LOCUS       sequence-426484-       12424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. 
            Plasmid can also be used to make lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 12424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU- dio PSD95-mCherry- W
FEATURES             Location/Qualifiers
     source          1..12424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1265..1298)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1349..1382)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1403..2113)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1681..1700)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1950..1968
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(4274..4303)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      4336..4345
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4353..4386
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4437..4470
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4495..5083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4548..4568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4966..4977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5086..5102)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5087..5103)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5608..5788
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5814..5831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5820..6044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6090..6518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6177..6196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6387..6408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6527..6547)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6532..6861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6712..6847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6774..6793
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6909..6956
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6975..7349
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7479..7600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7516..7535)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7570..7589
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7649..7665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7649..7665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7662..7684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7673..7689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7697..7703,7704..7721,7722..7727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7742..7763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7880..7897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8131..8150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8810..9601,9602..9670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9433..9452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9671..9775)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9850..9869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10041..10420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10422..10620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10575..10595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10638..10818
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10865..10990
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11483..11716
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11901..11945
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12243..12360
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatcctcta gagtcgactc
     1261 cggaataact tcgtatagga tactttatac gaagttatgc agaatggtag ctggattgta
     1321 gctgctatta gcaatatgaa acctcttaat aacttcgtat agcatacatt atacgaagtt
     1381 atggcgcgcc ttgacgaccg gtttacttgt acagctcgtc catgccgccg gtggagtggc
     1441 ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
     1501 ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg
     1561 tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct
     1621 cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg
     1681 tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt
     1741 agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
     1801 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg
     1861 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga
     1921 tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc
     1981 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
     2041 cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc
     2101 ccttgctcac catgaattcg agtctctctc gggctgggac ccagatgtag gggcctgaga
     2161 ggtcttcaat gacacgtttc actttgtgat agatctcttc aaagctgtcg ccctctacga
     2221 tggctgagaa gcactctgtg aactcctgct ccagcttcgt ggctctgtcg aaggctttcc
     2281 gggcttgctc ctctgtgatc cgcttattga tctctagcac attctccagg gagcggggac
     2341 ggatgaagat ggcgatgggg tgcaggtggg ccgcctgcag ccgccgcacg gcattggccg
     2401 agacatcgag gatgcagtgc ttcccctgct ctgctacctc tcgcacagac tggacgctgg
     2461 tcccatagag gtggctgttg tactggccag cctcaatgaa cttgtgtgcc tggatgtcct
     2521 tctccatttt ctcccgggag gagacaaagt ggtaatcctg gccgtctatc tcatattccc
     2581 gcttaggacg tgtcgtatga gggacacagg atccaaactt gtcggggaac tcggagagaa
     2641 gatcatcgtt ggcacggtct ttggtgggtc caaggatgat gatgggacga gcatagtgca
     2701 cttccatctg ggtcaccgtc tcatagctca gaaccgagtc ttctcgaccc tgtgatccag
     2761 agctggagcc ccagtccttg gcctttaacc ttgaccactc tcgtcgctcg acccgccgtt
     2821 tgctgggaat gaagccaatg tcgtcggtct cactgtcgga gtggacccgc cgtgcttgcc
     2881 accactcttc gtcaccaacg tcaatgacat gaagcacatc cccgaagcgg aagctcaggg
     2941 cctggctcaa gaaaccgcag tccttggtct tgtcgtaatc aaacagggcc ctaatgtaga
     3001 agcccctctt ggggttgctt cgcaaggatg cagtccctga gcctaggcta ctattcatga
     3061 gctgttcccg aagatcatgg atcttggcct cgaatcgact atactcttct ggtttatact
     3121 gagcgatgat cgtgaccgtc tgacccgcat tcttcagggc aatggcagcc tgttcgtgac
     3181 tggcattgcg gaggtcaaca ccattgaccg acaggatctg gtcccccttc cgtagctccc
     3241 cactgaggtc ggctggaccc ccagcaagga tgaaggagat gaagatgcct tcaccatcct
     3301 cgccgcccac gatgttgaag cccaggccgg tggagccccg atggatcacg atccgccttg
     3361 gttcccgggg aatgtcttcc tcccccagca ggtccttggc cacaggggag tagcgccgag
     3421 gggaagtggg ggtcatggct gtggggtagt cagtgcccaa gtagctgcta tgactgatct
     3481 cattgtccag gtgctgagaa tacgaggttg tgatgtctgg gggagcatag ctgtcactca
     3541 ggtaggcatt gctgggcttg gccaccttta ggtacacaac gtcatatgtg ttcttcaggg
     3601 ctgccacggc atcctcgtgc ataacgtcct ccagccccac actgttgacc gccaggatct
     3661 tgtctccaat ctgcaacctg ccatccttgt gagcagcacc tccttcgatg atcttcgtta
     3721 catagatgct gttatctcca gggatgtgct ggttcccaac gccccccgca atgctgaagc
     3781 caagtccttt aggccctttg atgagtttga tctccatgac cttttcggct gggggtttcc
     3841 ggcgcatgac atagtggcga acgatggaac ctgcctcttt gagggcctcc accgcagctg
     3901 aatgggtcac ctcccgaaca tccacttcat ttacaaacag gatgctgtca ttgaccctga
     3961 ggcggccatc ctgggctgca gccccaccag gaatgatctt ggtgataaaa atggacgggt
     4021 cgtcaccgat gtgcgggttg tcagtgccac ctgcgatgct gaagcccaga cctgagttac
     4081 ccctttccaa tgtgatctcc tcatactcca tctccccctc tgttccattc acctgcaact
     4141 catatcctgg ggcttctagg gtgtccgtgt tgacaatcac agggggagaa ttggcctggt
     4201 tggggaggtg ggccgggctg tgttccagag ggggcgtgtc ttcatcttgg tagcggtatt
     4261 tcttggcgcg ccccaagtcc tcttcagata tcagcttttg ctcggcgcgc cgggttgtca
     4321 ctatacagag acagtccatg gtggcggcta gcataacttc gtataaagta tcctatacga
     4381 agttatttgc cttaacccag aaattatcac tgttattctt tagaatggtg caaagaataa
     4441 cttcgtataa tgtatgctat acgaagttat gaattcgata tcaagcttat cgataatcaa
     4501 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4561 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4621 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4681 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4741 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4801 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4861 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4921 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4981 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5041 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     5101 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     5161 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     5221 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5281 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     5341 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     5401 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     5461 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     5521 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     5581 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5641 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5701 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5761 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     5821 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5881 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5941 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6001 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6061 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6121 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6181 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6241 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6301 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6361 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6421 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6481 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6541 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6601 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6661 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6721 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6781 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6841 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6901 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     6961 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     7021 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     7081 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     7141 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     7201 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     7261 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     7321 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     7381 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7441 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7621 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7741 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7861 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8461 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8941 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9721 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9781 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     9841 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     9901 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     9961 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
    10021 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
    10081 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    10141 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    10201 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    10261 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
    10321 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
    10381 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    10441 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
    10501 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
    10561 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
    10621 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    10681 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    10741 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    10801 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
    10861 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
    10921 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
    10981 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    11041 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    11101 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    11161 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    11221 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    11281 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
    11341 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    11401 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    11461 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    11521 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    11581 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    11641 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    11701 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    11761 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    11821 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    11881 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    11941 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    12001 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    12061 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    12121 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    12181 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
    12241 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
    12301 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    12361 cgggtttatt acagggacag cagagatcca gtttggttaa ttaacccgtg tcggctccag
    12421 atct
//
