LOCUS       sequence-426498-        1665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains luciferase under doxycycline inducible 
            control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-LUC
FEATURES             Location/Qualifiers
     source          1..1665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..1659
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(73..91)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaattcatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctagag
       61 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
      121 acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa
      181 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
      241 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
      301 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
      361 aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac
      421 gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa aacggattac
      481 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
      541 tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaactcc
      601 tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga
      661 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
      721 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
      781 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag
      841 gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc
      901 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct
      961 ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa
     1021 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     1081 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     1141 gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg
     1201 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     1261 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt tgaccgcttg
     1321 aagtctttaa ttaaatacaa aggataccag gtggcccccg ctgaattgga gtcgatattg
     1381 ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt
     1441 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     1501 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     1561 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     1621 ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa ccggt
//
