LOCUS       sequence-426487-       13255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 Nsp3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 13255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: CMV5.1-SARS-CoV-2 Nsp3
FEATURES             Location/Qualifiers
     source          1..13255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3266..3290)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3297..3362)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     LTR             3375..3643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    3647..3667
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3714..3738)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3735..3938)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3764..3784)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3939..4242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(4253..4273)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     4423..4442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6177..6281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6282..6350,6351..7142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6500..6519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7313..7901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7802..7821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8055..8072
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8189..8210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8225..8230,8231..8248,8249..8255)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8263..8279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8268..8290
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8287..8303
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8287..8303
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8352..8473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8363..8382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8417..8436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8647..9441)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8758..8777)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9368..9387
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9508..9837)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9522..9657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9576..9595)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9822..9842
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(9851..10279)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9961..9982)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10173..10192
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    10325..10549
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10538..10555
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        10575..10593
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10575..10592
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    10655..10679
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taagagttgt ccttcttgta gtagttgtcc agcttagggt cgatttcggt gcacaccacg
       61 ccgtccagct tgtaggtcac gggcttgatg gtggtggtgt agctgttctc cttgtagaac
      121 acgtcggtga tgggaccctt gtattcagag gacttggtca gcagggctcc gtcgatgcag
      181 tacagggtct ccttagaggt gatgtgcttg tagtgtccgc actggtagtt gccggtgtac
      241 tcggaagcgc aggtgaaggt gccgtgcttc agttcgtact gggctggagg agcggacatc
      301 atcacgaatg ggctttcctg ctgcaccagg tacttggtgg cctgctttcc gcaggtgcaa
      361 gggatctgca cgcccttctt gaactgctcg taggacaggg tacccatgta catcacagct
      421 tccactccct tcagggtggt ctgctgctgg ccgcaggtct tgcacaccac gttcagcaca
      481 cgcttgcagg agtccaggtt ggcgtgctgg aacaggtagc tcatggtctc tctcacgtct
      541 cccagttcgc ccacggtctt gttgcagtag gccaggatca gagcgcagaa gttagcggcc
      601 tcgccagcac gggctctgta gtaagcgtcc tgcagagcgg gtgggttgaa cttcagttcg
      661 atctgctgca gggtcagcag agcggtggcc aggtagcagt tgttgtcggc ccacttgatg
      721 ctggtcagtc cgttcacctg ggggtacttc cacttcttgg tgtggttcag ggcagacatg
      781 tacctgccca ggaaggatgg gtcggtggtg tggtagtatt cgaaagcctc cactctcagg
      841 gtgtcatcgt taggcagcac gtagaaggtc ttgccctcgt gggagttgtg tggcttgatc
      901 ttggtcacgt cagctccgtc caggtaggta ggaccgaact gctgtccgta ggtcatgctc
      961 atgtccacca cctgggtgtg caggttgatg ttgtccacgg tggtgaacac cttgatggtc
     1021 ctcacctcgc gcagagacag cagggtcttc aggttgtcga aggtgatcac ttcaccgtcc
     1081 aggtggaagg tggttgggtt ggaggtgtag tacacgctct tgtctccgcg cttcaggaac
     1141 tcgatgccca gctgggtgga ctgtccgctg taagaccagt ccttgtagga accggccagg
     1201 ctgatggttt cgatgaagtg ttcctcaggg gtcttgctag aggaggtcag gtatccgttg
     1261 taggcggtca cagcgtcagg gctgctcacg ctcacggtag caggcacctt cagggaacgc
     1321 atgtatctag cggcttcctc caggttcaga ccgtgggtca cgtatcccag tggcatggtc
     1381 accagggtct cgttcaggtc gttcagggtg ttgatcagag aggccacggt ggtcttggag
     1441 gtgtagaagt agaaacgagc gccgtagtcc accacaccct cctggatctt gatgcccttg
     1501 tactttctct ggatggtgct cacgatggcc ttggtctcca cgcacacggg catcagcttc
     1561 ctggtttcct cagcgtgggc cagcatttcg cgcaggttcc agctcacggt gcccaggatt
     1621 tcctgcttct cgttagagat gatggagggc aggatgtaga aggcagactt gcacttcttc
     1681 agcacggtct tagcttcctc cacggtgtaa ccgttcaggc cctgaccggg gtaggtggtg
     1741 atgtagttgt cggttggcac cttacgcaga gccttggcca gcatctcggt ggtgccaccg
     1801 gccttcttgg tagggatgac cacagcggtc agcactcctt cctgcaccac gtcgcccacg
     1861 atgtaggggg cgtccttctt caggaaggtg atgtcgatgt cagacaccag ggtagcggag
     1921 tcagggtgca ggttgccgtt gatgtcgatg tacagcagca ggttctcggt caggaacttg
     1981 gtttcctcca gggtggtggt cacttcctcc acgcaggcct tgatcttctt gtcgtcctgc
     2041 tttctctgct ccacgctggg cttagattcg gtgatgaaag gcttcacttc ctccttgggg
     2101 atctcggcga tcttctgctc cacctgcttt tcggacttca tctccaggaa ggagctcacc
     2161 agcttgtcgt acaggttctt gtcgaacacg gccaggtaca cgttggtcct cacggtgtcc
     2221 acgcacacgc gcaggctgtg gatgggatca gcaccgaaga ttccggcaga cagcagtgga
     2281 gccagcagca cctcgtgctg gttgaagttt tcgtaggcgc tcttcagcag ctggatgtcc
     2341 tctcccttgt tcacgtttgg tcccaccacg tgcaggcagt gcttggccag gttgtggcca
