttttttgga ggcctaggga cgtacccaat tcgtcctata gtgagtcgta ttacgcgcgc
     1141 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1201 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1261 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     1321 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1381 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1441 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     1501 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     1561 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     1621 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     1681 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     1741 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1801 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1861 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1921 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1981 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2041 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2101 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2161 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2221 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2281 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2341 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2401 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2461 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2521 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2581 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2641 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2701 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2761 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2821 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2881 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2941 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3001 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3061 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3121 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3181 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3241 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3301 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3361 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3421 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3481 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3541 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3601 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3661 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3721 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3781 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3841 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     3901 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     3961 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     4021 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     4081 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     4141 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     4201 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4261 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4321 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4381 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     4441 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     4501 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     4561 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     4621 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     4681 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     4741 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     4801 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     4861 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     4921 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     4981 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     5041 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     5101 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     5161 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     5221 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     5281 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     5341 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     5401 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     5461 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     5521 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     5581 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     5641 accattatcg tttcagaatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     5701 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     5761 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     5821 gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtcgag tggctccggt
     5881 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5941 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6001 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6061 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc
     6121 cgccaccatg gattacaaag acgatgacga taagatggtg agcaagggcg aggagctgtt
     6181 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     6241 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     6301 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     6361 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     6421 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     6481 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     6541 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     6601 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     6661 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     6721 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     6781 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     6841 gatcactctc ggcatggacg agctgtacaa gggcagcggc gccaccaact tcagcctgct
     6901 gaagcaggcc ggcgacgtgg aggagaaccc cggccccatg cttgaacaag atggattgca
     6961 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7021 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7081 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     7141 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     7201 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     7261 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     7321 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     7381 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     7441 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     7501 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     7561 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     7621 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     7681 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaacgcgtta
     7741 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7801 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7861 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7921 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7981 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     8041 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     8101 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     8161 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     8221 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     8281 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     8341 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8401 gggggactgg aagggctaat tcactcccaa cgaagacaag at
//
