LOCUS       sequence-426503-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dTAG-BromoTag-mAID-EGFP reporter (TOL2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Hatoyama Y, Islam M, Bond AG, Hayashi KI, Ciulli A, Kanemaki MT
  TITLE     Combination of AID2 and BromoTag expands the utility of degron-based
            protein knockdowns.
  JOURNAL   EMBO Rep. 2024 Sep;25(9):4062-4077. doi: 10.1038/s44319-024-00224-4.
            Epub 2024 Aug 23.
  PUBMED    39179892
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44319-024-00224-4"; journalName: "EMBO Rep"; date: 
            "2024-09"; volume: "25"; issue: "9"; pages: "4062-4077"
COMMENT     Sequence Label: pMK467 (dTAG-BromoTag-mAID-EGFP)
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..54
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(69..74,75..92,93..99)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     112..134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     131..147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     131..147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     166..186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        168..186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(243..254,255..442)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        464..843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        845..1120
                     /label=chicken beta-actin promoter
     intron          1121..2137
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2061..2083
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2145..2164
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2214..2223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2220..2222,2223..2543)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3009..3212
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(3219..3221,3222..3224,3225..3935)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3219..3221,3222..3224,3225..3935)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3264..3285)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3525..3544)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3872..3893
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3972..3992
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4148..4269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4185..4204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4239..4258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4300..4333
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4394..4893
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4429..4452)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4826..4845
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4954..5973
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4954..5973
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    6536..6569
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(6600..6762,6763..6774)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6804..6823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6805..6823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6833..6850)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6833..6849)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6842..6864)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6991..7445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7077..7096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7287..7308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7471..7575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7576..7644,7645..8436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7794..7813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8607..9195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9096..9115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9349..9366
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
       61 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
      181 taaagggaac aaaagctgga gctcggatct gctgggcttg ctgaaggtag ggggtcaaga
      241 accagaggtg taaagtactt gagtaatttt acttgattac tgtacttaag tattattttt
      301 ggggattttt actttacttg agtacaatta aaaatcaata cttttacttt tacttaatta
      361 cattttttta gaaaaaaaag tactttttac tccttacaat tttatttaca gtcaaaaagt
      421 actttttttt tggagatcac ttgggccctc tagatgcatg ctcgacattg attattgact
      481 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      541 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      601 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      661 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      721 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      781 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      841 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      901 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      961 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
     1021 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     1081 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     1141 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1201 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     1261 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     1321 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
     1381 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     1441 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     1501 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     1561 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     1621 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     1681 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     1741 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     1801 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     1861 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     1921 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     1981 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     2041 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     2101 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     2161 tgtgctgtct catcattttg gcaaagaatt cgagctcggt acctcggtcg acggccacca
     2221 tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc
     2281 agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt gattcctccc
     2341 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg
     2401 aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt
     2461 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg
     2521 atgtggagct tctaaaactg gaaggcggcg gcgctggtgc aggcgccgga tcctctgtga
     2581 aggacgtgcc cgactctcag cagcacccag caccagagaa gagcagcaag gtctcggagc
     2641 agctcaagtg ctgcagcggc atcctcaagg agatgtttgc caagaagcac gccgcctacg
     2701 cctggccctt ctacaagcct gtggacgtgg aggcactggg cgcccacgac tactgtgaca
     2761 tcatcaagca ccccatggac atgagcacaa tcaagtctaa actggaggcc cgtgagtacc
     2821 gtgatgctca ggagtttggt gctgacgtcc gattgatgtt ctccaactgc tataagtaca
     2881 accctcctga ccatgaggtg gtggccatgg cccgcaagct ccaggatgtg ttcgaaatgc
     2941 gctttgccaa gatgccggac gagggcggag gcggaggtgg cggcgctggt gcaggcgccc
     3001 ccaccatgaa ggagaagagt gcttgtccta aagatccagc caaacctccg gccaaggcac
     3061 aagttgtggg atggccaccg gtgagatcat accggaagaa cgtgatggtt tcctgccaaa
     3121 aatcaagcgg tggcccggag gcggcggcgt tcgtgaaggt atcaatggac ggagcaccgt
     3181 acttgaggaa aatcgatttg aggatgtata aagctagcat ggtgagcaag ggcgaggagc
     3241 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3301 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3361 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3421 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3481 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3541 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3601 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3661 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3721 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3781 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     3841 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3901 ccgggatcac tctcggcatg gacgagctgt acaagggtgc aggcgcccaa gcttcgaatt
     3961 ctgcagtcga ccccaagaag aaaagaaagg tcaggtagtc gacggtaccg cgggcccggg
     4021 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     4081 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4141 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4201 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4261 tgtatcttaa cgcgtcgatc atattcaata acccttaata taacttcgta taatgtatgc
     4321 tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagcttag gatctcgacc
     4381 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     4441 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     4501 tccaccggta ggcgccaacc gactccgttc tttggtggcc ccttcgcgcc accttctact
     4561 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     4621 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     4681 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     4741 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     4801 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     4861 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgagcag
     4921 ctgaagctta ccgctagcat ggatagatcc ggaaagcctg aactcaccgc gacgtctgtc
     4981 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     5041 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     5101 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     5161 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     5221 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     5281 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     5341 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     5401 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     5461 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     5521 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     5581 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     5641 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     5701 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     5761 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     5821 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     5881 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     5941 cgccccagca ctcgtccgag ggcaaaggaa taggctagcc gcccgcccca cgacccgcag
     6001 cgcccgaccg aaaggagcgc acgaccccat gcatcgatga tatcagatcc ccgggatgca
     6061 gaaattgatg atctattaaa caataaagat gtccactaaa atggaagttt ttcctgtcat
     6121 actttgttaa gaagggtgag aacagagtac ctacattttg aatggaagga ttggagctac
     6181 gggggtgggg gtggggtggg attagataaa tgcctgctct ttactgaagg ctctttacta
     6241 ttgctttatg ataatgtttc atagttggat atcataattt aaacaagcaa aaccaaatta
     6301 agggccagct cattcctccc actcatgatc tatagatcta tagatctctc gtgggatcat
     6361 tgtttttctc ttgattccca ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt
     6421 ttcatagcct gaagaacgag atcagcagcc tctgttccac atacacttca ttctcagtat
     6481 tgttttgcca agttctaatt ccatcagaag ctggtcgaga tccggaaccc ttaatataac
     6541 ttcgtataat gtatgctata cgaagttatt aggtccctcg aagaggttca ctaagatctt
     6601 tcttgctttt acttttactt ccttaatact caagtacaat tttaatggag tactttttta
     6661 cttttactca agtaagattc tagccagata cttttacttt taattgagta aaattttccc
     6721 taagtacttg tactttcact tgagtaaaat ttttgagtac tttttacacc tctgtcaaga
     6781 accatatgcc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6841 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6901 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6961 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     7021 cgggtgtggt ggttacgcgc agcgtgacca ccacacccgc cgcgccctag cgcccgctcc
     7081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7201 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7261 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7321 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7441 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7501 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7561 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7621 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7681 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7741 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7801 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7861 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7921 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7981 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8041 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8101 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8161 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8221 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8281 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8341 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8401 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9361 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9421 cattaatgca gctggcacga caggtttccc
//