     2401 gacagcacgc aggatccgcc caccttcaga ggaccgttgg tagcgatgta gtcgtcggat
     2461 tccacctgca tggcgttgtt ggtagccttg ttcagagctc cagccacacc tccgccgtgc
     2521 ttcaggtaca cgttagcggc gttcaccacc acggttggtt tcaccttctt ggcttcctcc
     2581 acgatgtcag cgttcttgat gtacacgttg tcggtcagct tcaggtagcc ggagaagctg
     2641 ttcacctcga tggtctgcac cactggggtc agttccatct ccagctgagg ctgcacctcc
     2701 acgatggtct ggatggtggt ggtctggttg tcttcagagc cgtcctgctg acccacggtc
     2761 tgctgggagt cgtcgtccag ccagtcttcc tcctgttcct cttcaggctg cagagcggca
     2821 gaggtggctc cgaattccag aggcttgccc tggtagtcgt cttcggtgcc gtactcgtac
     2881 tgagtggaag gctcgaactc ttcctcttcg cagtcaccct cttcctcgtc ttcgtcggga
     2941 gggtagaagc tgcagtacat gtgagaggcc agcttgaatt cgccgctctc gtcgaacagg
     3001 tagtaggtag ccatagacca ctcgtccagg tcgataccca gaggggtcag cagttcggac
     3061 actggctgca gggtcttgat cacagcgtcg gccaccacgc aggcgaattc gttcacctcg
     3121 gtgcccagct ccacggtgta agcggagcac ttttcgttca gcaccttgtc gatgcgttcg
     3181 tccagctcga aggtgatgtt cacgctcttg taaccctgca cctcgatcac ggtgtcgtct
     3241 ccgaaggtca ccttggtagg ggccatgcca acttttttgt acaaagttgt gctgcccttg
     3301 tcatcgtcat ccttataatc gatgtcatga tccttataat caccgtcgtg gtctttgtag
     3361 tcgcccatgg tgccgatctg taacggcgca gaacagaaaa cgaaacaaag acgtagagtt
     3421 gagcaagcag ggtcaggcaa agcgtggaga gccggctgag tctaggtagg ctccaaggga
     3481 gcgccggaca aaggcccggt ctcgacctga gctttaaact tacctagacg gcggacgcag
     3541 ttcaggaggc accacaggcg ggaggcggca gaacgcgact caaccggcgt ggatggcggc
     3601 ctcaggtagg gcggcgggcg cgtgaaggag agatgcgagc ccctcgccac tttgtataca
     3661 aaagttggct gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga
     3721 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     3781 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
     3841 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     3901 tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     3961 acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag
     4021 gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga
     4081 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     4141 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     4201 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcctgcagg gccaactttt
     4261 ctatacaaag ttgtgatcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc
     4321 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
     4381 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
     4441 tactgagagt gcaccatagg ggatcgggag atccccagca ggaaaaggac aagcagcgaa
     4501 aattcacgcc cccttgggag gtggcggcat atgcaaagga tagcactccc actctactac
     4561 tgggtatcat atgctgactg tatatgcatg aggatagcat atgctacccg gatacagatt
     4621 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     4681 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
     4741 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
     4801 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
     4861 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
     4921 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
     4981 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
     5041 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
     5101 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
     5161 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
     5221 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
     5281 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
     5341 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
     5401 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
     5461 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
     5521 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
     5581 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
     5641 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
     5701 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
     5761 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
     5821 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
     5881 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
     5941 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
     6001 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
     6061 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
     6121 ataaaggcgg atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg
     6181 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6241 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6301 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6361 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6421 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6481 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6541 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6601 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6661 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6721 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6781 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6841 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6901 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6961 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7021 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7081 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7141 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7201 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7381 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7441 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7561 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7621 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7681 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7741 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7801 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7861 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7921 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7981 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8041 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8101 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8161 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8221 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8281 ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta
