LOCUS       sequence-426502-        1419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains LbNOX under doxycycline inducible control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-LbNOX-FLAG
FEATURES             Location/Qualifiers
     source          1..1419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1387..1410
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaattcatga aggtcaccgt ggtcggatgc acccatgccg gcaccttcgc catcaagcaa
       61 atcctcgctg agcaccctga cgccgaggtc accgtctacg agaggaacga tgtgatctcc
      121 ttcctgtcct gtggcatcgc cctctacctg ggcggaaaag tggccgatcc ccaaggcctc
      181 ttctacagct cccctgaaga actgcagaag ctgggcgcta atgtgcagat gaaccacaac
      241 gtgctggcca tcgaccctga ccaaaagacc gtcacagtcg aggacctcac caatcacgcc
      301 cagaccaccg agtcctacga caaactggtg atgacctccg gaagctggcc tatcgtgccc
      361 aaaatccccg gcatcgacag cgatagggtg aagctctgca agaattgggc ccacgcccag
      421 gctctgattg aggacgccaa ggaggccaag aggatcaccg tcatcggcgc cggatacatc
      481 ggagccgaac tggccgaggc ctactccaca acaggccacg acgtcaccct gattgacgcc
      541 atggctaggg tcatgcccaa gtacttcgat gccgacttca ccgacgtcat cgaacaggac
      601 tacagggacc atggcgtgca actcgctctg ggcgagacag tggagagctt caccgacagc
      661 gccaccggcc tcacaatcaa gacagacaag aactcctatg agaccgacct ggccatcctc
      721 tgcattggct ttaggcccaa cacagacctg ctgaaaggca aagtggacat ggcccctaac
      781 ggcgccatca ttaccgacga ctacatgagg tccagcaacc ctgatatttt cgctgctggc
      841 gactccgccg ccgtccatta caaccccaca caccaaaacg cctacattcc cctcgctacc
      901 aacgccgtca ggcagggaat cctcgtcgga aagaacctcg tcaagcccac agtgaagtac
      961 atgggaaccc agtccagctc cggactggcc ctctatgaca ggacaattgt ctccacaggc
     1021 ctcacactgg ccgccgccaa gcaacaaggc ctcaatgccg agcaggtcat cgtggaggac
     1081 aactataggc ccgagttcat gccttccacc gagcccgtcc tcatgagcct ggtcttcgac
     1141 cccgatacac acagaatcct gggaggcgcc ctgatgtcca aatacgacgt gtcccagagc
     1201 gctaataccc tgtccgtctg catccagaac gagaacacca tcgatgacct ggccatggtg
     1261 gacatgctgt tccagcccaa tttcgacagg cccttcaact acctgaacat tctcgcccag
     1321 gctgcccaag ctaaagtggc ccaatccgtc aacgctggtg gatctggtgg atctggtgga
     1381 tctatggatt acaaggatga cgatgacaag taaaccggt
//