     8341 tacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     8401 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     8461 caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     8521 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     8581 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     8641 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     8701 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     8761 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     8821 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     8881 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     8941 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     9001 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     9061 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     9121 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     9181 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     9241 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     9301 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     9361 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     9421 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatccgaa
     9481 aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     9541 tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa
     9601 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     9661 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     9721 gcctgctggg gagcctaggg actttccaca cctggttgct gactaattga gatgcatgct
     9781 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     9841 ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     9901 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     9961 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
    10021 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
    10081 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
    10141 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
    10201 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
    10261 gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca
    10321 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
    10381 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
    10441 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
    10501 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
    10561 cagcgagctc tagcatttag gtgacactat agaatagggc cctctagatg catgctcgag
    10621 cggccgccag tgtgatggat atctgcagaa tttcacaact ttgtacaaga aagttgggta
    10681 ttatccgccc ttcagggcga tcttggtggt caccacgttc accacctgtc tggtggtagc
    10741 gcaggtcagc ttgaagggca ggttgttctt cttagcggcg ctcctgatct gcttgcgcag
    10801 ctgctcggac aggctcatga agtccttcac gttccagatc agggcgatgt tgtgagactt
    10861 ggccacctga gcgttgatgt gacgggcgga gcagtcgatg caagctccca ggtctcttgg
    10921 ggtcatgttc tccaccttgt tgtaggtcag catgtagttg ttgcaggagt cgccggtcac
    10981 ttcgatgtcg ctctggtgag acagcttcag gcactccacc acgtctttgg tttccacgtc
    11041 agagtccacg aaaccctgcc tagcggcgga gatgaaggtg ctcagcacgt tgtccaggga
    11101 cacgttctta gccagttcag cctcagcagt agccaccagg gtcttcagct tctccatagg
    11161 cacgttgaag gtagaggaga aggtgttcac gtaggcgtcg aacatcttca cggccacttc
    11221 ggcgctgtct cccacgtcag acaccagagc ctggtccagc agcaggattg gctggcacat
    11281 cagctggctg tagtacacgc tggcggactt ggcgctagat tcctcgcact tggactttcc
    11341 gtcgaacacg atcacgttga taggcaggct gcccttggtg ttgttggccc tcaggttgtc
    11401 caggttcacg aagtggctca gagagtggcg ctcgtaggtc ttctggccag ccttgtcgaa
    11461 gtacaggtgg atgctaccgt tcttcacggt cacagagtcc acgatgtagg agctctggtc
    11521 ggtggggttg attggacgct tgaactgcag gctcaggtct ctggccacct cgtcagagat
    11581 gaaggtggag ccagcgcaga aggtgtcgca gttcacgcag ttccagttgt gcagcttgca
    11641 gaatcccttg ccgccgttgg cgtacacgta gaaggacctg cgcacgccgt tcacgatggt
    11701 ggtgcactcc accctggtag ccctgttgcg cttgtagcac atcatgcagg tagaggagtt
    11761 gcaaccgtcc accacgtgca cgtaggactt ccacacgtag tagaagctgg cgaagaagat
    11821 gtacatgcgc accatggcag agatgggagc catctgcacc aggttgatga tcagccacat
    11881 cagccaggag ttgctgatga agtgcacagc gaagtaggag aagaacagct gcatgatagc
    11941 ggccaggccc agcacgtaga agaacctggt gaacaggatg taggccagga accactcagc
    12001 caccagaccg aaggcggtca ggtcccactt gaagctagag atggtgatct ggatggtttc
    12061 cagggagggg taggtgtcca ggctgtccag tccagacagg cacacggagc atgggatgct
    12121 gccggtgcag taggtggcga tggtcacgtt ggtgctgttc aggtatccct ctctgtaacc
    12181 agtgcagtag ctaggcatac ccaggttaga catcagcact cccagagcgg cggtagagta
    12241 gatcagggaa cccaggcaca cggagagcag caggaaccag atgatgatgt tgatcagctt
    12301 gctgaagttt ggagacttca ggtagttgaa ggaagcctcc aggcagaact ttcccacgct
    12361 cttcacggtg ttcttggcga tggtggtagg catgctagcc ttgatacggg agttggtgct
    12421 tctggtgaag gtgcacagct gcagcagcag ggtgaagaag tagggcatgt agttggtgca
    12481 cacgcggttc agacatctgg tcacgatgtt ggtggtggtg gacaccacct tgttcaggaa
    12541 aggcttggcg tagttagcga tggtgtccca tggcacagag ttcacagcag ccagaccgtg
    12601 agtagccagg gtcttcagac ccagcaccct gctcagctcg ttgggcttct tgatggtcag
    12661 ggagctgttg tccacgtaag cggccatcag gtcggtgtga cccacttcct cggtgatctt
    12721 caggctgttg ttagctggct tcaggatgat gtctcccacc acctcggtgg tcttcacgtt
    12781 gcattccagc acgtccttct ggatggttgg gttctccacc acttcctcgc tcacgggctt
    12841 caggtcttcg caggccaggt tgtccatgcc ctgagcgtcc tcgctcttca gcacgtcgaa
    12901 agagttggag gtttccactg gcttggtaga ccacaggcaa cggatgcacc aggtgttagg
    12961 cttgtaggtg gccttgttgg tagcgttgtt cacgtgccac acgatgggct tgtgcagcag
    13021 cttggctccc ttcttgaagc ttggggtgta gtgcttgtag tcgatagcca ccacgtcgcc
    13081 gttcaggtct gggaagaagg tcaccttcag ctctctagag gcaggcttct tgtatccggt
    13141 cagctggttc aggtcgtcag cgaacttgat gttgtcgcac acgaacttga agttgtcgaa
    13201 gctggcgtta gggtagggct ggtttggcac caggtcgata ggctgctcgg tgaag
//
